Intratumoral FoxP3<sup>+</sup>Helios<sup>+</sup> Regulatory T Cells Upregulating Immunosuppressive Molecules Are Expanded in Human Colorectal Cancer
<p dir="ltr">Regulatory T cells (Tregs) can be antitumorigenic or pro-tumorigenic in colorectal cancer (CRC) depending on the presence of different Treg subsets with various immunosuppressive molecules. Some studies reported the phenotypic characteristics of tumor-infiltrating immune...
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| مؤلفون آخرون: | , , , |
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2017
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| _version_ | 1864513557065891840 |
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| author | Azharuddin Sajid Syed Khaja (19720114) |
| author2 | Salman M. Toor (8854751) Haytham El Salhat (5396387) Bassam R. Ali (8711034) Eyad Elkord (5396390) |
| author2_role | author author author author |
| author_facet | Azharuddin Sajid Syed Khaja (19720114) Salman M. Toor (8854751) Haytham El Salhat (5396387) Bassam R. Ali (8711034) Eyad Elkord (5396390) |
| author_role | author |
| dc.creator.none.fl_str_mv | Azharuddin Sajid Syed Khaja (19720114) Salman M. Toor (8854751) Haytham El Salhat (5396387) Bassam R. Ali (8711034) Eyad Elkord (5396390) |
| dc.date.none.fl_str_mv | 2017-05-26T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3389/fimmu.2017.00619 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Intratumoral_FoxP3_sup_sup_Helios_sup_sup_Regulatory_T_Cells_Upregulating_Immunosuppressive_Molecules_Are_Expanded_in_Human_Colorectal_Cancer/27082750 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Immunology Oncology and carcinogenesis colorectal cancer regulatory T cells Forkhead box protein Helios immune checkpoint receptors tumor microenvironment |
| dc.title.none.fl_str_mv | Intratumoral FoxP3<sup>+</sup>Helios<sup>+</sup> Regulatory T Cells Upregulating Immunosuppressive Molecules Are Expanded in Human Colorectal Cancer |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Regulatory T cells (Tregs) can be antitumorigenic or pro-tumorigenic in colorectal cancer (CRC) depending on the presence of different Treg subsets with various immunosuppressive molecules. Some studies reported the phenotypic characteristics of tumor-infiltrating immune cells in CRC, but limited studies have focused on the co-expression of suppressive molecules on immune cells. The aim of this study was to characterize immune cells in the tumor microenvironment (TME), compared to paired adjacent non-tumor colon tissue of CRC patients. Additionally, we investigated co-expression of immunosuppressive molecules on different Treg subsets in the TME, normal colon tissue, and peripheral blood of CRC patients and healthy donors. In this preliminary study, we report that the majority of CD3<sup>+</sup> T cells in the TME are CD4<sup>+</sup> T cells with high co-expression of programmed death 1 (PD-1)/cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) and PD-1/CD39 molecules. Levels of CD4<sup>+</sup>FoxP3<sup>+</sup>Helios<sup>+</sup> Tregs were significantly increased in the TME. Furthermore, we observed increased levels of PD-1/CTLA-4 and PD-1/CD39 co-expressing cells within FoxP3<sup>+</sup>Helios<sup>+</sup> and FoxP3<sup>+</sup>Helios<sup>−</sup> Treg subsets, indicative of their potent immunosuppressive potential. These results suggest synergistic associations between PD-1/CTLA-4 and PD-1/CD39 in dampening T-cell activation and function along with suppressing tumor-specific immune responses, suggesting that dual blockade of these molecules could be a more effective strategy for inducing antitumor immune responses in CRC.</p><h2>Other Information</h2><p dir="ltr">Published in: Frontiers in Immunology<br>License: <a href="https://creativecommons.org/licenses/by/4.0/deed.en" rel="noreferrer noopener" target="_blank">https://creativecommons.org/licenses/by/4.0/</a> <br>See article on publisher's website: <a href="https://dx.doi.org/10.3389/fimmu.2017.00619" target="_blank">https://dx.doi.org/10.3389/fimmu.2017.00619</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_4af6dcaca198166839092cbb3aa1fa7a |
| identifier_str_mv | 10.3389/fimmu.2017.00619 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/27082750 |
