Transcriptome and Literature Mining Highlight the Differential Expression of ERLIN1 in Immune Cells during Sepsis
<div><p>Sepsis results from the dysregulation of the host immune system. This highly variable disease affects 19 million people globally, and accounts for 5 million deaths annually. In transcriptomic datasets curated from public repositories, we observed a consistent upregulation (3.26–5...
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2021
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| author | Susie S. Y. Huang (2824502) |
| author2 | Mohammed Toufiq (7251596) Luis R. Saraiva (18282274) Nicholas Van Panhuys (18282196) Damien Chaussabel (26369) Mathieu Garand (4861942) |
| author2_role | author author author author author |
| author_facet | Susie S. Y. Huang (2824502) Mohammed Toufiq (7251596) Luis R. Saraiva (18282274) Nicholas Van Panhuys (18282196) Damien Chaussabel (26369) Mathieu Garand (4861942) |
| author_role | author |
| dc.creator.none.fl_str_mv | Susie S. Y. Huang (2824502) Mohammed Toufiq (7251596) Luis R. Saraiva (18282274) Nicholas Van Panhuys (18282196) Damien Chaussabel (26369) Mathieu Garand (4861942) |
| dc.date.none.fl_str_mv | 2021-08-05T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3390/biology10080755 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Transcriptome_and_Literature_Mining_Highlight_the_Differential_Expression_of_ERLIN1_in_Immune_Cells_during_Sepsis/25764324 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Immunology cholesterol biosynthesis immunometabolism leukocytes calcium channel bacteremia sepsis neutrophil myeloid cells innate immunity |
| dc.title.none.fl_str_mv | Transcriptome and Literature Mining Highlight the Differential Expression of ERLIN1 in Immune Cells during Sepsis |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <div><p>Sepsis results from the dysregulation of the host immune system. This highly variable disease affects 19 million people globally, and accounts for 5 million deaths annually. In transcriptomic datasets curated from public repositories, we observed a consistent upregulation (3.26–5.29 fold) of ERLIN1—a gene coding for an ER membrane prohibitin and a regulator of inositol 1, 4, 5-trisphosphate receptors and sterol regulatory element-binding proteins—under septic conditions in healthy neutrophils, monocytes, and whole blood. In vitro expression of the ERLIN1 gene and proteins was measured by stimulating the whole blood of healthy volunteers to a combination of lipopolysaccharide and peptidoglycan. Septic stimulation induced a significant increase in ERLIN1 expression; however, ERLIN1 was differentially expressed among the immune blood cell subsets. ERLIN1 was uniquely increased in whole blood neutrophils, and confirmed in the differentiated HL60 cell line. The scarcity of ERLIN1 in sepsis literature indicates a knowledge gap between the functions of ERLIN1, calcium homeostasis, and cholesterol and fatty acid biosynthesis, and sepsis. In combination with experimental data, we bring forth the hypothesis that ERLIN1 is variably modulated among immune cells in response to cellular perturbations, and has implications for ER functions and/or ER membrane protein components during sepsis.</p><p> </p></div><h2>Other Information</h2> <p> Published in: Biology<br> License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/biology10080755" target="_blank">https://dx.doi.org/10.3390/biology10080755</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_422a1ca534f9e511e59b1e664ad477cc |
| identifier_str_mv | 10.3390/biology10080755 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25764324 |
| publishDate | 2021 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Transcriptome and Literature Mining Highlight the Differential Expression of ERLIN1 in Immune Cells during SepsisSusie S. Y. Huang (2824502)Mohammed Toufiq (7251596)Luis R. Saraiva (18282274)Nicholas Van Panhuys (18282196)Damien Chaussabel (26369)Mathieu Garand (4861942)Biomedical and clinical sciencesImmunologycholesterol biosynthesisimmunometabolismleukocytescalcium channelbacteremiasepsisneutrophilmyeloid cellsinnate immunity<div><p>Sepsis results from the dysregulation of the host immune system. This highly variable disease affects 19 million people globally, and accounts for 5 million deaths annually. In transcriptomic datasets curated from public repositories, we observed a consistent upregulation (3.26–5.29 fold) of ERLIN1—a gene coding for an ER membrane prohibitin and a regulator of inositol 1, 4, 5-trisphosphate receptors and sterol regulatory element-binding proteins—under septic conditions in healthy neutrophils, monocytes, and whole blood. In vitro expression of the ERLIN1 gene and proteins was measured by stimulating the whole blood of healthy volunteers to a combination of lipopolysaccharide and peptidoglycan. Septic stimulation induced a significant increase in ERLIN1 expression; however, ERLIN1 was differentially expressed among the immune blood cell subsets. ERLIN1 was uniquely increased in whole blood neutrophils, and confirmed in the differentiated HL60 cell line. The scarcity of ERLIN1 in sepsis literature indicates a knowledge gap between the functions of ERLIN1, calcium homeostasis, and cholesterol and fatty acid biosynthesis, and sepsis. In combination with experimental data, we bring forth the hypothesis that ERLIN1 is variably modulated among immune cells in response to cellular perturbations, and has implications for ER functions and/or ER membrane protein components during sepsis.</p><p> </p></div><h2>Other Information</h2> <p> Published in: Biology<br> License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/biology10080755" target="_blank">https://dx.doi.org/10.3390/biology10080755</a></p>2021-08-05T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3390/biology10080755https://figshare.com/articles/journal_contribution/Transcriptome_and_Literature_Mining_Highlight_the_Differential_Expression_of_ERLIN1_in_Immune_Cells_during_Sepsis/25764324CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/257643242021-08-05T03:00:00Z |
| spellingShingle | Transcriptome and Literature Mining Highlight the Differential Expression of ERLIN1 in Immune Cells during Sepsis Susie S. Y. Huang (2824502) Biomedical and clinical sciences Immunology cholesterol biosynthesis immunometabolism leukocytes calcium channel bacteremia sepsis neutrophil myeloid cells innate immunity |
| status_str | publishedVersion |
| title | Transcriptome and Literature Mining Highlight the Differential Expression of ERLIN1 in Immune Cells during Sepsis |
| title_full | Transcriptome and Literature Mining Highlight the Differential Expression of ERLIN1 in Immune Cells during Sepsis |
| title_fullStr | Transcriptome and Literature Mining Highlight the Differential Expression of ERLIN1 in Immune Cells during Sepsis |
| title_full_unstemmed | Transcriptome and Literature Mining Highlight the Differential Expression of ERLIN1 in Immune Cells during Sepsis |
| title_short | Transcriptome and Literature Mining Highlight the Differential Expression of ERLIN1 in Immune Cells during Sepsis |
| title_sort | Transcriptome and Literature Mining Highlight the Differential Expression of ERLIN1 in Immune Cells during Sepsis |
| topic | Biomedical and clinical sciences Immunology cholesterol biosynthesis immunometabolism leukocytes calcium channel bacteremia sepsis neutrophil myeloid cells innate immunity |