Co-occurrence of antibiotic and metal resistance in long-term sewage sludge-amended soils: influence of application rates and pedo-climatic conditions
<p dir="ltr">Urban sewage sludge (USS) is increasingly being used as an alternative organic amendment in agriculture. Because USS originates mostly from human excreta, partially metabolized pharmaceuticals have also been considered in risk assessment studies after reuse. In this rega...
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| مؤلفون آخرون: | , , , , , , |
| منشور في: |
2022
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إضافة وسم
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| _version_ | 1864513546060038144 |
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| author | Sonia Mokni-Tlili (20487266) |
| author2 | Sarra Hechmi (21606218) Hadda-Imene Ouzari (6668957) Najet Mechergui (21606221) Manel Ghorbel (21606224) Naceur Jedidi (21606227) Abdennaceur Hassen (21606230) Helmi Hamdi (12578884) |
| author2_role | author author author author author author author |
| author_facet | Sonia Mokni-Tlili (20487266) Sarra Hechmi (21606218) Hadda-Imene Ouzari (6668957) Najet Mechergui (21606221) Manel Ghorbel (21606224) Naceur Jedidi (21606227) Abdennaceur Hassen (21606230) Helmi Hamdi (12578884) |
| author_role | author |
| dc.creator.none.fl_str_mv | Sonia Mokni-Tlili (20487266) Sarra Hechmi (21606218) Hadda-Imene Ouzari (6668957) Najet Mechergui (21606221) Manel Ghorbel (21606224) Naceur Jedidi (21606227) Abdennaceur Hassen (21606230) Helmi Hamdi (12578884) |
| dc.date.none.fl_str_mv | 2022-11-12T09:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1007/s11356-022-23802-2 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Co-occurrence_of_antibiotic_and_metal_resistance_in_long-term_sewage_sludge-amended_soils_influence_of_application_rates_and_pedo-climatic_conditions/29413343 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Environmental sciences Soil sciences Health sciences Public health Urban sewage sludge Organic amendment Agricultural soils Antibiotic-resistant bacteria Metal resistance Flash flood |
| dc.title.none.fl_str_mv | Co-occurrence of antibiotic and metal resistance in long-term sewage sludge-amended soils: influence of application rates and pedo-climatic conditions |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Urban sewage sludge (USS) is increasingly being used as an alternative organic amendment in agriculture. Because USS originates mostly from human excreta, partially metabolized pharmaceuticals have also been considered in risk assessment studies after reuse. In this regard, we investigated the cumulative effect of five annual USS applications on the spread of antibiotic-resistant bacteria (ARB) and their subsequent resistance to toxic metals in two unvegetated soils. Eventually, USS contained bacterial strains resistant to all addressed antibiotics with indices of resistance varying between 0.25 for gentamicin to 38% for ampicillin and azithromycin. Sludge-amended soils showed also the emergence of resistome for all tested antibiotics compared to non-treated controls. In this regard, the increase of sludge dose generally correlated with ARB counts, while soil texture had no influence. On the other hand, the multi-antibiotic resistance (MAR) of 52 isolates selected from USS and different soil treatments was investigated for 10 most prescribed antibiotics. Nine isolates showed significant MAR index (≥ 0.3) and co-resistance to Cd, As and Be as well. However, events including an extreme flash flood and the termination of USS applications significantly disrupted ARB communities in all soil treatments. In any case, this study highlighted the risks of ARB spread in sludge-amended soils and a greater concern with the recent exacerbation of antibiotic overuse following COVID-19 outbreak.</p><h2>Other Information</h2><p dir="ltr">Published in: Environmental Science and Pollution Research<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1007/s11356-022-23802-2" target="_blank">https://dx.doi.org/10.1007/s11356-022-23802-2</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_3d06030e19234ecaa3b512c2885ba96c |
| identifier_str_mv | 10.1007/s11356-022-23802-2 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/29413343 |
