Comprehensive Transcriptome and Pathway Analyses Revealed Central Role for Fascin in Promoting Triple-Negative Breast Cancer Progression
<p dir="ltr">Recent years have witnessed major progress in development of novel therapeutic agents such as chemotherapy, targeted therapy and immune checkpoint inhibitors for breast cancer. However, cancer-related death remains high especially in triple-negative breast cancer (TNBC)...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , , , , |
| منشور في: |
2021
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| الموضوعات: | |
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| _version_ | 1864513517662502912 |
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| author | Rayanah Barnawi (8737584) |
| author2 | Samiyah Al-Khaldi (8737587) Salma Majid (18460644) Amal Qattan (4625467) Tala Bakheet (13921) Mohannad Fallatah (8737590) Hazem Ghebeh (79415) Nehad M. Alajez (7397276) Monther Al-Alwan (197831) |
| author2_role | author author author author author author author author |
| author_facet | Rayanah Barnawi (8737584) Samiyah Al-Khaldi (8737587) Salma Majid (18460644) Amal Qattan (4625467) Tala Bakheet (13921) Mohannad Fallatah (8737590) Hazem Ghebeh (79415) Nehad M. Alajez (7397276) Monther Al-Alwan (197831) |
| author_role | author |
| dc.creator.none.fl_str_mv | Rayanah Barnawi (8737584) Samiyah Al-Khaldi (8737587) Salma Majid (18460644) Amal Qattan (4625467) Tala Bakheet (13921) Mohannad Fallatah (8737590) Hazem Ghebeh (79415) Nehad M. Alajez (7397276) Monther Al-Alwan (197831) |
| dc.date.none.fl_str_mv | 2021-11-26T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3390/ph14121228 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Comprehensive_Transcriptome_and_Pathway_Analyses_Revealed_Central_Role_for_Fascin_in_Promoting_Triple-Negative_Breast_Cancer_Progression/25713822 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Oncology and carcinogenesis fascin breast cancer miRNA pathway analysis transcriptome IPA |
| dc.title.none.fl_str_mv | Comprehensive Transcriptome and Pathway Analyses Revealed Central Role for Fascin in Promoting Triple-Negative Breast Cancer Progression |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Recent years have witnessed major progress in development of novel therapeutic agents such as chemotherapy, targeted therapy and immune checkpoint inhibitors for breast cancer. However, cancer-related death remains high especially in triple-negative breast cancer (TNBC) due limited therapeutic options. Development of targeted therapies for TNBC requires better understanding of biology and signaling networks that promote disease progression. Fascin, an actin bundling protein, was identified as a key regulator of many signaling pathways that contribute to breast cancer progression. Herein, fascin ShRNA was used to generate stable fascin knockdown (FSCN1<sup>KD</sup>) in the MDA-MB-231 TNBC cell line and then were subjected to comprehensive mRNA and miRNA transcriptome analysis. We identified 129 upregulated and 114 downregulated mRNA transcripts, while 14 miRNAs were differentially expressed in FSCN1<sup>KD</sup>. Ingenuity pathway analysis (IPA) was used to predict the impact of differentially expressed transcripts on signaling pathways and functional categories and to construct miRNA-mRNA regulatory networks in the context of FSCN1 knockdown. Compared to FSCN1<sup>KD</sup>, fascin-positive (FSCN1<sup>CON</sup>) breast cancer cells showed enrichment in genes promoting cellular proliferation, migration, survival, DNA replication and repair. Expression of FSCN1<sup>high</sup> (identified in BRCA dataset from TCGA) in conjunction with elevated expression of the top 10 upregulated or decreased expression of the top 10 downregulated genes (identified in our FSCN1<sup>CON</sup> vs. FSCN1<sup>KD</sup>) correlates with worst survival outcome. Taken together, these data confirmed fascin’s role in promoting TNBC progression, and identified a novel opportunity for therapeutic interventions via targeting those FSCN1-related transcripts.</p><p dir="ltr"><br></p><h2>Other Information</h2><p dir="ltr">Published in: Pharmaceuticals<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/ph14121228" target="_blank">https://dx.doi.org/10.3390/ph14121228</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_51fb3c8d1d9f1bc76d5d7f90dc3c0155 |
| identifier_str_mv | 10.3390/ph14121228 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25713822 |
