Antibody stability: A key to performance - Analysis, influences and improvement
<p>An antibody’s stability greatly influences its performance (i.e. its specificity and affinity). Thus, stability is a major issue for researchers and manufacturers, especially with the increasing use of antibodies in therapeutics, diagnostics and rapid analytical platforms. Here we review an...
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2020
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| _version_ | 1864513505268334592 |
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| author | Hui Ma (290168) |
| author2 | Ciarán Ó’Fágáin (19672597) Richard O’Kennedy (713891) |
| author2_role | author author |
| author_facet | Hui Ma (290168) Ciarán Ó’Fágáin (19672597) Richard O’Kennedy (713891) |
| author_role | author |
| dc.creator.none.fl_str_mv | Hui Ma (290168) Ciarán Ó’Fágáin (19672597) Richard O’Kennedy (713891) |
| dc.date.none.fl_str_mv | 2020-09-12T15:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1016/j.biochi.2020.08.019 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Antibody_stability_A_key_to_performance_-_Analysis_influences_and_improvement/27021595 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Immunology Pharmacology and pharmaceutical sciences Antibody stability Protein stability measurement Protein expression Antibody engineering Formulation |
| dc.title.none.fl_str_mv | Antibody stability: A key to performance - Analysis, influences and improvement |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p>An antibody’s stability greatly influences its performance (i.e. its specificity and affinity). Thus, stability is a major issue for researchers and manufacturers, especially with the increasing use of antibodies in therapeutics, diagnostics and rapid analytical platforms. Here we review antibody stability under five headings: (i) measurement techniques; (ii) stability issues in expression and production (expression, proteolysis, aggregation); (iii) effects of antibody format and engineering on stability and (iv) formulation, drying and storage conditions. We consider more than 100 sources, including patents, and conclude with (v) recommendations to promote antibody stability.</p><h2>Other Information</h2> <p> Published in: Biochimie<br> License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.biochi.2020.08.019" target="_blank">https://dx.doi.org/10.1016/j.biochi.2020.08.019</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_3868a13b49a12d9d4ef1aaf1318e712f |
| identifier_str_mv | 10.1016/j.biochi.2020.08.019 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/27021595 |
| publishDate | 2020 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Antibody stability: A key to performance - Analysis, influences and improvementHui Ma (290168)Ciarán Ó’Fágáin (19672597)Richard O’Kennedy (713891)Biomedical and clinical sciencesImmunologyPharmacology and pharmaceutical sciencesAntibody stabilityProtein stability measurementProtein expressionAntibody engineeringFormulation<p>An antibody’s stability greatly influences its performance (i.e. its specificity and affinity). Thus, stability is a major issue for researchers and manufacturers, especially with the increasing use of antibodies in therapeutics, diagnostics and rapid analytical platforms. Here we review antibody stability under five headings: (i) measurement techniques; (ii) stability issues in expression and production (expression, proteolysis, aggregation); (iii) effects of antibody format and engineering on stability and (iv) formulation, drying and storage conditions. We consider more than 100 sources, including patents, and conclude with (v) recommendations to promote antibody stability.</p><h2>Other Information</h2> <p> Published in: Biochimie<br> License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.biochi.2020.08.019" target="_blank">https://dx.doi.org/10.1016/j.biochi.2020.08.019</a></p>2020-09-12T15:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1016/j.biochi.2020.08.019https://figshare.com/articles/journal_contribution/Antibody_stability_A_key_to_performance_-_Analysis_influences_and_improvement/27021595CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/270215952020-09-12T15:00:00Z |
| spellingShingle | Antibody stability: A key to performance - Analysis, influences and improvement Hui Ma (290168) Biomedical and clinical sciences Immunology Pharmacology and pharmaceutical sciences Antibody stability Protein stability measurement Protein expression Antibody engineering Formulation |
| status_str | publishedVersion |
| title | Antibody stability: A key to performance - Analysis, influences and improvement |
| title_full | Antibody stability: A key to performance - Analysis, influences and improvement |
| title_fullStr | Antibody stability: A key to performance - Analysis, influences and improvement |
| title_full_unstemmed | Antibody stability: A key to performance - Analysis, influences and improvement |
| title_short | Antibody stability: A key to performance - Analysis, influences and improvement |
| title_sort | Antibody stability: A key to performance - Analysis, influences and improvement |
| topic | Biomedical and clinical sciences Immunology Pharmacology and pharmaceutical sciences Antibody stability Protein stability measurement Protein expression Antibody engineering Formulation |