| publishDate | 2022 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Co-occurrence of antibiotic and metal resistance in long-term sewage sludge-amended soils: influence of application rates and pedo-climatic conditionsSonia Mokni-Tlili (20487266)Sarra Hechmi (21606218)Hadda-Imene Ouzari (6668957)Najet Mechergui (21606221)Manel Ghorbel (21606224)Naceur Jedidi (21606227)Abdennaceur Hassen (21606230)Helmi Hamdi (12578884)Environmental sciencesSoil sciencesHealth sciencesPublic healthUrban sewage sludgeOrganic amendmentAgricultural soilsAntibiotic-resistant bacteriaMetal resistanceFlash flood<p dir="ltr">Urban sewage sludge (USS) is increasingly being used as an alternative organic amendment in agriculture. Because USS originates mostly from human excreta, partially metabolized pharmaceuticals have also been considered in risk assessment studies after reuse. In this regard, we investigated the cumulative effect of five annual USS applications on the spread of antibiotic-resistant bacteria (ARB) and their subsequent resistance to toxic metals in two unvegetated soils. Eventually, USS contained bacterial strains resistant to all addressed antibiotics with indices of resistance varying between 0.25 for gentamicin to 38% for ampicillin and azithromycin. Sludge-amended soils showed also the emergence of resistome for all tested antibiotics compared to non-treated controls. In this regard, the increase of sludge dose generally correlated with ARB counts, while soil texture had no influence. On the other hand, the multi-antibiotic resistance (MAR) of 52 isolates selected from USS and different soil treatments was investigated for 10 most prescribed antibiotics. Nine isolates showed significant MAR index (≥ 0.3) and co-resistance to Cd, As and Be as well. However, events including an extreme flash flood and the termination of USS applications significantly disrupted ARB communities in all soil treatments. In any case, this study highlighted the risks of ARB spread in sludge-amended soils and a greater concern with the recent exacerbation of antibiotic overuse following COVID-19 outbreak.</p><h2>Other Information</h2><p dir="ltr">Published in: Environmental Science and Pollution Research<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1007/s11356-022-23802-2" target="_blank">https://dx.doi.org/10.1007/s11356-022-23802-2</a></p>2022-11-12T09:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1007/s11356-022-23802-2https://figshare.com/articles/journal_contribution/Co-occurrence_of_antibiotic_and_metal_resistance_in_long-term_sewage_sludge-amended_soils_influence_of_application_rates_and_pedo-climatic_conditions/29413343CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/294133432022-11-12T09:00:00Z |
| spellingShingle | Co-occurrence of antibiotic and metal resistance in long-term sewage sludge-amended soils: influence of application rates and pedo-climatic conditions Sonia Mokni-Tlili (20487266) Environmental sciences Soil sciences Health sciences Public health Urban sewage sludge Organic amendment Agricultural soils Antibiotic-resistant bacteria Metal resistance Flash flood |
| status_str | publishedVersion |
| title | Co-occurrence of antibiotic and metal resistance in long-term sewage sludge-amended soils: influence of application rates and pedo-climatic conditions |
| title_full | Co-occurrence of antibiotic and metal resistance in long-term sewage sludge-amended soils: influence of application rates and pedo-climatic conditions |
| title_fullStr | Co-occurrence of antibiotic and metal resistance in long-term sewage sludge-amended soils: influence of application rates and pedo-climatic conditions |
| title_full_unstemmed | Co-occurrence of antibiotic and metal resistance in long-term sewage sludge-amended soils: influence of application rates and pedo-climatic conditions |
| title_short | Co-occurrence of antibiotic and metal resistance in long-term sewage sludge-amended soils: influence of application rates and pedo-climatic conditions |
| title_sort | Co-occurrence of antibiotic and metal resistance in long-term sewage sludge-amended soils: influence of application rates and pedo-climatic conditions |
| topic | Environmental sciences Soil sciences Health sciences Public health Urban sewage sludge Organic amendment Agricultural soils Antibiotic-resistant bacteria Metal resistance Flash flood |