Leveraging Machine and Deep Learning Algorithms for hERG Blocker Prediction

<p dir="ltr">The human ether-a-go-go-related (hERG) gene is crucial in enabling the regulation of repolarisation process in the heart. Some chemicals act as hERG blockers, resulting in prolonged QT intervals. Predicting the binding capability of molecules with hERG channels is expect...

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Main Author: Syed Mohammad (21075689) (author)
Other Authors: Vaisali Chandrasekar (16904526) (author), Omar Aboumarzouk (18427923) (author), Ajay Vikram Singh (22501868) (author), Sarada Prasad Dakua (22501871) (author)
Published: 2025
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author Syed Mohammad (21075689)
author2 Vaisali Chandrasekar (16904526)
Omar Aboumarzouk (18427923)
Ajay Vikram Singh (22501868)
Sarada Prasad Dakua (22501871)
author2_role author
author
author
author
author_facet Syed Mohammad (21075689)
Vaisali Chandrasekar (16904526)
Omar Aboumarzouk (18427923)
Ajay Vikram Singh (22501868)
Sarada Prasad Dakua (22501871)
author_role author
dc.creator.none.fl_str_mv Syed Mohammad (21075689)
Vaisali Chandrasekar (16904526)
Omar Aboumarzouk (18427923)
Ajay Vikram Singh (22501868)
Sarada Prasad Dakua (22501871)
dc.date.none.fl_str_mv 2025-05-14T12:00:00Z
dc.identifier.none.fl_str_mv 10.1109/access.2025.3566440
dc.relation.none.fl_str_mv https://figshare.com/articles/journal_contribution/Leveraging_Machine_and_Deep_Learning_Algorithms_for_hERG_Blocker_Prediction/30454286
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Biomedical and clinical sciences
Cardiovascular medicine and haematology
Pharmacology and pharmaceutical sciences
Health sciences
Health services and systems
Information and computing sciences
Machine learning
hERG
machine learning
cardiotoxicity
prediction
Fingerprint recognition
Feature extraction
Compounds
Classification algorithms
Chemicals
Training
Principal component analysis
Drugs
dc.title.none.fl_str_mv Leveraging Machine and Deep Learning Algorithms for hERG Blocker Prediction
dc.type.none.fl_str_mv Text
Journal contribution
info:eu-repo/semantics/publishedVersion
text
contribution to journal
description <p dir="ltr">The human ether-a-go-go-related (hERG) gene is crucial in enabling the regulation of repolarisation process in the heart. Some chemicals act as hERG blockers, resulting in prolonged QT intervals. Predicting the binding capability of molecules with hERG channels is expected to reduce the burden of cardiotoxicity testing in drug evaluation. The application of machine learning (ML) and deep learning (DL) models in the field of toxicity has gained burgeoning interest. The current study utilises state-of-the-art ML and DL models for predicting the hERG-blocking ability of chemical compounds using a dataset of 8337 molecules. It is noted that spatial relationships within molecules are crucial in predicting hERG blockers. While the threshold for blockers is defined as ≤10 μ M and for non-blockers, it is >10 μ M, our analysis indicates that a threshold of 60- 80 μ M provides a more accurate cut-off for non-blockers. This adjustment highlights the importance of concentration levels in reflecting the variability specific to individual interaction sites. The algorithm results show that the internal validation performance of RF, XGBoost, and MLP is strong, with AUC scores of 0.90, 0.90, and 0.87, respectively. In summary, the current study provides a machine learning framework for computation cardiotoxicity assessment by analysis of the hERG blocker concentration cut-offs using different fingerprints at multiple thresholds.</p><h2>Other Information</h2><p dir="ltr">Published in: IEEE Access<br>License: <a href="https://creativecommons.org/licenses/by/4.0/deed.en" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1109/access.2025.3566440" target="_blank">https://dx.doi.org/10.1109/access.2025.3566440</a></p>
eu_rights_str_mv openAccess
id Manara2_39883fb91e6ca3841c6f9e70780e1fcb
identifier_str_mv 10.1109/access.2025.3566440
network_acronym_str Manara2
network_name_str Manara2
oai_identifier_str oai:figshare.com:article/30454286
publishDate 2025
repository.mail.fl_str_mv