| publishDate | 2021 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Comprehensive Transcriptome and Pathway Analyses Revealed Central Role for Fascin in Promoting Triple-Negative Breast Cancer ProgressionRayanah Barnawi (8737584)Samiyah Al-Khaldi (8737587)Salma Majid (18460644)Amal Qattan (4625467)Tala Bakheet (13921)Mohannad Fallatah (8737590)Hazem Ghebeh (79415)Nehad M. Alajez (7397276)Monther Al-Alwan (197831)Biomedical and clinical sciencesOncology and carcinogenesisfascinbreast cancermiRNApathway analysistranscriptomeIPA<p dir="ltr">Recent years have witnessed major progress in development of novel therapeutic agents such as chemotherapy, targeted therapy and immune checkpoint inhibitors for breast cancer. However, cancer-related death remains high especially in triple-negative breast cancer (TNBC) due limited therapeutic options. Development of targeted therapies for TNBC requires better understanding of biology and signaling networks that promote disease progression. Fascin, an actin bundling protein, was identified as a key regulator of many signaling pathways that contribute to breast cancer progression. Herein, fascin ShRNA was used to generate stable fascin knockdown (FSCN1<sup>KD</sup>) in the MDA-MB-231 TNBC cell line and then were subjected to comprehensive mRNA and miRNA transcriptome analysis. We identified 129 upregulated and 114 downregulated mRNA transcripts, while 14 miRNAs were differentially expressed in FSCN1<sup>KD</sup>. Ingenuity pathway analysis (IPA) was used to predict the impact of differentially expressed transcripts on signaling pathways and functional categories and to construct miRNA-mRNA regulatory networks in the context of FSCN1 knockdown. Compared to FSCN1<sup>KD</sup>, fascin-positive (FSCN1<sup>CON</sup>) breast cancer cells showed enrichment in genes promoting cellular proliferation, migration, survival, DNA replication and repair. Expression of FSCN1<sup>high</sup> (identified in BRCA dataset from TCGA) in conjunction with elevated expression of the top 10 upregulated or decreased expression of the top 10 downregulated genes (identified in our FSCN1<sup>CON</sup> vs. FSCN1<sup>KD</sup>) correlates with worst survival outcome. Taken together, these data confirmed fascin’s role in promoting TNBC progression, and identified a novel opportunity for therapeutic interventions via targeting those FSCN1-related transcripts.</p><p dir="ltr"><br></p><h2>Other Information</h2><p dir="ltr">Published in: Pharmaceuticals<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/ph14121228" target="_blank">https://dx.doi.org/10.3390/ph14121228</a></p>2021-11-26T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3390/ph14121228https://figshare.com/articles/journal_contribution/Comprehensive_Transcriptome_and_Pathway_Analyses_Revealed_Central_Role_for_Fascin_in_Promoting_Triple-Negative_Breast_Cancer_Progression/25713822CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/257138222021-11-26T03:00:00Z |
| spellingShingle | Comprehensive Transcriptome and Pathway Analyses Revealed Central Role for Fascin in Promoting Triple-Negative Breast Cancer Progression Rayanah Barnawi (8737584) Biomedical and clinical sciences Oncology and carcinogenesis fascin breast cancer miRNA pathway analysis transcriptome IPA |
| status_str | publishedVersion |
| title | Comprehensive Transcriptome and Pathway Analyses Revealed Central Role for Fascin in Promoting Triple-Negative Breast Cancer Progression |
| title_full | Comprehensive Transcriptome and Pathway Analyses Revealed Central Role for Fascin in Promoting Triple-Negative Breast Cancer Progression |
| title_fullStr | Comprehensive Transcriptome and Pathway Analyses Revealed Central Role for Fascin in Promoting Triple-Negative Breast Cancer Progression |
| title_full_unstemmed | Comprehensive Transcriptome and Pathway Analyses Revealed Central Role for Fascin in Promoting Triple-Negative Breast Cancer Progression |
| title_short | Comprehensive Transcriptome and Pathway Analyses Revealed Central Role for Fascin in Promoting Triple-Negative Breast Cancer Progression |
| title_sort | Comprehensive Transcriptome and Pathway Analyses Revealed Central Role for Fascin in Promoting Triple-Negative Breast Cancer Progression |
| topic | Biomedical and clinical sciences Oncology and carcinogenesis fascin breast cancer miRNA pathway analysis transcriptome IPA |