Predictive value of tyrosine phosphatase receptor gamma for the response to treatment tyrosine kinase inhibitors in chronic myeloid leukemia patients
<p>Protein tyrosine phosphatase receptor gamma (PTPRG) is a member of the receptor-like family protein tyrosine phosphatases and acts as a tumor suppressor gene in different neoplasms. Recent studies reported the down-regulation of PTPRG expression levels in Chronic Myeloid Leukemia disease (C...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , , , , , , , , |
| منشور في: |
2022
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| _version_ | 1864513566712791040 |
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| author | Mohamed A. Ismail (418629) |
| author2 | Marzia Vezzalini (212801) Hisham Morsi (14152881) Ahmad Abujaber (9100064) Ali Al Sayab (4166521) Kodappully Siveen (3520073) Mohamed A. Yassin (8361183) Maria Monne (4166524) Muthanna Samara (11619435) Richard Cook (70389) Claudio Sorio (212799) Helmout Modjtahedi (532099) Nader I. Al-Dewik (14152884) |
| author2_role | author author author author author author author author author author author author |
| author_facet | Mohamed A. Ismail (418629) Marzia Vezzalini (212801) Hisham Morsi (14152881) Ahmad Abujaber (9100064) Ali Al Sayab (4166521) Kodappully Siveen (3520073) Mohamed A. Yassin (8361183) Maria Monne (4166524) Muthanna Samara (11619435) Richard Cook (70389) Claudio Sorio (212799) Helmout Modjtahedi (532099) Nader I. Al-Dewik (14152884) |
| author_role | author |
| dc.creator.none.fl_str_mv | Mohamed A. Ismail (418629) Marzia Vezzalini (212801) Hisham Morsi (14152881) Ahmad Abujaber (9100064) Ali Al Sayab (4166521) Kodappully Siveen (3520073) Mohamed A. Yassin (8361183) Maria Monne (4166524) Muthanna Samara (11619435) Richard Cook (70389) Claudio Sorio (212799) Helmout Modjtahedi (532099) Nader I. Al-Dewik (14152884) |
| dc.date.none.fl_str_mv | 2022-11-22T21:17:14Z |
| dc.identifier.none.fl_str_mv | 10.1038/s41598-021-86875-y |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Predictive_value_of_tyrosine_phosphatase_receptor_gamma_for_the_response_to_treatment_tyrosine_kinase_inhibitors_in_chronic_myeloid_leukemia_patients/21598212 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Oncology and carcinogenesis Multidisciplinary |
| dc.title.none.fl_str_mv | Predictive value of tyrosine phosphatase receptor gamma for the response to treatment tyrosine kinase inhibitors in chronic myeloid leukemia patients |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p>Protein tyrosine phosphatase receptor gamma (PTPRG) is a member of the receptor-like family protein tyrosine phosphatases and acts as a tumor suppressor gene in different neoplasms. Recent studies reported the down-regulation of PTPRG expression levels in Chronic Myeloid Leukemia disease (CML). In addition, the BCR-ABL1 transcript level is currently a key predictive biomarker of CML response to treatment with Tyrosine Kinase Inhibitors (TKIs). The aim of this study was to employ flow cytometry to monitor the changes in the expression level of PTPRG in the white blood cells (WBCs) of CML patients at the time of diagnosis and following treatment with TKIs. WBCs from peripheral blood of 21 CML patients were extracted at diagnosis and during follow up along with seven healthy individuals. The PTPRG expression level was determined at protein and mRNA levels by both flow cytometry with monoclonal antibody (TPγ B9-2) and RT-qPCR, and BCR-ABL1 transcript by RT-qPCR, respectively. PTPRG expression was found to be lower in the neutrophils and monocytes of CML patients at time of diagnosis compared to healthy individuals. Treatment with TKIs nilotinib and Imatinib Mesylate restored the expression of PTPRG in the WBCs of CML patients to levels observed in healthy controls. Moreover, restoration levels were greatest in optimal responders and occurred earlier with nilotinib compared to imatinib. Our results support the measurement of PTPRG expression level in the WBCs of CML patients by flow cytometry as a monitoring tool for the response to treatment with TKIs in CML patients.</p><h2>Other Information</h2> <p> Published in: Scientific Reports<br> License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="http://dx.doi.org/10.1038/s41598-021-86875-y" target="_blank">http://dx.doi.org/10.1038/s41598-021-86875-y</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_4e4934f0b2c318888615a7799e8703f2 |
