Potential for native hydrocarbon-degrading bacteria to remediate highly weathered oil-polluted soils in Qatar through self-purification and bioaugmentation in biopiles

<p dir="ltr">Petroleum-derived contamination events constitute a unique environmental issue in the arid areas because of the weathering processes, accentuated due to harsh conditions and representing the main origin of failure of bioremediation applications. The industrial area of Du...

وصف كامل

محفوظ في:
التفاصيل البيبلوغرافية
المؤلف الرئيسي: Nasser AlKaabi (17075284) (author)
مؤلفون آخرون: Mohammad A. Al-Ghouti (8882054) (author), Samir Jaoua (11624805) (author), Nabil Zouari (9193418) (author)
منشور في: 2020
الموضوعات:
الوسوم: إضافة وسم
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author Nasser AlKaabi (17075284)
author2 Mohammad A. Al-Ghouti (8882054)
Samir Jaoua (11624805)
Nabil Zouari (9193418)
author2_role author
author
author
author_facet Nasser AlKaabi (17075284)
Mohammad A. Al-Ghouti (8882054)
Samir Jaoua (11624805)
Nabil Zouari (9193418)
author_role author
dc.creator.none.fl_str_mv Nasser AlKaabi (17075284)
Mohammad A. Al-Ghouti (8882054)
Samir Jaoua (11624805)
Nabil Zouari (9193418)
dc.date.none.fl_str_mv 2020-10-12T00:00:00Z
dc.identifier.none.fl_str_mv 10.1016/j.btre.2020.e00543
dc.relation.none.fl_str_mv https://figshare.com/articles/journal_contribution/Potential_for_native_hydrocarbon-degrading_bacteria_to_remediate_highly_weathered_oil-polluted_soils_in_Qatar_through_self-purification_and_bioaugmentation_in_biopiles/24225679
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Biological sciences
Ecology
Industrial biotechnology
Environmental sciences
Pollution and contamination
Weathered hydrocarbons
Bioremediation
Biostimulation
Bioaugmentation
Bacillus licheniformis
Pseudomonas aeruginosa
dc.title.none.fl_str_mv Potential for native hydrocarbon-degrading bacteria to remediate highly weathered oil-polluted soils in Qatar through self-purification and bioaugmentation in biopiles
dc.type.none.fl_str_mv Text
Journal contribution
info:eu-repo/semantics/publishedVersion
text
contribution to journal
description <p dir="ltr">Petroleum-derived contamination events constitute a unique environmental issue in the arid areas because of the weathering processes, accentuated due to harsh conditions and representing the main origin of failure of bioremediation applications. The industrial area of Dukhan and the AlZubara coast represent wethered oily-sites as an appropriate model due to the extreme weather, which characterizes Qatar. Indigenous bacteria would be highly adapted and when re-introduced or stimulated would conduct to the remediation of these sites. An appropriate approach to bioremediating-weathered oil was investigated in these two areas. Systematic soil sampling was performed, and the soil samples were analyzed. The results clearly showed the harsh chemical compositions (high salinity and contents of total petroleum components contents in the range of the oil organics). By implementation of a procedure of isolation of highly adapted bacteria, few hydrocarbon-degrading bacteria were isolated in the objective of selecting those for further application. Their identification by ribotyping showed dominance of <i>Bacillus</i> and <i>Virgibacillus</i> in AlZubara site, and <i>Bacillus</i> and <i>Pseudomonas</i> in Dukhan site. All bacterial strains were highly tolerant to 10 % diesel and showed potential of removal of 20 %–85 % of C<sub><em>10</em></sub> to C<sub><em>36</em></sub> hydrocarbons. This potential was confirmed by determination of the <i>n-</i>heptadecane/pristane and <i>n-</i>octadecane/phytane ratios, indicating in turn their potential to bioremediate oil. This was demonstrated by comparison of the self-remediation to bioaugmentation using two selected <i>Bacillus</i> and <i>Pseudomonas</i> strains using Dukhan soil in biopiles. These strains when biostimulted in such a soil increased the removal of total petroleum components to 53 % compared to 30 % with self-purification, after 90 d. The results indicated that highly adapted endogenous bacteria might be used to bioremediate highly weathered oil-contaminated soil under harsh conditions.</p><h2>Other Information</h2><p dir="ltr">Published in: Biotechnology Reports<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.btre.2020.e00543" target="_blank">https://dx.doi.org/10.1016/j.btre.2020.e00543</a></p>
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network_acronym_str Manara2
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oai_identifier_str oai:figshare.com:article/24225679
