Production of Polyhydroxyalkanoates by Two Halophilic Archaeal Isolates from Chott El Jerid Using Inexpensive Carbon Sources
<div><p>The large use of conventional plastics has resulted in serious environmental problems. Polyhydroxyalkanoates represent a potent replacement to synthetic plastics because of their biodegradable nature. This study aimed to screen bacteria and archaea isolated from an extreme enviro...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , |
| منشور في: |
2020
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| الموضوعات: | |
| الوسوم: |
إضافة وسم
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| _version_ | 1864513524046233600 |
|---|---|
| author | Manel Ben Abdallah (18105505) |
| author2 | Fatma Karray (6084419) Sami Sayadi (6084425) |
| author2_role | author author |
| author_facet | Manel Ben Abdallah (18105505) Fatma Karray (6084419) Sami Sayadi (6084425) |
| author_role | author |
| dc.creator.none.fl_str_mv | Manel Ben Abdallah (18105505) Fatma Karray (6084419) Sami Sayadi (6084425) |
| dc.date.none.fl_str_mv | 2020-01-08T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3390/biom10010109 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Production_of_Polyhydroxyalkanoates_by_Two_Halophilic_Archaeal_Isolates_from_Chott_El_Jerid_Using_Inexpensive_Carbon_Sources/25341793 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Biochemistry and cell biology polyhydroxyalkanoate biodegradable polymer hypersaline lake PHA-producing archaea carbon sources |
| dc.title.none.fl_str_mv | Production of Polyhydroxyalkanoates by Two Halophilic Archaeal Isolates from Chott El Jerid Using Inexpensive Carbon Sources |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <div><p>The large use of conventional plastics has resulted in serious environmental problems. Polyhydroxyalkanoates represent a potent replacement to synthetic plastics because of their biodegradable nature. This study aimed to screen bacteria and archaea isolated from an extreme environment, the salt lake Chott El Jerid for the accumulation of these inclusions. Among them, two archaeal strains showed positive results with phenotypic and genotypic methods. Phylogenetic analysis, based on the 16S rRNA gene, indicated that polyhydroxyalkanoate (PHA)-producing archaeal isolates CEJGTEA101 and CEJEA36 were related to Natrinema altunense and Haloterrigena jeotgali, respectively. Gas chromatography and UV-visible spectrophotometric analyses revealed that the PHA were identified as polyhydroxybutyrate and polyhydroxyvalerate, respectively. According to gas chromatography analysis, the strain CEJGTEA101 produced maximum yield of 7 wt % at 37 °C; pH 6.5; 20% NaCl and the strain CEJEA36 produced 3.6 wt % at 37 °C; pH 7; 25% NaCl in a medium supplemented with 2% glucose. Under nutritionally optimal cultivation conditions, polymers were extracted from these strains and were determined by gravimetric analysis yielding PHA production of 35% and 25% of cell dry weight. In conclusion, optimization of PHA production from inexpensive industrial wastes and carbon sources has considerable interest for reducing costs and obtaining high yield.</p><p> </p></div><h2>Other Information</h2> <p> Published in: Biomolecules<br> License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/biom10010109" target="_blank">https://dx.doi.org/10.3390/biom10010109</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_388b4e219bee6aa6fe991f2d79d70d42 |
| identifier_str_mv | 10.3390/biom10010109 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25341793 |
| publishDate | 2020 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Production of Polyhydroxyalkanoates by Two Halophilic Archaeal Isolates from Chott El Jerid Using Inexpensive Carbon SourcesManel Ben Abdallah (18105505)Fatma Karray (6084419)Sami Sayadi (6084425)Biological sciencesBiochemistry and cell biologypolyhydroxyalkanoatebiodegradable polymerhypersaline lakePHA-producing archaeacarbon sources<div><p>The large use of conventional plastics has resulted in serious environmental problems. Polyhydroxyalkanoates represent a potent replacement to synthetic plastics because of their biodegradable nature. This study aimed to screen bacteria and archaea isolated from an extreme environment, the salt lake Chott El Jerid for the accumulation of these inclusions. Among them, two archaeal strains showed positive results with phenotypic and genotypic methods. Phylogenetic analysis, based on the 16S rRNA gene, indicated that polyhydroxyalkanoate (PHA)-producing archaeal isolates CEJGTEA101 and CEJEA36 were related to Natrinema altunense and Haloterrigena jeotgali, respectively. Gas chromatography and UV-visible spectrophotometric analyses revealed that the PHA were identified as polyhydroxybutyrate and polyhydroxyvalerate, respectively. According to gas chromatography analysis, the strain CEJGTEA101 produced maximum yield of 7 wt % at 37 °C; pH 6.5; 20% NaCl and the strain CEJEA36 produced 3.6 wt % at 37 °C; pH 7; 25% NaCl in a medium supplemented with 2% glucose. Under nutritionally optimal cultivation conditions, polymers were extracted from these strains and were determined by gravimetric analysis yielding PHA production of 35% and 25% of cell dry weight. In conclusion, optimization of PHA production from inexpensive industrial wastes and carbon sources has considerable interest for reducing costs and obtaining high yield.</p><p> </p></div><h2>Other Information</h2> <p> Published in: Biomolecules<br> License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/biom10010109" target="_blank">https://dx.doi.org/10.3390/biom10010109</a></p>2020-01-08T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3390/biom10010109https://figshare.com/articles/journal_contribution/Production_of_Polyhydroxyalkanoates_by_Two_Halophilic_Archaeal_Isolates_from_Chott_El_Jerid_Using_Inexpensive_Carbon_Sources/25341793CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/253417932020-01-08T03:00:00Z |
| spellingShingle | Production of Polyhydroxyalkanoates by Two Halophilic Archaeal Isolates from Chott El Jerid Using Inexpensive Carbon Sources Manel Ben Abdallah (18105505) Biological sciences Biochemistry and cell biology polyhydroxyalkanoate biodegradable polymer hypersaline lake PHA-producing archaea carbon sources |
| status_str | publishedVersion |
| title | Production of Polyhydroxyalkanoates by Two Halophilic Archaeal Isolates from Chott El Jerid Using Inexpensive Carbon Sources |
| title_full | Production of Polyhydroxyalkanoates by Two Halophilic Archaeal Isolates from Chott El Jerid Using Inexpensive Carbon Sources |
| title_fullStr | Production of Polyhydroxyalkanoates by Two Halophilic Archaeal Isolates from Chott El Jerid Using Inexpensive Carbon Sources |
| title_full_unstemmed | Production of Polyhydroxyalkanoates by Two Halophilic Archaeal Isolates from Chott El Jerid Using Inexpensive Carbon Sources |
| title_short | Production of Polyhydroxyalkanoates by Two Halophilic Archaeal Isolates from Chott El Jerid Using Inexpensive Carbon Sources |
| title_sort | Production of Polyhydroxyalkanoates by Two Halophilic Archaeal Isolates from Chott El Jerid Using Inexpensive Carbon Sources |
| topic | Biological sciences Biochemistry and cell biology polyhydroxyalkanoate biodegradable polymer hypersaline lake PHA-producing archaea carbon sources |