| identifier_str_mv | 10.1038/s41598-021-86875-y |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/21598212 |
| publishDate | 2022 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Predictive value of tyrosine phosphatase receptor gamma for the response to treatment tyrosine kinase inhibitors in chronic myeloid leukemia patientsMohamed A. Ismail (418629)Marzia Vezzalini (212801)Hisham Morsi (14152881)Ahmad Abujaber (9100064)Ali Al Sayab (4166521)Kodappully Siveen (3520073)Mohamed A. Yassin (8361183)Maria Monne (4166524)Muthanna Samara (11619435)Richard Cook (70389)Claudio Sorio (212799)Helmout Modjtahedi (532099)Nader I. Al-Dewik (14152884)Oncology and carcinogenesisMultidisciplinary<p>Protein tyrosine phosphatase receptor gamma (PTPRG) is a member of the receptor-like family protein tyrosine phosphatases and acts as a tumor suppressor gene in different neoplasms. Recent studies reported the down-regulation of PTPRG expression levels in Chronic Myeloid Leukemia disease (CML). In addition, the BCR-ABL1 transcript level is currently a key predictive biomarker of CML response to treatment with Tyrosine Kinase Inhibitors (TKIs). The aim of this study was to employ flow cytometry to monitor the changes in the expression level of PTPRG in the white blood cells (WBCs) of CML patients at the time of diagnosis and following treatment with TKIs. WBCs from peripheral blood of 21 CML patients were extracted at diagnosis and during follow up along with seven healthy individuals. The PTPRG expression level was determined at protein and mRNA levels by both flow cytometry with monoclonal antibody (TPγ B9-2) and RT-qPCR, and BCR-ABL1 transcript by RT-qPCR, respectively. PTPRG expression was found to be lower in the neutrophils and monocytes of CML patients at time of diagnosis compared to healthy individuals. Treatment with TKIs nilotinib and Imatinib Mesylate restored the expression of PTPRG in the WBCs of CML patients to levels observed in healthy controls. Moreover, restoration levels were greatest in optimal responders and occurred earlier with nilotinib compared to imatinib. Our results support the measurement of PTPRG expression level in the WBCs of CML patients by flow cytometry as a monitoring tool for the response to treatment with TKIs in CML patients.</p><h2>Other Information</h2> <p> Published in: Scientific Reports<br> License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="http://dx.doi.org/10.1038/s41598-021-86875-y" target="_blank">http://dx.doi.org/10.1038/s41598-021-86875-y</a></p>2022-11-22T21:17:14ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1038/s41598-021-86875-yhttps://figshare.com/articles/journal_contribution/Predictive_value_of_tyrosine_phosphatase_receptor_gamma_for_the_response_to_treatment_tyrosine_kinase_inhibitors_in_chronic_myeloid_leukemia_patients/21598212CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/215982122022-11-22T21:17:14Z |
| spellingShingle | Predictive value of tyrosine phosphatase receptor gamma for the response to treatment tyrosine kinase inhibitors in chronic myeloid leukemia patients Mohamed A. Ismail (418629) Oncology and carcinogenesis Multidisciplinary |
| status_str | publishedVersion |
| title | Predictive value of tyrosine phosphatase receptor gamma for the response to treatment tyrosine kinase inhibitors in chronic myeloid leukemia patients |
| title_full | Predictive value of tyrosine phosphatase receptor gamma for the response to treatment tyrosine kinase inhibitors in chronic myeloid leukemia patients |
| title_fullStr | Predictive value of tyrosine phosphatase receptor gamma for the response to treatment tyrosine kinase inhibitors in chronic myeloid leukemia patients |
| title_full_unstemmed | Predictive value of tyrosine phosphatase receptor gamma for the response to treatment tyrosine kinase inhibitors in chronic myeloid leukemia patients |
| title_short | Predictive value of tyrosine phosphatase receptor gamma for the response to treatment tyrosine kinase inhibitors in chronic myeloid leukemia patients |
| title_sort | Predictive value of tyrosine phosphatase receptor gamma for the response to treatment tyrosine kinase inhibitors in chronic myeloid leukemia patients |
| topic | Oncology and carcinogenesis Multidisciplinary |