Discovery of a novel cytokine signature for the diagnosis of autism spectrum disorder in young Arab children in Qatar
<h3>Background</h3><p dir="ltr">Autism spectrum disorder (ASD) is a heterogeneous neurodevelopmental disorder characterized by impaired social interaction and communication and the occurrence of stereotyped and repetitive behaviors. Several studies have reported altered c...
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| مؤلفون آخرون: | , , , , , |
| منشور في: |
2024
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إضافة وسم
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| _version_ | 1864513510180913152 |
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| author | Wared Nour-Eldine (13904298) |
| author2 | Nimshitha Pavathuparambil Abdul Manaph (17953718) Samia M. Ltaief (11405336) Nazim Abdel Aati (18614887) Monaa Hussain Mansoori (17953721) Samya Al Abdulla (16442153) Abeer R. Al-Shammari (11405339) |
| author2_role | author author author author author author |
| author_facet | Wared Nour-Eldine (13904298) Nimshitha Pavathuparambil Abdul Manaph (17953718) Samia M. Ltaief (11405336) Nazim Abdel Aati (18614887) Monaa Hussain Mansoori (17953721) Samya Al Abdulla (16442153) Abeer R. Al-Shammari (11405339) |
| author_role | author |
| dc.creator.none.fl_str_mv | Wared Nour-Eldine (13904298) Nimshitha Pavathuparambil Abdul Manaph (17953718) Samia M. Ltaief (11405336) Nazim Abdel Aati (18614887) Monaa Hussain Mansoori (17953721) Samya Al Abdulla (16442153) Abeer R. Al-Shammari (11405339) |
| dc.date.none.fl_str_mv | 2024-02-13T09:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3389/fpsyt.2024.1333534 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Discovery_of_a_novel_cytokine_signature_for_the_diagnosis_of_autism_spectrum_disorder_in_young_Arab_children_in_Qatar/26392843 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Clinical sciences Neurosciences Paediatrics autism spectrum disorder ASD cytokines biomarkers luminex multiplex assay logistic regression |
| dc.title.none.fl_str_mv | Discovery of a novel cytokine signature for the diagnosis of autism spectrum disorder in young Arab children in Qatar |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <h3>Background</h3><p dir="ltr">Autism spectrum disorder (ASD) is a heterogeneous neurodevelopmental disorder characterized by impaired social interaction and communication and the occurrence of stereotyped and repetitive behaviors. Several studies have reported altered cytokine profiles in ASD and hence may serve as potential diagnostic biomarkers of the disorder. This study aims to identify diagnostic biomarkers for ASD in a well-defined study cohort in Qatar.</p><h3>Methods</h3><p dir="ltr">We measured the protein levels of 45 cytokines in the plasma samples of age- and gender-matched children (2–4 years) with ASD (n = 100) and controls (n = 60) using a Luminex multiplex assay. We compared the differences in the levels of these cytokines between the two study groups and then fitted the significantly altered cytokines into a logistic regression model to examine their diagnostic potential for ASD.</p><h3>Results</h3><p dir="ltr">We found elevated levels of IFN-γ, FGF-2, IL-1RA, and IL-13 and reduced levels of eotaxin, HGF, IL-1 alpha, IL-22, IL-9, MCP-1, SCF, SDF-1 alpha, VEGFA, and IP-10 in the plasma of children with ASD compared to controls. Furthermore, we observed that elevated levels of IFN-γ (odds ratio (OR) = 1.823; 95% (confidence interval) CI = 1.206, 2.755; p = 0.004) and FGF-2 (OR = 2.528; 95% CI = 1.457, 4.385; p < 0.001) were significantly associated with increased odds of ASD, whereas reduced levels of eotaxin (OR = 0.350; 95% CI = 0.160, 0.765; p = 0.008) and HGF (OR = 0.220; 95% CI = 0.070, 0.696; p = 0.010) were significantly associated with lower odds of ASD relative to controls. The combination of these four cytokines revealed an area under the curve (ROC-AUC) of 0.829 (95% CI = 0.767, 0.891; p < 0.001), which demonstrates the diagnostic accuracy of the four-cytokine signature.</p><h3>Conclusions</h3><p dir="ltr">Our results identified a panel of cytokines that could discriminate between children with ASD and controls in Qatar. In addition, our findings support the predominance of a Th1 immune phenotype in ASD children and emphasize the need to validate these results in larger populations.</p><h2>Other Information</h2><p dir="ltr">Published in: Frontiers in Psychiatry<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3389/fpsyt.2024.1333534" target="_blank">https://dx.doi.org/10.3389/fpsyt.2024.1333534</a></p><p dir="ltr">Additional institutions affiliated with: Child Development Center - Rumailah Hospital, Primary Health Care Corporation - Al-Wajbah Health Center</p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_47403a030509a8f4a4fc1f3fd4894b32 |
