Pyocin QDD1: A highly thermostable bacteriocin produced by Pseudomonas aeruginosa QDD1 for the biocontrol of foodborne pathogens Staphylococcus aureus and Bacillus cereus

<p>This study was designed to explore alternative antibacterial bioresource products, bacteriocins produced by local strain of Pseudomonas aeruginosa QDD1. This bacteriocin (termed as Pyocin) has high inhibitory activity against Gram-positive bacteria including S. aureus, B. cereus, B. thuring...

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محفوظ في:
التفاصيل البيبلوغرافية
المؤلف الرئيسي: Minoli Nitin Doshi (17562639) (author)
مؤلفون آخرون: Kavita Nair (17151127) (author), Zahoor Ul Hassan (9759642) (author), Samir Jaoua (11624805) (author)
منشور في: 2022
الموضوعات:
الوسوم: إضافة وسم
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author Minoli Nitin Doshi (17562639)
author2 Kavita Nair (17151127)
Zahoor Ul Hassan (9759642)
Samir Jaoua (11624805)
author2_role author
author
author
author_facet Minoli Nitin Doshi (17562639)
Kavita Nair (17151127)
Zahoor Ul Hassan (9759642)
Samir Jaoua (11624805)
author_role author
dc.creator.none.fl_str_mv Minoli Nitin Doshi (17562639)
Kavita Nair (17151127)
Zahoor Ul Hassan (9759642)
Samir Jaoua (11624805)
dc.date.none.fl_str_mv 2022-06-01T00:00:00Z
dc.identifier.none.fl_str_mv 10.1016/j.biteb.2022.101106
dc.relation.none.fl_str_mv https://figshare.com/articles/journal_contribution/Pyocin_QDD1_A_highly_thermostable_bacteriocin_produced_by_Pseudomonas_aeruginosa_QDD1_for_the_biocontrol_of_foodborne_pathogens_Staphylococcus_aureus_and_Bacillus_cereus/24745104
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Agricultural, veterinary and food sciences
Food sciences
Biological sciences
Industrial biotechnology
Microbiology
Pseudomonas aeruginosa
Food safety
Bacteriocin
Antimicrobial activity
Thermostability
dc.title.none.fl_str_mv Pyocin QDD1: A highly thermostable bacteriocin produced by Pseudomonas aeruginosa QDD1 for the biocontrol of foodborne pathogens Staphylococcus aureus and Bacillus cereus
dc.type.none.fl_str_mv Text
Journal contribution
info:eu-repo/semantics/publishedVersion
text
contribution to journal
description <p>This study was designed to explore alternative antibacterial bioresource products, bacteriocins produced by local strain of Pseudomonas aeruginosa QDD1. This bacteriocin (termed as Pyocin) has high inhibitory activity against Gram-positive bacteria including S. aureus, B. cereus, B. thuringiensis and B. subtilis. QDD1 Pyocin production was constitutive, auto-regulated and reached an activity level of 110 AU/mL. It affects 40 % of sensitive cells starting from the 1 st hour. Pyocin QDD1 activity was not affected by Proteinase K, α-amylase, β-mercaptoethanol and 8 M urea. It displayed broad pH stability (pH 3–9) and significant thermostability (121 °C). Considering that the spores produced by Bacillus cereus are highly resistant to cooking temperatures and cause food poisoning upon germination in gastrointestinal tract, Pyocin QDD1's ability to withstand high temperatures can help control Bacillus cereus populations in contaminated food products. Thus, Pyocin QDD1 can be considered as an important bioresource product of industrial applications.</p><h2>Other Information</h2> <p> Published in: Bioresource Technology Reports<br> License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.biteb.2022.101106" target="_blank">https://dx.doi.org/10.1016/j.biteb.2022.101106</a></p>
eu_rights_str_mv openAccess
id Manara2_493266dd07431da465aaf7c14b766d64
identifier_str_mv 10.1016/j.biteb.2022.101106
network_acronym_str Manara2
network_name_str Manara2
oai_identifier_str oai:figshare.com:article/24745104
publishDate 2022
repository.mail.fl_str_mv
repository.name.fl_str_mv
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rights_invalid_str_mv CC BY 4.0
