The prevalence of HEV among non-A-C hepatitis in Qatar and efficiency of serological markers for the diagnosis of hepatitis E
<h3>Background</h3><p dir="ltr">The rapid growth of Qatar in the last two decades has attracted a large influx of immigrant workers who mostly come from HEV-hyperendemic countries. Thus, we aim to investigate the prevalence of HEV among acute non-A-C hepatitis patients in...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , , , , , , |
| منشور في: |
2021
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| _version_ | 1864513516673695744 |
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| author | Enas S. Al Absi (10976751) |
| author2 | Duaa W. Al-Sadeq (10976754) Makiyeh Khalili (10976757) Nadin Younes (4863280) Nader Al-Dewik (4166527) Sara K. Abdelghany (10976760) Somaia S. Abouzid (10976763) Asma A. Al Thani (10976766) Hadi M. Yassine (4675846) Peter V. Coyle (6837260) Gheyath K. Nasrallah (9200525) |
| author2_role | author author author author author author author author author author |
| author_facet | Enas S. Al Absi (10976751) Duaa W. Al-Sadeq (10976754) Makiyeh Khalili (10976757) Nadin Younes (4863280) Nader Al-Dewik (4166527) Sara K. Abdelghany (10976760) Somaia S. Abouzid (10976763) Asma A. Al Thani (10976766) Hadi M. Yassine (4675846) Peter V. Coyle (6837260) Gheyath K. Nasrallah (9200525) |
| author_role | author |
| dc.creator.none.fl_str_mv | Enas S. Al Absi (10976751) Duaa W. Al-Sadeq (10976754) Makiyeh Khalili (10976757) Nadin Younes (4863280) Nader Al-Dewik (4166527) Sara K. Abdelghany (10976760) Somaia S. Abouzid (10976763) Asma A. Al Thani (10976766) Hadi M. Yassine (4675846) Peter V. Coyle (6837260) Gheyath K. Nasrallah (9200525) |
| dc.date.none.fl_str_mv | 2021-06-15T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1186/s12876-021-01841-2 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/The_prevalence_of_HEV_among_non-A-C_hepatitis_in_Qatar_and_efficiency_of_serological_markers_for_the_diagnosis_of_hepatitis_E/25771530 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Medical microbiology Non-A-C hepatitis RT-PCR Wantai ELISA Test performance Prevalence Qatar |
| dc.title.none.fl_str_mv | The prevalence of HEV among non-A-C hepatitis in Qatar and efficiency of serological markers for the diagnosis of hepatitis E |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <h3>Background</h3><p dir="ltr">The rapid growth of Qatar in the last two decades has attracted a large influx of immigrant workers who mostly come from HEV-hyperendemic countries. Thus, we aim to investigate the prevalence of HEV among acute non-A-C hepatitis patients in Qatar; and to evaluate the performance of four dominant commercial serological assays for HEV diagnosis.</p><h3>Methods</h3><p dir="ltr">259 patients with non-A-C hepatitis were tested using the Wantai HEV-IgM, HEV-IgG, HEV-Ag ELISA kits, and the MP Biomedical HEV-Total Ab ELISA kit. ALT levels were tested and HEV RNA (viral loads) was performed using Taqman AmpliCube HEV RT-PCR kit (Mikrogen, Neuried, Germany). The performance of each kit was assessed according to the RT-PCR results.</p><h3>Results</h3><p dir="ltr">HEV-RNA was detected in 23.1% of the samples. Most of these HEV-RNA-positive cases belonged to non-Qatari residents from the Indian subcontinent; India, Pakistan, etc. HEV-Ag, HEV-IgM, HEV-IgG, HEV-Total Ab were detected in 5.56%, 8.65%, 32.1%, and 34.2% of all tested samples, respectively. Elevated ALT levels were highly correlated with the HEV-Ag, HEV-IgM, HEV-RNA but not with the HEV-IgG and HEV-Total Ab. Although HEV-Ag was very specific (100%), yet its sensitivity was poor (36.7%). HEV-IgM demonstrated the best second marker for diagnosis of acute HEV after RT-PCR as jugged by the overall performance parameters: specificity (96.2%), sensitivity (71.4%), PPV (83.3%), NPP (92.7%), agreement with RT-PCR (91.0%), and Kappa-value (0.71).</p><h3>Conclusion</h3><p dir="ltr">Our study demonstrated a high prevalence of HEV virus in Qatar, mostly among immigrants from the Indian subcontinent. The HEV-IgM represents the best marker for detecting the acute HEV infection, where RT-PCR cannot be performed.</p><h2>Other Information</h2><p dir="ltr">Published in: BMC Gastroenterology<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1186/s12876-021-01841-2" target="_blank">https://dx.doi.org/10.1186/s12876-021-01841-2</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_450adf053415d74fa0eb8d74ad2f454c |
