Increased MicroRNA Levels in Women With Polycystic Ovarian Syndrome but Without Insulin Resistance: A Pilot Prospective Study
<h3>Background</h3><p dir="ltr">Small noncoding microRNA (miRNA) have regulatory functions in polycystic ovary syndrome (PCOS) that differ to those in women without PCOS. However, little is known about miRNA expression in women with PCOS who are not insulin resistant (IR)...
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| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , , , , , , |
| منشور في: |
2020
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| الموضوعات: | |
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| _version_ | 1864513557697134592 |
|---|---|
| author | Alexandra E. Butler (6189536) |
| author2 | Vimal Ramachandran (8774123) Thomas Keith Cunningham (9442886) Rhiannon David (8774129) Nigel J. Gooderham (704792) Manasi Benurwar (8774132) Soha R. Dargham (3613250) Shahina Hayat (8774135) Thozhukat Sathyapalan (704787) S Hani Najafi-Shoushtari (9442889) Stephen L. Atkin (6684368) |
| author2_role | author author author author author author author author author author |
| author_facet | Alexandra E. Butler (6189536) Vimal Ramachandran (8774123) Thomas Keith Cunningham (9442886) Rhiannon David (8774129) Nigel J. Gooderham (704792) Manasi Benurwar (8774132) Soha R. Dargham (3613250) Shahina Hayat (8774135) Thozhukat Sathyapalan (704787) S Hani Najafi-Shoushtari (9442889) Stephen L. Atkin (6684368) |
| author_role | author |
| dc.creator.none.fl_str_mv | Alexandra E. Butler (6189536) Vimal Ramachandran (8774123) Thomas Keith Cunningham (9442886) Rhiannon David (8774129) Nigel J. Gooderham (704792) Manasi Benurwar (8774132) Soha R. Dargham (3613250) Shahina Hayat (8774135) Thozhukat Sathyapalan (704787) S Hani Najafi-Shoushtari (9442889) Stephen L. Atkin (6684368) |
| dc.date.none.fl_str_mv | 2020-09-30T09:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3389/fendo.2020.571357 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Increased_MicroRNA_Levels_in_Women_With_Polycystic_Ovarian_Syndrome_but_Without_Insulin_Resistance_A_Pilot_Prospective_Study/27044434 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Genetics Biomedical and clinical sciences Clinical sciences Medical biochemistry and metabolomics microRNA polycystic ovary syndrome non-obese, insulin sensitivity anti-Mullerian hormone female |
| dc.title.none.fl_str_mv | Increased MicroRNA Levels in Women With Polycystic Ovarian Syndrome but Without Insulin Resistance: A Pilot Prospective Study |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <h3>Background</h3><p dir="ltr">Small noncoding microRNA (miRNA) have regulatory functions in polycystic ovary syndrome (PCOS) that differ to those in women without PCOS. However, little is known about miRNA expression in women with PCOS who are not insulin resistant (IR).</p><h3>Methods</h3><p dir="ltr">Circulating miRNAs were measured using quantitative polymerase chain reaction (qPCR) in 24 non-obese BMI and age matched women with PCOS and 24 control women. A miRNA data set was used to determine miRNA levels.</p><h3>Results</h3><p dir="ltr">Women with PCOS showed a higher free androgen index (FAI) and anti-mullerian hormone (AMH) but IR did not differ. Four miRNAs (miR-1260a, miR-18b-5p, miR-424-5p, and miR let-7b-3p) differed between control and PCOS women that passed the false discovery rate (FDR) out of a total of 177 circulating miRNAs that were detected. MiRNA let-7b-3p correlated with AMH in PCOS (p < 0.05). When the groups were combined, miR-1260a correlated with FAI and let-7b-3p correlated with body mass index (BMI) (p < 0.05). There was no correlation to androgen levels. Ingenuity pathway analysis showed that nine of the top 10 miRNAs reported were associated with inflammatory pathways.</p><h3>Conclusion</h3><p dir="ltr">When IR did not differ between PCOS and control women, only four miRNA differed significantly suggesting that IR may be a driver for many of the miRNA changes reported. Let-7b-3p was related to AMH in PCOS, and to BMI as a group, whilst miR-1260a correlated with FAI. Androgen levels, however, had no effect upon circulating miRNA profiles. The expressed miRNAs were associated with the inflammatory pathway involving TNF and IL6.</p><h2>Other Information</h2><p dir="ltr">Published in: Frontiers in Endocrinology<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3389/fendo.2020.571357" target="_blank">https://dx.doi.org/10.3389/fendo.2020.571357</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_4eb074d4c35aeb54ee39aa22f75e07fb |