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spelling Potential for native hydrocarbon-degrading bacteria to remediate highly weathered oil-polluted soils in Qatar through self-purification and bioaugmentation in biopilesNasser AlKaabi (17075284)Mohammad A. Al-Ghouti (8882054)Samir Jaoua (11624805)Nabil Zouari (9193418)Biological sciencesEcologyIndustrial biotechnologyEnvironmental sciencesPollution and contaminationWeathered hydrocarbonsBioremediationBiostimulationBioaugmentationBacillus licheniformisPseudomonas aeruginosa<p dir="ltr">Petroleum-derived contamination events constitute a unique environmental issue in the arid areas because of the weathering processes, accentuated due to harsh conditions and representing the main origin of failure of bioremediation applications. The industrial area of Dukhan and the AlZubara coast represent wethered oily-sites as an appropriate model due to the extreme weather, which characterizes Qatar. Indigenous bacteria would be highly adapted and when re-introduced or stimulated would conduct to the remediation of these sites. An appropriate approach to bioremediating-weathered oil was investigated in these two areas. Systematic soil sampling was performed, and the soil samples were analyzed. The results clearly showed the harsh chemical compositions (high salinity and contents of total petroleum components contents in the range of the oil organics). By implementation of a procedure of isolation of highly adapted bacteria, few hydrocarbon-degrading bacteria were isolated in the objective of selecting those for further application. Their identification by ribotyping showed dominance of <i>Bacillus</i> and <i>Virgibacillus</i> in AlZubara site, and <i>Bacillus</i> and <i>Pseudomonas</i> in Dukhan site. All bacterial strains were highly tolerant to 10 % diesel and showed potential of removal of 20 %–85 % of C<sub><em>10</em></sub> to C<sub><em>36</em></sub> hydrocarbons. This potential was confirmed by determination of the <i>n-</i>heptadecane/pristane and <i>n-</i>octadecane/phytane ratios, indicating in turn their potential to bioremediate oil. This was demonstrated by comparison of the self-remediation to bioaugmentation using two selected <i>Bacillus</i> and <i>Pseudomonas</i> strains using Dukhan soil in biopiles. These strains when biostimulted in such a soil increased the removal of total petroleum components to 53 % compared to 30 % with self-purification, after 90 d. The results indicated that highly adapted endogenous bacteria might be used to bioremediate highly weathered oil-contaminated soil under harsh conditions.</p><h2>Other Information</h2><p dir="ltr">Published in: Biotechnology Reports<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.btre.2020.e00543" target="_blank">https://dx.doi.org/10.1016/j.btre.2020.e00543</a></p>2020-10-12T00:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1016/j.btre.2020.e00543https://figshare.com/articles/journal_contribution/Potential_for_native_hydrocarbon-degrading_bacteria_to_remediate_highly_weathered_oil-polluted_soils_in_Qatar_through_self-purification_and_bioaugmentation_in_biopiles/24225679CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/242256792020-10-12T00:00:00Z
spellingShingle Potential for native hydrocarbon-degrading bacteria to remediate highly weathered oil-polluted soils in Qatar through self-purification and bioaugmentation in biopiles
Nasser AlKaabi (17075284)
Biological sciences
Ecology
Industrial biotechnology
Environmental sciences
Pollution and contamination
Weathered hydrocarbons
Bioremediation
Biostimulation
Bioaugmentation
Bacillus licheniformis
Pseudomonas aeruginosa
status_str publishedVersion
title Potential for native hydrocarbon-degrading bacteria to remediate highly weathered oil-polluted soils in Qatar through self-purification and bioaugmentation in biopiles
title_full Potential for native hydrocarbon-degrading bacteria to remediate highly weathered oil-polluted soils in Qatar through self-purification and bioaugmentation in biopiles
title_fullStr Potential for native hydrocarbon-degrading bacteria to remediate highly weathered oil-polluted soils in Qatar through self-purification and bioaugmentation in biopiles
title_full_unstemmed Potential for native hydrocarbon-degrading bacteria to remediate highly weathered oil-polluted soils in Qatar through self-purification and bioaugmentation in biopiles
title_short Potential for native hydrocarbon-degrading bacteria to remediate highly weathered oil-polluted soils in Qatar through self-purification and bioaugmentation in biopiles
title_sort Potential for native hydrocarbon-degrading bacteria to remediate highly weathered oil-polluted soils in Qatar through self-purification and bioaugmentation in biopiles
topic Biological sciences
Ecology
Industrial biotechnology
Environmental sciences
Pollution and contamination
Weathered hydrocarbons
Bioremediation
Biostimulation
Bioaugmentation
Bacillus licheniformis
Pseudomonas aeruginosa