| identifier_str_mv | 10.1186/s12876-021-01841-2 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25771530 |
| publishDate | 2021 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | The prevalence of HEV among non-A-C hepatitis in Qatar and efficiency of serological markers for the diagnosis of hepatitis EEnas S. Al Absi (10976751)Duaa W. Al-Sadeq (10976754)Makiyeh Khalili (10976757)Nadin Younes (4863280)Nader Al-Dewik (4166527)Sara K. Abdelghany (10976760)Somaia S. Abouzid (10976763)Asma A. Al Thani (10976766)Hadi M. Yassine (4675846)Peter V. Coyle (6837260)Gheyath K. Nasrallah (9200525)Biomedical and clinical sciencesMedical microbiologyNon-A-C hepatitisRT-PCRWantai ELISATest performancePrevalenceQatar<h3>Background</h3><p dir="ltr">The rapid growth of Qatar in the last two decades has attracted a large influx of immigrant workers who mostly come from HEV-hyperendemic countries. Thus, we aim to investigate the prevalence of HEV among acute non-A-C hepatitis patients in Qatar; and to evaluate the performance of four dominant commercial serological assays for HEV diagnosis.</p><h3>Methods</h3><p dir="ltr">259 patients with non-A-C hepatitis were tested using the Wantai HEV-IgM, HEV-IgG, HEV-Ag ELISA kits, and the MP Biomedical HEV-Total Ab ELISA kit. ALT levels were tested and HEV RNA (viral loads) was performed using Taqman AmpliCube HEV RT-PCR kit (Mikrogen, Neuried, Germany). The performance of each kit was assessed according to the RT-PCR results.</p><h3>Results</h3><p dir="ltr">HEV-RNA was detected in 23.1% of the samples. Most of these HEV-RNA-positive cases belonged to non-Qatari residents from the Indian subcontinent; India, Pakistan, etc. HEV-Ag, HEV-IgM, HEV-IgG, HEV-Total Ab were detected in 5.56%, 8.65%, 32.1%, and 34.2% of all tested samples, respectively. Elevated ALT levels were highly correlated with the HEV-Ag, HEV-IgM, HEV-RNA but not with the HEV-IgG and HEV-Total Ab. Although HEV-Ag was very specific (100%), yet its sensitivity was poor (36.7%). HEV-IgM demonstrated the best second marker for diagnosis of acute HEV after RT-PCR as jugged by the overall performance parameters: specificity (96.2%), sensitivity (71.4%), PPV (83.3%), NPP (92.7%), agreement with RT-PCR (91.0%), and Kappa-value (0.71).</p><h3>Conclusion</h3><p dir="ltr">Our study demonstrated a high prevalence of HEV virus in Qatar, mostly among immigrants from the Indian subcontinent. The HEV-IgM represents the best marker for detecting the acute HEV infection, where RT-PCR cannot be performed.</p><h2>Other Information</h2><p dir="ltr">Published in: BMC Gastroenterology<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1186/s12876-021-01841-2" target="_blank">https://dx.doi.org/10.1186/s12876-021-01841-2</a></p>2021-06-15T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1186/s12876-021-01841-2https://figshare.com/articles/journal_contribution/The_prevalence_of_HEV_among_non-A-C_hepatitis_in_Qatar_and_efficiency_of_serological_markers_for_the_diagnosis_of_hepatitis_E/25771530CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/257715302021-06-15T03:00:00Z |
| spellingShingle | The prevalence of HEV among non-A-C hepatitis in Qatar and efficiency of serological markers for the diagnosis of hepatitis E Enas S. Al Absi (10976751) Biomedical and clinical sciences Medical microbiology Non-A-C hepatitis RT-PCR Wantai ELISA Test performance Prevalence Qatar |
| status_str | publishedVersion |
| title | The prevalence of HEV among non-A-C hepatitis in Qatar and efficiency of serological markers for the diagnosis of hepatitis E |
| title_full | The prevalence of HEV among non-A-C hepatitis in Qatar and efficiency of serological markers for the diagnosis of hepatitis E |
| title_fullStr | The prevalence of HEV among non-A-C hepatitis in Qatar and efficiency of serological markers for the diagnosis of hepatitis E |
| title_full_unstemmed | The prevalence of HEV among non-A-C hepatitis in Qatar and efficiency of serological markers for the diagnosis of hepatitis E |
| title_short | The prevalence of HEV among non-A-C hepatitis in Qatar and efficiency of serological markers for the diagnosis of hepatitis E |
| title_sort | The prevalence of HEV among non-A-C hepatitis in Qatar and efficiency of serological markers for the diagnosis of hepatitis E |
| topic | Biomedical and clinical sciences Medical microbiology Non-A-C hepatitis RT-PCR Wantai ELISA Test performance Prevalence Qatar |