| publishDate | 2017 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Intratumoral FoxP3<sup>+</sup>Helios<sup>+</sup> Regulatory T Cells Upregulating Immunosuppressive Molecules Are Expanded in Human Colorectal CancerAzharuddin Sajid Syed Khaja (19720114)Salman M. Toor (8854751)Haytham El Salhat (5396387)Bassam R. Ali (8711034)Eyad Elkord (5396390)Biomedical and clinical sciencesImmunologyOncology and carcinogenesiscolorectal cancerregulatory T cellsForkhead box proteinHeliosimmune checkpoint receptorstumor microenvironment<p dir="ltr">Regulatory T cells (Tregs) can be antitumorigenic or pro-tumorigenic in colorectal cancer (CRC) depending on the presence of different Treg subsets with various immunosuppressive molecules. Some studies reported the phenotypic characteristics of tumor-infiltrating immune cells in CRC, but limited studies have focused on the co-expression of suppressive molecules on immune cells. The aim of this study was to characterize immune cells in the tumor microenvironment (TME), compared to paired adjacent non-tumor colon tissue of CRC patients. Additionally, we investigated co-expression of immunosuppressive molecules on different Treg subsets in the TME, normal colon tissue, and peripheral blood of CRC patients and healthy donors. In this preliminary study, we report that the majority of CD3<sup>+</sup> T cells in the TME are CD4<sup>+</sup> T cells with high co-expression of programmed death 1 (PD-1)/cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) and PD-1/CD39 molecules. Levels of CD4<sup>+</sup>FoxP3<sup>+</sup>Helios<sup>+</sup> Tregs were significantly increased in the TME. Furthermore, we observed increased levels of PD-1/CTLA-4 and PD-1/CD39 co-expressing cells within FoxP3<sup>+</sup>Helios<sup>+</sup> and FoxP3<sup>+</sup>Helios<sup>−</sup> Treg subsets, indicative of their potent immunosuppressive potential. These results suggest synergistic associations between PD-1/CTLA-4 and PD-1/CD39 in dampening T-cell activation and function along with suppressing tumor-specific immune responses, suggesting that dual blockade of these molecules could be a more effective strategy for inducing antitumor immune responses in CRC.</p><h2>Other Information</h2><p dir="ltr">Published in: Frontiers in Immunology<br>License: <a href="https://creativecommons.org/licenses/by/4.0/deed.en" rel="noreferrer noopener" target="_blank">https://creativecommons.org/licenses/by/4.0/</a> <br>See article on publisher's website: <a href="https://dx.doi.org/10.3389/fimmu.2017.00619" target="_blank">https://dx.doi.org/10.3389/fimmu.2017.00619</a></p>2017-05-26T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3389/fimmu.2017.00619https://figshare.com/articles/journal_contribution/Intratumoral_FoxP3_sup_sup_Helios_sup_sup_Regulatory_T_Cells_Upregulating_Immunosuppressive_Molecules_Are_Expanded_in_Human_Colorectal_Cancer/27082750CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/270827502017-05-26T03:00:00Z |
| spellingShingle | Intratumoral FoxP3<sup>+</sup>Helios<sup>+</sup> Regulatory T Cells Upregulating Immunosuppressive Molecules Are Expanded in Human Colorectal Cancer Azharuddin Sajid Syed Khaja (19720114) Biomedical and clinical sciences Immunology Oncology and carcinogenesis colorectal cancer regulatory T cells Forkhead box protein Helios immune checkpoint receptors tumor microenvironment |
| status_str | publishedVersion |
| title | Intratumoral FoxP3<sup>+</sup>Helios<sup>+</sup> Regulatory T Cells Upregulating Immunosuppressive Molecules Are Expanded in Human Colorectal Cancer |
| title_full | Intratumoral FoxP3<sup>+</sup>Helios<sup>+</sup> Regulatory T Cells Upregulating Immunosuppressive Molecules Are Expanded in Human Colorectal Cancer |
| title_fullStr | Intratumoral FoxP3<sup>+</sup>Helios<sup>+</sup> Regulatory T Cells Upregulating Immunosuppressive Molecules Are Expanded in Human Colorectal Cancer |
| title_full_unstemmed | Intratumoral FoxP3<sup>+</sup>Helios<sup>+</sup> Regulatory T Cells Upregulating Immunosuppressive Molecules Are Expanded in Human Colorectal Cancer |
| title_short | Intratumoral FoxP3<sup>+</sup>Helios<sup>+</sup> Regulatory T Cells Upregulating Immunosuppressive Molecules Are Expanded in Human Colorectal Cancer |
| title_sort | Intratumoral FoxP3<sup>+</sup>Helios<sup>+</sup> Regulatory T Cells Upregulating Immunosuppressive Molecules Are Expanded in Human Colorectal Cancer |
| topic | Biomedical and clinical sciences Immunology Oncology and carcinogenesis colorectal cancer regulatory T cells Forkhead box protein Helios immune checkpoint receptors tumor microenvironment |