| identifier_str_mv | 10.3389/fpsyt.2024.1333534 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/26392843 |
| publishDate | 2024 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Discovery of a novel cytokine signature for the diagnosis of autism spectrum disorder in young Arab children in QatarWared Nour-Eldine (13904298)Nimshitha Pavathuparambil Abdul Manaph (17953718)Samia M. Ltaief (11405336)Nazim Abdel Aati (18614887)Monaa Hussain Mansoori (17953721)Samya Al Abdulla (16442153)Abeer R. Al-Shammari (11405339)Biomedical and clinical sciencesClinical sciencesNeurosciencesPaediatricsautism spectrum disorderASDcytokinesbiomarkersluminex multiplex assaylogistic regression<h3>Background</h3><p dir="ltr">Autism spectrum disorder (ASD) is a heterogeneous neurodevelopmental disorder characterized by impaired social interaction and communication and the occurrence of stereotyped and repetitive behaviors. Several studies have reported altered cytokine profiles in ASD and hence may serve as potential diagnostic biomarkers of the disorder. This study aims to identify diagnostic biomarkers for ASD in a well-defined study cohort in Qatar.</p><h3>Methods</h3><p dir="ltr">We measured the protein levels of 45 cytokines in the plasma samples of age- and gender-matched children (2–4 years) with ASD (n = 100) and controls (n = 60) using a Luminex multiplex assay. We compared the differences in the levels of these cytokines between the two study groups and then fitted the significantly altered cytokines into a logistic regression model to examine their diagnostic potential for ASD.</p><h3>Results</h3><p dir="ltr">We found elevated levels of IFN-γ, FGF-2, IL-1RA, and IL-13 and reduced levels of eotaxin, HGF, IL-1 alpha, IL-22, IL-9, MCP-1, SCF, SDF-1 alpha, VEGFA, and IP-10 in the plasma of children with ASD compared to controls. Furthermore, we observed that elevated levels of IFN-γ (odds ratio (OR) = 1.823; 95% (confidence interval) CI = 1.206, 2.755; p = 0.004) and FGF-2 (OR = 2.528; 95% CI = 1.457, 4.385; p < 0.001) were significantly associated with increased odds of ASD, whereas reduced levels of eotaxin (OR = 0.350; 95% CI = 0.160, 0.765; p = 0.008) and HGF (OR = 0.220; 95% CI = 0.070, 0.696; p = 0.010) were significantly associated with lower odds of ASD relative to controls. The combination of these four cytokines revealed an area under the curve (ROC-AUC) of 0.829 (95% CI = 0.767, 0.891; p < 0.001), which demonstrates the diagnostic accuracy of the four-cytokine signature.</p><h3>Conclusions</h3><p dir="ltr">Our results identified a panel of cytokines that could discriminate between children with ASD and controls in Qatar. In addition, our findings support the predominance of a Th1 immune phenotype in ASD children and emphasize the need to validate these results in larger populations.</p><h2>Other Information</h2><p dir="ltr">Published in: Frontiers in Psychiatry<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3389/fpsyt.2024.1333534" target="_blank">https://dx.doi.org/10.3389/fpsyt.2024.1333534</a></p><p dir="ltr">Additional institutions affiliated with: Child Development Center - Rumailah Hospital, Primary Health Care Corporation - Al-Wajbah Health Center</p>2024-02-13T09:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3389/fpsyt.2024.1333534https://figshare.com/articles/journal_contribution/Discovery_of_a_novel_cytokine_signature_for_the_diagnosis_of_autism_spectrum_disorder_in_young_Arab_children_in_Qatar/26392843CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/263928432024-02-13T09:00:00Z |
| spellingShingle | Discovery of a novel cytokine signature for the diagnosis of autism spectrum disorder in young Arab children in Qatar Wared Nour-Eldine (13904298) Biomedical and clinical sciences Clinical sciences Neurosciences Paediatrics autism spectrum disorder ASD cytokines biomarkers luminex multiplex assay logistic regression |
| status_str | publishedVersion |
| title | Discovery of a novel cytokine signature for the diagnosis of autism spectrum disorder in young Arab children in Qatar |
| title_full | Discovery of a novel cytokine signature for the diagnosis of autism spectrum disorder in young Arab children in Qatar |
| title_fullStr | Discovery of a novel cytokine signature for the diagnosis of autism spectrum disorder in young Arab children in Qatar |
| title_full_unstemmed | Discovery of a novel cytokine signature for the diagnosis of autism spectrum disorder in young Arab children in Qatar |
| title_short | Discovery of a novel cytokine signature for the diagnosis of autism spectrum disorder in young Arab children in Qatar |
| title_sort | Discovery of a novel cytokine signature for the diagnosis of autism spectrum disorder in young Arab children in Qatar |
| topic | Biomedical and clinical sciences Clinical sciences Neurosciences Paediatrics autism spectrum disorder ASD cytokines biomarkers luminex multiplex assay logistic regression |