Mft1, identified from a genome-wide screen of the yeast haploid mutants, mediates cell cycle arrest to counteract quinoxaline-induced toxicity
<p dir="ltr">Quinoxaline is a heterocyclic compound with a two-membered ring structure that undergoes redox cycling to produce toxic free radicals. It has antiviral, antibacterial, antifungal, and antitumor activities. However, the biological functions that are involved in mounting a...
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2024
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| author | Abdallah Alhaj Sulaiman (17777421) |
| author2 | Dana E. Al-Ansari (16327350) Reem Ali (9913494) Mustapha Aouida (417652) Dindial Ramotar (208416) |
| author2_role | author author author author |
| author_facet | Abdallah Alhaj Sulaiman (17777421) Dana E. Al-Ansari (16327350) Reem Ali (9913494) Mustapha Aouida (417652) Dindial Ramotar (208416) |
| author_role | author |
| dc.creator.none.fl_str_mv | Abdallah Alhaj Sulaiman (17777421) Dana E. Al-Ansari (16327350) Reem Ali (9913494) Mustapha Aouida (417652) Dindial Ramotar (208416) |
| dc.date.none.fl_str_mv | 2024-01-12T09:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3389/fgene.2023.1296383 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Mft1_identified_from_a_genome-wide_screen_of_the_yeast_haploid_mutants_mediates_cell_cycle_arrest_to_counteract_quinoxaline-induced_toxicity/26389078 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Biochemistry and cell biology Genetics Biomedical and clinical sciences Clinical sciences Saccharomyces cerevisiae quinoxaline sensitive mutants cell cycle arrest genome-wide screening drug resistance antibacterial antifungal antitumor activities |
| dc.title.none.fl_str_mv | Mft1, identified from a genome-wide screen of the yeast haploid mutants, mediates cell cycle arrest to counteract quinoxaline-induced toxicity |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Quinoxaline is a heterocyclic compound with a two-membered ring structure that undergoes redox cycling to produce toxic free radicals. It has antiviral, antibacterial, antifungal, and antitumor activities. However, the biological functions that are involved in mounting a response against the toxic effects of quinoxaline have not been investigated. Herein, we performed a genome-wide screen using the yeast haploid mutant collection and reported the identification of 12 mutants that displayed varying sensitivity towards quinoxaline. No mutant was recovered that showed resistance to quinoxaline. The quinoxaline-sensitive mutants were deleted for genes that encode cell cycle function, as well as genes that belong to other physiological pathways such as the vacuolar detoxification process. Three of the highly sensitive gene-deletion mutants lack the DDC1,<i> DUN1</i>, and <i>MFT1</i> genes. While Ddc1 and Dun1 are known to perform roles in the cell cycle arrest pathway, the role of Mft1 remains unclear. We show that the <i>mft1Δ</i> mutant is as sensitive to quinoxaline as the <i>ddc1Δ </i>mutant. However, the double mutant <i>ddc1Δ mft1Δ</i> lacking the <i>DDC1</i> and <i>MFT1</i> genes, is extremely sensitive to quinoxaline, as compared to the<i> ddc1Δ</i> and <i>mft1Δ</i> single mutants. We further show that the mft1Δ mutant is unable to arrest in the G2/M phase in response to the drug. We conclude that Mft1 performs a unique function independent of Ddc1 in the cell cycle arrest pathway in response to quinoxaline exposure. This is the first demonstration that quinoxaline exerts its toxic effect likely by inducing oxidative DNA damage causing cell cycle arrest. We suggest that clinical applications of quinoxaline and its derivatives should entail targeting cancer cells with defective cell cycle arrest.</p><h2>Other Information</h2><p dir="ltr">Published in: Frontiers in Genetics<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3389/fgene.2023.1296383" target="_blank">https://dx.doi.org/10.3389/fgene.2023.1296383</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_4e016be53c158183ac0ad965aff8b58e |
| identifier_str_mv | 10.3389/fgene.2023.1296383 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/26389078 |