repository.name.fl_str_mv
repository_id_str
rights_invalid_str_mv CC BY 4.0
spelling Leveraging Machine and Deep Learning Algorithms for hERG Blocker PredictionSyed Mohammad (21075689)Vaisali Chandrasekar (16904526)Omar Aboumarzouk (18427923)Ajay Vikram Singh (22501868)Sarada Prasad Dakua (22501871)Biomedical and clinical sciencesCardiovascular medicine and haematologyPharmacology and pharmaceutical sciencesHealth sciencesHealth services and systemsInformation and computing sciencesMachine learninghERGmachine learningcardiotoxicitypredictionFingerprint recognitionFeature extractionCompoundsClassification algorithmsChemicalsTrainingPrincipal component analysisDrugs<p dir="ltr">The human ether-a-go-go-related (hERG) gene is crucial in enabling the regulation of repolarisation process in the heart. Some chemicals act as hERG blockers, resulting in prolonged QT intervals. Predicting the binding capability of molecules with hERG channels is expected to reduce the burden of cardiotoxicity testing in drug evaluation. The application of machine learning (ML) and deep learning (DL) models in the field of toxicity has gained burgeoning interest. The current study utilises state-of-the-art ML and DL models for predicting the hERG-blocking ability of chemical compounds using a dataset of 8337 molecules. It is noted that spatial relationships within molecules are crucial in predicting hERG blockers. While the threshold for blockers is defined as ≤10 μ M and for non-blockers, it is >10 μ M, our analysis indicates that a threshold of 60- 80 μ M provides a more accurate cut-off for non-blockers. This adjustment highlights the importance of concentration levels in reflecting the variability specific to individual interaction sites. The algorithm results show that the internal validation performance of RF, XGBoost, and MLP is strong, with AUC scores of 0.90, 0.90, and 0.87, respectively. In summary, the current study provides a machine learning framework for computation cardiotoxicity assessment by analysis of the hERG blocker concentration cut-offs using different fingerprints at multiple thresholds.</p><h2>Other Information</h2><p dir="ltr">Published in: IEEE Access<br>License: <a href="https://creativecommons.org/licenses/by/4.0/deed.en" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1109/access.2025.3566440" target="_blank">https://dx.doi.org/10.1109/access.2025.3566440</a></p>2025-05-14T12:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1109/access.2025.3566440https://figshare.com/articles/journal_contribution/Leveraging_Machine_and_Deep_Learning_Algorithms_for_hERG_Blocker_Prediction/30454286CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/304542862025-05-14T12:00:00Z
spellingShingle Leveraging Machine and Deep Learning Algorithms for hERG Blocker Prediction
Syed Mohammad (21075689)
Biomedical and clinical sciences
Cardiovascular medicine and haematology
Pharmacology and pharmaceutical sciences
Health sciences
Health services and systems
Information and computing sciences
Machine learning
hERG
machine learning
cardiotoxicity
prediction
Fingerprint recognition
Feature extraction
Compounds
Classification algorithms
Chemicals
Training
Principal component analysis
Drugs
status_str publishedVersion
title Leveraging Machine and Deep Learning Algorithms for hERG Blocker Prediction
title_full Leveraging Machine and Deep Learning Algorithms for hERG Blocker Prediction
title_fullStr Leveraging Machine and Deep Learning Algorithms for hERG Blocker Prediction
title_full_unstemmed Leveraging Machine and Deep Learning Algorithms for hERG Blocker Prediction
title_short Leveraging Machine and Deep Learning Algorithms for hERG Blocker Prediction
title_sort Leveraging Machine and Deep Learning Algorithms for hERG Blocker Prediction
topic Biomedical and clinical sciences
Cardiovascular medicine and haematology
Pharmacology and pharmaceutical sciences
Health sciences
Health services and systems
Information and computing sciences
Machine learning
hERG
machine learning
cardiotoxicity
prediction
Fingerprint recognition
Feature extraction
Compounds
Classification algorithms
Chemicals
Training
Principal component analysis
Drugs