Molecular and biological characterization of influenza A viruses isolated from human fecal samples
<p dir="ltr">Human influenza viruses are occasionally detected in the stools of influenza patients.</p><h3>Objectives</h3><p dir="ltr">Here, we investigated the molecular and biological characteristics of intestinal influenza viruses and their potent...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , |
| منشور في: |
2021
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| الموضوعات: | |
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إضافة وسم
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| الملخص: | <p dir="ltr">Human influenza viruses are occasionally detected in the stools of influenza patients.</p><h3>Objectives</h3><p dir="ltr">Here, we investigated the molecular and biological characteristics of intestinal influenza viruses and their potential role in virus transmission.</p><h3>Methods</h3><p dir="ltr">Fecal samples were first screened for the presence of influenza viral RNA using RT-qPCR. Positive fecal samples were subjected to cell culture. Isolated viruses were then sequenced using MiSeq platform. Replication kinetics and receptor binding affinity were also evaluated.</p><h3>Results</h3><p dir="ltr">Influenza RNA was detected in stool samples of 41% (36/87) of influenza A positive patients. Among the 36 stool samples subjected to viral isolation, 5 showed virus growth. Sequence analysis of isolated viruses revealed two distinct mutation patterns in fecal viruses. Set I viruses was able to replicate to higher titers in cell culture despite the limited number of mutations (6 mutations) compared to set II viruses (>10 mutations). Functional analysis of both sets revealed the ability to replicate efficiently in differentiated human bronchial cells. Receptor binding testing has also demonstrated their ability to bind α 2,3 and α 2,6 sialic acid receptors.</p><h3>Conclusion</h3><p dir="ltr">The ability of fecal influenza viruses to replicate in intestinal cells and human 3D bronchial cells might suggest their possible contribution in virus transmission.</p><h2>Other Information</h2><p dir="ltr">Published in: Infection, Genetics and Evolution<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.meegid.2021.104972" target="_blank">https://dx.doi.org/10.1016/j.meegid.2021.104972</a></p> |
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