Novel Prosopis juliflora leaf ethanolic extract as natural antimicrobial agent against food spoiling microorganisms

<p>Fresh produces spoilage is a worldwide concern that accompany the global increase in food demand. Adverse human health and environmental effects of commercial spoilage control agents are major public concern. In this study, Prosopis juliflora leaves and fruit extracts had their antimicrobia...

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Main Author: Iman Saleh (14152878) (author)
Other Authors: Mohammed H. Abu-Dieyeh (14152761) (author)
Published: 2022
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author Iman Saleh (14152878)
author2 Mohammed H. Abu-Dieyeh (14152761)
author2_role author
author_facet Iman Saleh (14152878)
Mohammed H. Abu-Dieyeh (14152761)
author_role author
dc.creator.none.fl_str_mv Iman Saleh (14152878)
Mohammed H. Abu-Dieyeh (14152761)
dc.date.none.fl_str_mv 2022-11-22T21:17:13Z
dc.identifier.none.fl_str_mv 10.1038/s41598-021-86509-3
dc.relation.none.fl_str_mv https://figshare.com/articles/journal_contribution/Novel_Prosopis_juliflora_leaf_ethanolic_extract_as_natural_antimicrobial_agent_against_food_spoiling_microorganisms/21598203
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Microbiology
Multidisciplinary
dc.title.none.fl_str_mv Novel Prosopis juliflora leaf ethanolic extract as natural antimicrobial agent against food spoiling microorganisms
dc.type.none.fl_str_mv Text
Journal contribution
info:eu-repo/semantics/publishedVersion
text
contribution to journal
description <p>Fresh produces spoilage is a worldwide concern that accompany the global increase in food demand. Adverse human health and environmental effects of commercial spoilage control agents are major public concern. In this study, Prosopis juliflora leaves and fruit extracts had their antimicrobial activities evaluated against the growth of selected bacteria and yeast, and against mycelial growth and conidial germination of selected mycotoxins-producing fungi. P. juliflora water-soluble leaf ethanolic (PJ-WS-LE) extract with its novel extraction method showed the strongest antibacterial activity. Antimicrobial tests showed total inhibition of Botrytis cinerea, Alternaria alternata, Bacillus subtilis, Staphylococcus aureus and Candida albicans with MICs ranging between 0.125 and 1 mg/ml. Percent inhibition of mycelial growth (PIMG) of the extract was also determined against seven other fungal strains with highest value against Geotrichum candidum (66.2%). Even the least affected fungal strain showed alterations in their hyphae and spores exposed to PJ-WS-LE extract when observed using scanning electron microscope (SEM), alterations include exfoliated flakes, pores, vacuolation and applanation. Small-scale fruit bioassays controlled experiment showed high efficacy of the extract in protecting inoculated cherry tomato samples from B. cinerea and A. alternata infections. In conclusion, PJ-WS-LE extract is a feasible, natural antifungal agent that can replace common anti-spoiling chemicals.</p><h2>Other Information</h2> <p> Published in: Scientific Reports<br> License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="http://dx.doi.org/10.1038/s41598-021-86509-3" target="_blank">http://dx.doi.org/10.1038/s41598-021-86509-3</a></p>
eu_rights_str_mv openAccess
id Manara2_51b6d24a15cd88f2ee665fb172b07487
identifier_str_mv 10.1038/s41598-021-86509-3
network_acronym_str Manara2
network_name_str Manara2
oai_identifier_str oai:figshare.com:article/21598203
publishDate 2022
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rights_invalid_str_mv CC BY 4.0
spelling Novel Prosopis juliflora leaf ethanolic extract as natural antimicrobial agent against food spoiling microorganismsIman Saleh (14152878)Mohammed H. Abu-Dieyeh (14152761)MicrobiologyMultidisciplinary<p>Fresh produces spoilage is a worldwide concern that accompany the global increase in food demand. Adverse human health and environmental effects of commercial spoilage control agents are major public concern. In this study, Prosopis juliflora leaves and fruit extracts had their antimicrobial activities evaluated against the growth of selected bacteria and yeast, and against mycelial growth and conidial germination of selected mycotoxins-producing fungi. P. juliflora water-soluble leaf ethanolic (PJ-WS-LE) extract with its novel extraction method showed the strongest antibacterial activity. Antimicrobial tests showed total inhibition of Botrytis cinerea, Alternaria alternata, Bacillus subtilis, Staphylococcus aureus and Candida albicans with MICs ranging between 0.125 and 1 mg/ml. Percent inhibition of mycelial growth (PIMG) of the extract was also determined against seven other fungal strains with highest value against Geotrichum candidum (66.2%). Even the least affected fungal strain showed alterations in their hyphae and spores exposed to PJ-WS-LE extract when observed using scanning electron microscope (SEM), alterations include exfoliated flakes, pores, vacuolation and applanation. Small-scale fruit bioassays controlled experiment showed high efficacy of the extract in protecting inoculated cherry tomato samples from B. cinerea and A. alternata infections. In conclusion, PJ-WS-LE extract is a feasible, natural antifungal agent that can replace common anti-spoiling chemicals.</p><h2>Other Information</h2> <p> Published in: Scientific Reports<br> License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="http://dx.doi.org/10.1038/s41598-021-86509-3" target="_blank">http://dx.doi.org/10.1038/s41598-021-86509-3</a></p>2022-11-22T21:17:13ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1038/s41598-021-86509-3https://figshare.com/articles/journal_contribution/Novel_Prosopis_juliflora_leaf_ethanolic_extract_as_natural_antimicrobial_agent_against_food_spoiling_microorganisms/21598203CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/215982032022-11-22T21:17:13Z
spellingShingle Novel Prosopis juliflora leaf ethanolic extract as natural antimicrobial agent against food spoiling microorganisms
Iman Saleh (14152878)
Microbiology
Multidisciplinary
status_str publishedVersion
title Novel Prosopis juliflora leaf ethanolic extract as natural antimicrobial agent against food spoiling microorganisms
title_full Novel Prosopis juliflora leaf ethanolic extract as natural antimicrobial agent against food spoiling microorganisms
title_fullStr Novel Prosopis juliflora leaf ethanolic extract as natural antimicrobial agent against food spoiling microorganisms
title_full_unstemmed Novel Prosopis juliflora leaf ethanolic extract as natural antimicrobial agent against food spoiling microorganisms
title_short Novel Prosopis juliflora leaf ethanolic extract as natural antimicrobial agent against food spoiling microorganisms
title_sort Novel Prosopis juliflora leaf ethanolic extract as natural antimicrobial agent against food spoiling microorganisms
topic Microbiology
Multidisciplinary