spelling Pyocin QDD1: A highly thermostable bacteriocin produced by Pseudomonas aeruginosa QDD1 for the biocontrol of foodborne pathogens Staphylococcus aureus and Bacillus cereusMinoli Nitin Doshi (17562639)Kavita Nair (17151127)Zahoor Ul Hassan (9759642)Samir Jaoua (11624805)Agricultural, veterinary and food sciencesFood sciencesBiological sciencesIndustrial biotechnologyMicrobiologyPseudomonas aeruginosaFood safetyBacteriocinAntimicrobial activityThermostability<p>This study was designed to explore alternative antibacterial bioresource products, bacteriocins produced by local strain of Pseudomonas aeruginosa QDD1. This bacteriocin (termed as Pyocin) has high inhibitory activity against Gram-positive bacteria including S. aureus, B. cereus, B. thuringiensis and B. subtilis. QDD1 Pyocin production was constitutive, auto-regulated and reached an activity level of 110 AU/mL. It affects 40 % of sensitive cells starting from the 1 st hour. Pyocin QDD1 activity was not affected by Proteinase K, α-amylase, β-mercaptoethanol and 8 M urea. It displayed broad pH stability (pH 3–9) and significant thermostability (121 °C). Considering that the spores produced by Bacillus cereus are highly resistant to cooking temperatures and cause food poisoning upon germination in gastrointestinal tract, Pyocin QDD1's ability to withstand high temperatures can help control Bacillus cereus populations in contaminated food products. Thus, Pyocin QDD1 can be considered as an important bioresource product of industrial applications.</p><h2>Other Information</h2> <p> Published in: Bioresource Technology Reports<br> License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.biteb.2022.101106" target="_blank">https://dx.doi.org/10.1016/j.biteb.2022.101106</a></p>2022-06-01T00:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1016/j.biteb.2022.101106https://figshare.com/articles/journal_contribution/Pyocin_QDD1_A_highly_thermostable_bacteriocin_produced_by_Pseudomonas_aeruginosa_QDD1_for_the_biocontrol_of_foodborne_pathogens_Staphylococcus_aureus_and_Bacillus_cereus/24745104CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/247451042022-06-01T00:00:00Z
spellingShingle Pyocin QDD1: A highly thermostable bacteriocin produced by Pseudomonas aeruginosa QDD1 for the biocontrol of foodborne pathogens Staphylococcus aureus and Bacillus cereus
Minoli Nitin Doshi (17562639)
Agricultural, veterinary and food sciences
Food sciences
Biological sciences
Industrial biotechnology
Microbiology
Pseudomonas aeruginosa
Food safety
Bacteriocin
Antimicrobial activity
Thermostability
status_str publishedVersion
title Pyocin QDD1: A highly thermostable bacteriocin produced by Pseudomonas aeruginosa QDD1 for the biocontrol of foodborne pathogens Staphylococcus aureus and Bacillus cereus
title_full Pyocin QDD1: A highly thermostable bacteriocin produced by Pseudomonas aeruginosa QDD1 for the biocontrol of foodborne pathogens Staphylococcus aureus and Bacillus cereus
title_fullStr Pyocin QDD1: A highly thermostable bacteriocin produced by Pseudomonas aeruginosa QDD1 for the biocontrol of foodborne pathogens Staphylococcus aureus and Bacillus cereus
title_full_unstemmed Pyocin QDD1: A highly thermostable bacteriocin produced by Pseudomonas aeruginosa QDD1 for the biocontrol of foodborne pathogens Staphylococcus aureus and Bacillus cereus
title_short Pyocin QDD1: A highly thermostable bacteriocin produced by Pseudomonas aeruginosa QDD1 for the biocontrol of foodborne pathogens Staphylococcus aureus and Bacillus cereus
title_sort Pyocin QDD1: A highly thermostable bacteriocin produced by Pseudomonas aeruginosa QDD1 for the biocontrol of foodborne pathogens Staphylococcus aureus and Bacillus cereus
topic Agricultural, veterinary and food sciences
Food sciences
Biological sciences
Industrial biotechnology
Microbiology
Pseudomonas aeruginosa
Food safety
Bacteriocin
Antimicrobial activity
Thermostability