Bacillus thuringiensis strain QBT220 pBtoxis plasmid structural instability enhances δ-endotoxins synthesis and bioinsecticidal activity
<p dir="ltr"><i>Bacillus thuringiensis</i> subsp. <i>israelensis</i> (<i>Bti</i>) spherical parasporal crystal contains several insecticidal proteins used as environmentally safe alternative to toxic chemical pesticides. The exploration of a <i&...
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| مؤلفون آخرون: | , |
| منشور في: |
2021
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| _version_ | 1864513552568549376 |
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| author | Kavita Nair (17151127) |
| author2 | Roda Al-Thani (540907) Samir Jaoua (11624805) |
| author2_role | author author |
| author_facet | Kavita Nair (17151127) Roda Al-Thani (540907) Samir Jaoua (11624805) |
| author_role | author |
| dc.creator.none.fl_str_mv | Kavita Nair (17151127) Roda Al-Thani (540907) Samir Jaoua (11624805) |
| dc.date.none.fl_str_mv | 2021-12-25T00:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1016/j.ecoenv.2021.112975 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Bacillus_thuringiensis_strain_QBT220_pBtoxis_plasmid_structural_instability_enhances_-endotoxins_synthesis_and_bioinsecticidal_activity/24314338 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Medical microbiology Pharmacology and pharmaceutical sciences Environmental sciences Environmental management Bacillus thuringiensis PBtoxis plasmid instability Bioinsecticide production |
| dc.title.none.fl_str_mv | Bacillus thuringiensis strain QBT220 pBtoxis plasmid structural instability enhances δ-endotoxins synthesis and bioinsecticidal activity |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr"><i>Bacillus thuringiensis</i> subsp. <i>israelensis</i> (<i>Bti</i>) spherical parasporal crystal contains several insecticidal proteins used as environmentally safe alternative to toxic chemical pesticides. The exploration of a <i>Bti</i> strain isolated from Qatar QBT220 genes encoding the δ-endotoxins responsible of the insecticidal activities revealed the alteration of a 14-kb DNA region including the δ-endotoxins <i>cry10A</i> and <i>cyt1C</i> genes of pBtoxis plasmid. The presence of all the insecticidal genes except <i>cry10A</i> and <i>cyt1C</i> was explained by a structural instability of the plasmid pBtoxis. However, when compared with the <i>Bti</i> reference strains H14 and QBT217 that carry all δ-endotoxins coding genes, it was found that QBT220, has a significantly higher insecticidal activity against the dipteran insect <i>Aedes aegypti</i> larvae despite of the plasmid pBtoxis structural instability due to the alteration of <i>cry10A</i> and <i>cyt1C</i> genes. In addition, QBT220 showed the highest δ-endotoxin synthesis per spore, compared with that of the wildtype strains. These findings confirm that the altered genes <i>cry10A</i> and <i>cyt1C</i> are not mandatory for <i>Bti</i> insecticidal activities and on the other hand show a possible inhibitory effect played by the 2 proteins Cry10A and Cyt1C on the insecticidal activities of the other insecticidal proteins. In addition, the QBT220 increased δ-endotoxins synthesis per cell, makes this strain a good candidate for possible applications in the industrial production of bioinsecticides.</p><h2>Other Information</h2><p dir="ltr">Published in: Ecotoxicology and Environmental Safety<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.ecoenv.2021.112975" target="_blank">https://dx.doi.org/10.1016/j.ecoenv.2021.112975</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_5444c1e6b02475c5d32124cde82b8b36 |
| identifier_str_mv | 10.1016/j.ecoenv.2021.112975 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/24314338 |
