Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2
<p dir="ltr">The recent outbreak of the Coronavirus disease 2019 (COVID-19) has quickly spread worldwide since its discovery in Wuhan city, China in December 2019. A comprehensive strategy, including surveillance, diagnostics, research, clinical treatment, and development of vaccines...
محفوظ في:
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| مؤلفون آخرون: | , , , , , , |
| منشور في: |
2022
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إضافة وسم
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| _version_ | 1864513563380416512 |
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| author | Nadin Younes (4863280) |
| author2 | Duaa W. Al-Sadeq (16515090) Hadeel AL-Jighefee (16515093) Salma Younes (6424865) Ola Al-Jamal (11721377) Hanin I. Daas (16515095) Hadi. M. Yassine (16515098) Gheyath K. Nasrallah (2182598) |
| author2_role | author author author author author author author |
| author_facet | Nadin Younes (4863280) Duaa W. Al-Sadeq (16515090) Hadeel AL-Jighefee (16515093) Salma Younes (6424865) Ola Al-Jamal (11721377) Hanin I. Daas (16515095) Hadi. M. Yassine (16515098) Gheyath K. Nasrallah (2182598) |
| author_role | author |
| dc.creator.none.fl_str_mv | Nadin Younes (4863280) Duaa W. Al-Sadeq (16515090) Hadeel AL-Jighefee (16515093) Salma Younes (6424865) Ola Al-Jamal (11721377) Hanin I. Daas (16515095) Hadi. M. Yassine (16515098) Gheyath K. Nasrallah (2182598) |
| dc.date.none.fl_str_mv | 2022-05-26T00:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3390/v12060582 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Challenges_in_Laboratory_Diagnosis_of_the_Novel_Coronavirus_SARS-CoV-2/23650332 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Microbiology Biomedical and clinical sciences Clinical sciences COVID-19 SARS-CoV-2 viruses diagnostic challenges molecular testing serology |
| dc.title.none.fl_str_mv | Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2 |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">The recent outbreak of the Coronavirus disease 2019 (COVID-19) has quickly spread worldwide since its discovery in Wuhan city, China in December 2019. A comprehensive strategy, including surveillance, diagnostics, research, clinical treatment, and development of vaccines, is urgently needed to win the battle against COVID-19. The past three unprecedented outbreaks of emerging human coronavirus infections at the beginning of the 21st century have highlighted the importance of readily available, accurate, and rapid diagnostic technologies to contain emerging and re-emerging pandemics. Real-time reverse transcriptase-polymerase chain reaction (rRT-PCR) based assays performed on respiratory specimens remain the gold standard for COVID-19 diagnostics. However, point-of-care technologies and serologic immunoassays are rapidly emerging with high sensitivity and specificity as well. Even though excellent techniques are available for the diagnosis of symptomatic patients with COVID-19 in well-equipped laboratories; critical gaps still remain in screening asymptomatic people who are in the incubation phase of the virus, as well as in the accurate determination of live viral shedding during convalescence to inform decisions for ending isolation. This review article aims to discuss the currently available laboratory methods and surveillance technologies available for the detection of COVID-19, their performance characteristics and highlight the gaps in current diagnostic capacity, and finally, propose potential solutions. We also summarize the specifications of the majority of the available commercial kits (PCR, EIA, and POC) for laboratory diagnosis of COVID-19.</p><h2>Other Information</h2><p dir="ltr">Published in: Viruses<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://doi.org/10.3390/v12060582" target="_blank">https://doi.org/10.3390/v12060582</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_5588da241634321f2f27f2707c1fd712 |
| identifier_str_mv | 10.3390/v12060582 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/23650332 |
| publishDate | 2022 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2Nadin Younes (4863280)Duaa W. Al-Sadeq (16515090)Hadeel AL-Jighefee (16515093)Salma Younes (6424865)Ola Al-Jamal (11721377)Hanin I. Daas (16515095)Hadi. M. Yassine (16515098)Gheyath K. Nasrallah (2182598)Biological sciencesMicrobiologyBiomedical and clinical sciencesClinical sciencesCOVID-19SARS-CoV-2virusesdiagnostic challengesmolecular testingserology<p dir="ltr">The recent outbreak of the Coronavirus disease 2019 (COVID-19) has quickly spread worldwide since its discovery in Wuhan city, China in December 2019. A comprehensive strategy, including surveillance, diagnostics, research, clinical treatment, and development of vaccines, is urgently needed to win the battle against COVID-19. The past three unprecedented outbreaks of emerging human coronavirus infections at the beginning of the 21st century have highlighted the importance of readily available, accurate, and rapid diagnostic technologies to contain emerging and re-emerging pandemics. Real-time reverse transcriptase-polymerase chain reaction (rRT-PCR) based assays performed on respiratory specimens remain the gold standard for COVID-19 diagnostics. However, point-of-care technologies and serologic immunoassays are rapidly emerging with high sensitivity and specificity as well. Even though excellent techniques are available for the diagnosis of symptomatic patients with COVID-19 in well-equipped laboratories; critical gaps still remain in screening asymptomatic people who are in the incubation phase of the virus, as well as in the accurate determination of live viral shedding during convalescence to inform decisions for ending isolation. This review article aims to discuss the currently available laboratory methods and surveillance technologies available for the detection of COVID-19, their performance characteristics and highlight the gaps in current diagnostic capacity, and finally, propose potential solutions. We also summarize the specifications of the majority of the available commercial kits (PCR, EIA, and POC) for laboratory diagnosis of COVID-19.</p><h2>Other Information</h2><p dir="ltr">Published in: Viruses<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://doi.org/10.3390/v12060582" target="_blank">https://doi.org/10.3390/v12060582</a></p>2022-05-26T00:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3390/v12060582https://figshare.com/articles/journal_contribution/Challenges_in_Laboratory_Diagnosis_of_the_Novel_Coronavirus_SARS-CoV-2/23650332CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/236503322022-05-26T00:00:00Z |
| spellingShingle | Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2 Nadin Younes (4863280) Biological sciences Microbiology Biomedical and clinical sciences Clinical sciences COVID-19 SARS-CoV-2 viruses diagnostic challenges molecular testing serology |
| status_str | publishedVersion |
| title | Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2 |
| title_full | Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2 |
| title_fullStr | Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2 |
| title_full_unstemmed | Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2 |
| title_short | Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2 |
| title_sort | Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2 |
| topic | Biological sciences Microbiology Biomedical and clinical sciences Clinical sciences COVID-19 SARS-CoV-2 viruses diagnostic challenges molecular testing serology |