| publishDate | 2024 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Mft1, identified from a genome-wide screen of the yeast haploid mutants, mediates cell cycle arrest to counteract quinoxaline-induced toxicityAbdallah Alhaj Sulaiman (17777421)Dana E. Al-Ansari (16327350)Reem Ali (9913494)Mustapha Aouida (417652)Dindial Ramotar (208416)Biological sciencesBiochemistry and cell biologyGeneticsBiomedical and clinical sciencesClinical sciencesSaccharomyces cerevisiaequinoxaline sensitive mutantscell cycle arrestgenome-wide screeningdrug resistanceantibacterialantifungalantitumor activities<p dir="ltr">Quinoxaline is a heterocyclic compound with a two-membered ring structure that undergoes redox cycling to produce toxic free radicals. It has antiviral, antibacterial, antifungal, and antitumor activities. However, the biological functions that are involved in mounting a response against the toxic effects of quinoxaline have not been investigated. Herein, we performed a genome-wide screen using the yeast haploid mutant collection and reported the identification of 12 mutants that displayed varying sensitivity towards quinoxaline. No mutant was recovered that showed resistance to quinoxaline. The quinoxaline-sensitive mutants were deleted for genes that encode cell cycle function, as well as genes that belong to other physiological pathways such as the vacuolar detoxification process. Three of the highly sensitive gene-deletion mutants lack the DDC1,<i> DUN1</i>, and <i>MFT1</i> genes. While Ddc1 and Dun1 are known to perform roles in the cell cycle arrest pathway, the role of Mft1 remains unclear. We show that the <i>mft1Δ</i> mutant is as sensitive to quinoxaline as the <i>ddc1Δ </i>mutant. However, the double mutant <i>ddc1Δ mft1Δ</i> lacking the <i>DDC1</i> and <i>MFT1</i> genes, is extremely sensitive to quinoxaline, as compared to the<i> ddc1Δ</i> and <i>mft1Δ</i> single mutants. We further show that the mft1Δ mutant is unable to arrest in the G2/M phase in response to the drug. We conclude that Mft1 performs a unique function independent of Ddc1 in the cell cycle arrest pathway in response to quinoxaline exposure. This is the first demonstration that quinoxaline exerts its toxic effect likely by inducing oxidative DNA damage causing cell cycle arrest. We suggest that clinical applications of quinoxaline and its derivatives should entail targeting cancer cells with defective cell cycle arrest.</p><h2>Other Information</h2><p dir="ltr">Published in: Frontiers in Genetics<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3389/fgene.2023.1296383" target="_blank">https://dx.doi.org/10.3389/fgene.2023.1296383</a></p>2024-01-12T09:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3389/fgene.2023.1296383https://figshare.com/articles/journal_contribution/Mft1_identified_from_a_genome-wide_screen_of_the_yeast_haploid_mutants_mediates_cell_cycle_arrest_to_counteract_quinoxaline-induced_toxicity/26389078CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/263890782024-01-12T09:00:00Z |
| spellingShingle | Mft1, identified from a genome-wide screen of the yeast haploid mutants, mediates cell cycle arrest to counteract quinoxaline-induced toxicity Abdallah Alhaj Sulaiman (17777421) Biological sciences Biochemistry and cell biology Genetics Biomedical and clinical sciences Clinical sciences Saccharomyces cerevisiae quinoxaline sensitive mutants cell cycle arrest genome-wide screening drug resistance antibacterial antifungal antitumor activities |
| status_str | publishedVersion |
| title | Mft1, identified from a genome-wide screen of the yeast haploid mutants, mediates cell cycle arrest to counteract quinoxaline-induced toxicity |
| title_full | Mft1, identified from a genome-wide screen of the yeast haploid mutants, mediates cell cycle arrest to counteract quinoxaline-induced toxicity |
| title_fullStr | Mft1, identified from a genome-wide screen of the yeast haploid mutants, mediates cell cycle arrest to counteract quinoxaline-induced toxicity |
| title_full_unstemmed | Mft1, identified from a genome-wide screen of the yeast haploid mutants, mediates cell cycle arrest to counteract quinoxaline-induced toxicity |
| title_short | Mft1, identified from a genome-wide screen of the yeast haploid mutants, mediates cell cycle arrest to counteract quinoxaline-induced toxicity |
| title_sort | Mft1, identified from a genome-wide screen of the yeast haploid mutants, mediates cell cycle arrest to counteract quinoxaline-induced toxicity |
| topic | Biological sciences Biochemistry and cell biology Genetics Biomedical and clinical sciences Clinical sciences Saccharomyces cerevisiae quinoxaline sensitive mutants cell cycle arrest genome-wide screening drug resistance antibacterial antifungal antitumor activities |