| publishDate | 2021 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Bacillus thuringiensis strain QBT220 pBtoxis plasmid structural instability enhances δ-endotoxins synthesis and bioinsecticidal activityKavita Nair (17151127)Roda Al-Thani (540907)Samir Jaoua (11624805)Biomedical and clinical sciencesMedical microbiologyPharmacology and pharmaceutical sciencesEnvironmental sciencesEnvironmental managementBacillus thuringiensisPBtoxis plasmid instabilityBioinsecticide production<p dir="ltr"><i>Bacillus thuringiensis</i> subsp. <i>israelensis</i> (<i>Bti</i>) spherical parasporal crystal contains several insecticidal proteins used as environmentally safe alternative to toxic chemical pesticides. The exploration of a <i>Bti</i> strain isolated from Qatar QBT220 genes encoding the δ-endotoxins responsible of the insecticidal activities revealed the alteration of a 14-kb DNA region including the δ-endotoxins <i>cry10A</i> and <i>cyt1C</i> genes of pBtoxis plasmid. The presence of all the insecticidal genes except <i>cry10A</i> and <i>cyt1C</i> was explained by a structural instability of the plasmid pBtoxis. However, when compared with the <i>Bti</i> reference strains H14 and QBT217 that carry all δ-endotoxins coding genes, it was found that QBT220, has a significantly higher insecticidal activity against the dipteran insect <i>Aedes aegypti</i> larvae despite of the plasmid pBtoxis structural instability due to the alteration of <i>cry10A</i> and <i>cyt1C</i> genes. In addition, QBT220 showed the highest δ-endotoxin synthesis per spore, compared with that of the wildtype strains. These findings confirm that the altered genes <i>cry10A</i> and <i>cyt1C</i> are not mandatory for <i>Bti</i> insecticidal activities and on the other hand show a possible inhibitory effect played by the 2 proteins Cry10A and Cyt1C on the insecticidal activities of the other insecticidal proteins. In addition, the QBT220 increased δ-endotoxins synthesis per cell, makes this strain a good candidate for possible applications in the industrial production of bioinsecticides.</p><h2>Other Information</h2><p dir="ltr">Published in: Ecotoxicology and Environmental Safety<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.ecoenv.2021.112975" target="_blank">https://dx.doi.org/10.1016/j.ecoenv.2021.112975</a></p>2021-12-25T00:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1016/j.ecoenv.2021.112975https://figshare.com/articles/journal_contribution/Bacillus_thuringiensis_strain_QBT220_pBtoxis_plasmid_structural_instability_enhances_-endotoxins_synthesis_and_bioinsecticidal_activity/24314338CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/243143382021-12-25T00:00:00Z |
| spellingShingle | Bacillus thuringiensis strain QBT220 pBtoxis plasmid structural instability enhances δ-endotoxins synthesis and bioinsecticidal activity Kavita Nair (17151127) Biomedical and clinical sciences Medical microbiology Pharmacology and pharmaceutical sciences Environmental sciences Environmental management Bacillus thuringiensis PBtoxis plasmid instability Bioinsecticide production |
| status_str | publishedVersion |
| title | Bacillus thuringiensis strain QBT220 pBtoxis plasmid structural instability enhances δ-endotoxins synthesis and bioinsecticidal activity |
| title_full | Bacillus thuringiensis strain QBT220 pBtoxis plasmid structural instability enhances δ-endotoxins synthesis and bioinsecticidal activity |
| title_fullStr | Bacillus thuringiensis strain QBT220 pBtoxis plasmid structural instability enhances δ-endotoxins synthesis and bioinsecticidal activity |
| title_full_unstemmed | Bacillus thuringiensis strain QBT220 pBtoxis plasmid structural instability enhances δ-endotoxins synthesis and bioinsecticidal activity |
| title_short | Bacillus thuringiensis strain QBT220 pBtoxis plasmid structural instability enhances δ-endotoxins synthesis and bioinsecticidal activity |
| title_sort | Bacillus thuringiensis strain QBT220 pBtoxis plasmid structural instability enhances δ-endotoxins synthesis and bioinsecticidal activity |
| topic | Biomedical and clinical sciences Medical microbiology Pharmacology and pharmaceutical sciences Environmental sciences Environmental management Bacillus thuringiensis PBtoxis plasmid instability Bioinsecticide production |