Molecular and biological characterization of influenza A viruses isolated from human fecal samples
<p dir="ltr">Human influenza viruses are occasionally detected in the stools of influenza patients.</p><h3>Objectives</h3><p dir="ltr">Here, we investigated the molecular and biological characteristics of intestinal influenza viruses and their potent...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , |
| منشور في: |
2021
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| الموضوعات: | |
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إضافة وسم
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| _version_ | 1864513546431234048 |
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| author | Hebah A. Al Khatib (10494573) |
| author2 | Peter V. Coyle (6837260) Muna A. Al Maslamani (14777263) Asmaa A. Al Thani (10494576) Sameer A. Pathan (9983452) Hadi M. Yassine (4675846) |
| author2_role | author author author author author |
| author_facet | Hebah A. Al Khatib (10494573) Peter V. Coyle (6837260) Muna A. Al Maslamani (14777263) Asmaa A. Al Thani (10494576) Sameer A. Pathan (9983452) Hadi M. Yassine (4675846) |
| author_role | author |
| dc.creator.none.fl_str_mv | Hebah A. Al Khatib (10494573) Peter V. Coyle (6837260) Muna A. Al Maslamani (14777263) Asmaa A. Al Thani (10494576) Sameer A. Pathan (9983452) Hadi M. Yassine (4675846) |
| dc.date.none.fl_str_mv | 2021-09-01T00:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1016/j.meegid.2021.104972 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Molecular_and_biological_characterization_of_influenza_A_viruses_isolated_from_human_fecal_samples/24433213 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Genetics Biomedical and clinical sciences Clinical sciences Influenza virus Intestinal replication Sialic acid receptors Transmission Virus diversity |
| dc.title.none.fl_str_mv | Molecular and biological characterization of influenza A viruses isolated from human fecal samples |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Human influenza viruses are occasionally detected in the stools of influenza patients.</p><h3>Objectives</h3><p dir="ltr">Here, we investigated the molecular and biological characteristics of intestinal influenza viruses and their potential role in virus transmission.</p><h3>Methods</h3><p dir="ltr">Fecal samples were first screened for the presence of influenza viral RNA using RT-qPCR. Positive fecal samples were subjected to cell culture. Isolated viruses were then sequenced using MiSeq platform. Replication kinetics and receptor binding affinity were also evaluated.</p><h3>Results</h3><p dir="ltr">Influenza RNA was detected in stool samples of 41% (36/87) of influenza A positive patients. Among the 36 stool samples subjected to viral isolation, 5 showed virus growth. Sequence analysis of isolated viruses revealed two distinct mutation patterns in fecal viruses. Set I viruses was able to replicate to higher titers in cell culture despite the limited number of mutations (6 mutations) compared to set II viruses (>10 mutations). Functional analysis of both sets revealed the ability to replicate efficiently in differentiated human bronchial cells. Receptor binding testing has also demonstrated their ability to bind α 2,3 and α 2,6 sialic acid receptors.</p><h3>Conclusion</h3><p dir="ltr">The ability of fecal influenza viruses to replicate in intestinal cells and human 3D bronchial cells might suggest their possible contribution in virus transmission.</p><h2>Other Information</h2><p dir="ltr">Published in: Infection, Genetics and Evolution<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.meegid.2021.104972" target="_blank">https://dx.doi.org/10.1016/j.meegid.2021.104972</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_4cecabb295f74dc8d00760ac92b42f60 |
| identifier_str_mv | 10.1016/j.meegid.2021.104972 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/24433213 |
| publishDate | 2021 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Molecular and biological characterization of influenza A viruses isolated from human fecal samplesHebah A. Al Khatib (10494573)Peter V. Coyle (6837260)Muna A. Al Maslamani (14777263)Asmaa A. Al Thani (10494576)Sameer A. Pathan (9983452)Hadi M. Yassine (4675846)Biological sciencesGeneticsBiomedical and clinical sciencesClinical sciencesInfluenza virusIntestinal replicationSialic acid receptorsTransmissionVirus diversity<p dir="ltr">Human influenza viruses are occasionally detected in the stools of influenza patients.</p><h3>Objectives</h3><p dir="ltr">Here, we investigated the molecular and biological characteristics of intestinal influenza viruses and their potential role in virus transmission.</p><h3>Methods</h3><p dir="ltr">Fecal samples were first screened for the presence of influenza viral RNA using RT-qPCR. Positive fecal samples were subjected to cell culture. Isolated viruses were then sequenced using MiSeq platform. Replication kinetics and receptor binding affinity were also evaluated.</p><h3>Results</h3><p dir="ltr">Influenza RNA was detected in stool samples of 41% (36/87) of influenza A positive patients. Among the 36 stool samples subjected to viral isolation, 5 showed virus growth. Sequence analysis of isolated viruses revealed two distinct mutation patterns in fecal viruses. Set I viruses was able to replicate to higher titers in cell culture despite the limited number of mutations (6 mutations) compared to set II viruses (>10 mutations). Functional analysis of both sets revealed the ability to replicate efficiently in differentiated human bronchial cells. Receptor binding testing has also demonstrated their ability to bind α 2,3 and α 2,6 sialic acid receptors.</p><h3>Conclusion</h3><p dir="ltr">The ability of fecal influenza viruses to replicate in intestinal cells and human 3D bronchial cells might suggest their possible contribution in virus transmission.</p><h2>Other Information</h2><p dir="ltr">Published in: Infection, Genetics and Evolution<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.meegid.2021.104972" target="_blank">https://dx.doi.org/10.1016/j.meegid.2021.104972</a></p>2021-09-01T00:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1016/j.meegid.2021.104972https://figshare.com/articles/journal_contribution/Molecular_and_biological_characterization_of_influenza_A_viruses_isolated_from_human_fecal_samples/24433213CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/244332132021-09-01T00:00:00Z |
| spellingShingle | Molecular and biological characterization of influenza A viruses isolated from human fecal samples Hebah A. Al Khatib (10494573) Biological sciences Genetics Biomedical and clinical sciences Clinical sciences Influenza virus Intestinal replication Sialic acid receptors Transmission Virus diversity |
| status_str | publishedVersion |
| title | Molecular and biological characterization of influenza A viruses isolated from human fecal samples |
| title_full | Molecular and biological characterization of influenza A viruses isolated from human fecal samples |
| title_fullStr | Molecular and biological characterization of influenza A viruses isolated from human fecal samples |
| title_full_unstemmed | Molecular and biological characterization of influenza A viruses isolated from human fecal samples |
| title_short | Molecular and biological characterization of influenza A viruses isolated from human fecal samples |
| title_sort | Molecular and biological characterization of influenza A viruses isolated from human fecal samples |
| topic | Biological sciences Genetics Biomedical and clinical sciences Clinical sciences Influenza virus Intestinal replication Sialic acid receptors Transmission Virus diversity |