| identifier_str_mv | 10.3389/fendo.2020.571357 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/27044434 |
| publishDate | 2020 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Increased MicroRNA Levels in Women With Polycystic Ovarian Syndrome but Without Insulin Resistance: A Pilot Prospective StudyAlexandra E. Butler (6189536)Vimal Ramachandran (8774123)Thomas Keith Cunningham (9442886)Rhiannon David (8774129)Nigel J. Gooderham (704792)Manasi Benurwar (8774132)Soha R. Dargham (3613250)Shahina Hayat (8774135)Thozhukat Sathyapalan (704787)S Hani Najafi-Shoushtari (9442889)Stephen L. Atkin (6684368)Biological sciencesGeneticsBiomedical and clinical sciencesClinical sciencesMedical biochemistry and metabolomicsmicroRNApolycystic ovary syndromenon-obese, insulin sensitivityanti-Mullerian hormonefemale<h3>Background</h3><p dir="ltr">Small noncoding microRNA (miRNA) have regulatory functions in polycystic ovary syndrome (PCOS) that differ to those in women without PCOS. However, little is known about miRNA expression in women with PCOS who are not insulin resistant (IR).</p><h3>Methods</h3><p dir="ltr">Circulating miRNAs were measured using quantitative polymerase chain reaction (qPCR) in 24 non-obese BMI and age matched women with PCOS and 24 control women. A miRNA data set was used to determine miRNA levels.</p><h3>Results</h3><p dir="ltr">Women with PCOS showed a higher free androgen index (FAI) and anti-mullerian hormone (AMH) but IR did not differ. Four miRNAs (miR-1260a, miR-18b-5p, miR-424-5p, and miR let-7b-3p) differed between control and PCOS women that passed the false discovery rate (FDR) out of a total of 177 circulating miRNAs that were detected. MiRNA let-7b-3p correlated with AMH in PCOS (p < 0.05). When the groups were combined, miR-1260a correlated with FAI and let-7b-3p correlated with body mass index (BMI) (p < 0.05). There was no correlation to androgen levels. Ingenuity pathway analysis showed that nine of the top 10 miRNAs reported were associated with inflammatory pathways.</p><h3>Conclusion</h3><p dir="ltr">When IR did not differ between PCOS and control women, only four miRNA differed significantly suggesting that IR may be a driver for many of the miRNA changes reported. Let-7b-3p was related to AMH in PCOS, and to BMI as a group, whilst miR-1260a correlated with FAI. Androgen levels, however, had no effect upon circulating miRNA profiles. The expressed miRNAs were associated with the inflammatory pathway involving TNF and IL6.</p><h2>Other Information</h2><p dir="ltr">Published in: Frontiers in Endocrinology<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3389/fendo.2020.571357" target="_blank">https://dx.doi.org/10.3389/fendo.2020.571357</a></p>2020-09-30T09:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3389/fendo.2020.571357https://figshare.com/articles/journal_contribution/Increased_MicroRNA_Levels_in_Women_With_Polycystic_Ovarian_Syndrome_but_Without_Insulin_Resistance_A_Pilot_Prospective_Study/27044434CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/270444342020-09-30T09:00:00Z |
| spellingShingle | Increased MicroRNA Levels in Women With Polycystic Ovarian Syndrome but Without Insulin Resistance: A Pilot Prospective Study Alexandra E. Butler (6189536) Biological sciences Genetics Biomedical and clinical sciences Clinical sciences Medical biochemistry and metabolomics microRNA polycystic ovary syndrome non-obese, insulin sensitivity anti-Mullerian hormone female |
| status_str | publishedVersion |
| title | Increased MicroRNA Levels in Women With Polycystic Ovarian Syndrome but Without Insulin Resistance: A Pilot Prospective Study |
| title_full | Increased MicroRNA Levels in Women With Polycystic Ovarian Syndrome but Without Insulin Resistance: A Pilot Prospective Study |
| title_fullStr | Increased MicroRNA Levels in Women With Polycystic Ovarian Syndrome but Without Insulin Resistance: A Pilot Prospective Study |
| title_full_unstemmed | Increased MicroRNA Levels in Women With Polycystic Ovarian Syndrome but Without Insulin Resistance: A Pilot Prospective Study |
| title_short | Increased MicroRNA Levels in Women With Polycystic Ovarian Syndrome but Without Insulin Resistance: A Pilot Prospective Study |
| title_sort | Increased MicroRNA Levels in Women With Polycystic Ovarian Syndrome but Without Insulin Resistance: A Pilot Prospective Study |
| topic | Biological sciences Genetics Biomedical and clinical sciences Clinical sciences Medical biochemistry and metabolomics microRNA polycystic ovary syndrome non-obese, insulin sensitivity anti-Mullerian hormone female |