Diagnostic Efficiency of Three Fully Automated Serology Assays and Their Correlation with a Novel Surrogate Virus Neutralization Test in Symptomatic and Asymptomatic SARS-COV-2 Individuals
<p dir="ltr">To support the deployment of serology assays for population screening during the COVID-19 pandemic, we compared the performance of three fully automated SARS-CoV-2 IgG assays: Mindray CL-900i® (target: spike [S] and nucleocapsid [N]), BioMérieux VIDAS®3 (target: receptor...
محفوظ في:
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| مؤلفون آخرون: | , , , , , , , , , , , , |
| منشور في: |
2021
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إضافة وسم
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| _version_ | 1864513516712493056 |
|---|---|
| author | Salma Younes (6424865) |
| author2 | Hadeel Al-Jighefee (17128738) Farah Shurrab (11079708) Duaa W. Al-Sadeq (10976754) Nadin Younes (4863280) Soha R. Dargham (3613250) Nader Al-Dewik (4166527) Hamda Qotba (12794976) Mohamed Syed (5327276) Ahmed Alnuaimi (18513774) Hadi M. Yassine (4675846) Patrick Tang (239534) Laith J. Abu-Raddad (9262524) Gheyath K. Nasrallah (9200525) |
| author2_role | author author author author author author author author author author author author author |
| author_facet | Salma Younes (6424865) Hadeel Al-Jighefee (17128738) Farah Shurrab (11079708) Duaa W. Al-Sadeq (10976754) Nadin Younes (4863280) Soha R. Dargham (3613250) Nader Al-Dewik (4166527) Hamda Qotba (12794976) Mohamed Syed (5327276) Ahmed Alnuaimi (18513774) Hadi M. Yassine (4675846) Patrick Tang (239534) Laith J. Abu-Raddad (9262524) Gheyath K. Nasrallah (9200525) |
| author_role | author |
| dc.creator.none.fl_str_mv | Salma Younes (6424865) Hadeel Al-Jighefee (17128738) Farah Shurrab (11079708) Duaa W. Al-Sadeq (10976754) Nadin Younes (4863280) Soha R. Dargham (3613250) Nader Al-Dewik (4166527) Hamda Qotba (12794976) Mohamed Syed (5327276) Ahmed Alnuaimi (18513774) Hadi M. Yassine (4675846) Patrick Tang (239534) Laith J. Abu-Raddad (9262524) Gheyath K. Nasrallah (9200525) |
| dc.date.none.fl_str_mv | 2021-01-25T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3390/microorganisms9020245 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Diagnostic_Efficiency_of_Three_Fully_Automated_Serology_Assays_and_Their_Correlation_with_a_Novel_Surrogate_Virus_Neutralization_Test_in_Symptomatic_and_Asymptomatic_SARS-COV-2_Individuals/25764870 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Clinical sciences Immunology COVID-19 SARS-CoV-2 serology sensitivity specificity neutralizing antibodies surrogate virus neutralization test (sVNT) |
| dc.title.none.fl_str_mv | Diagnostic Efficiency of Three Fully Automated Serology Assays and Their Correlation with a Novel Surrogate Virus Neutralization Test in Symptomatic and Asymptomatic SARS-COV-2 Individuals |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">To support the deployment of serology assays for population screening during the COVID-19 pandemic, we compared the performance of three fully automated SARS-CoV-2 IgG assays: Mindray CL-900i® (target: spike [S] and nucleocapsid [N]), BioMérieux VIDAS®3 (target: receptor-binding domain [RBD]) and Diasorin LIAISON®XL (target: S1 and S2 subunits). A total of 111 SARS-CoV-2 RT-PCR- positive samples collected at ≥ 21 days post symptom onset, and 127 pre-pandemic control samples were included. Diagnostic performance was assessed in correlation to RT-PCR and a surrogate virus-neutralizing test (sVNT). Moreover, cross-reactivity with other viral antibodies was investigated. Compared to RT-PCR, LIAISON®XL showed the highest overall specificity (100%), followed by VIDAS®3 (98.4%) and CL-900i® (95.3%). The highest sensitivity was demonstrated by CL-900i® (90.1%), followed by VIDAS®3 (88.3%) and LIAISON®XL (85.6%). The sensitivity of all assays was higher in symptomatic patients (91.1–98.2%) compared to asymptomatic patients (78.4–80.4%). In correlation to sVNT, all assays showed excellent sensitivities (92.2–96.1%). In addition, VIDAS®3 demonstrated the best correlation (r = 0.75) with the sVNT. The present study provides insights on the performance of three fully automated assays, which could help diagnostic laboratories in the choice of a particular assay according to the intended use.</p><p><br></p><h2>Other Information</h2><p dir="ltr">Published in: Microorganisms<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/microorganisms9020245" target="_blank">https://dx.doi.org/10.3390/microorganisms9020245</a></p><p dir="ltr">Additional institutions affiliated with: Primary Health Care Corporation, Qatar Medical Center, Women's Wellness and Research Center - HMC<br><br></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_57401a4b2247229d7e9bd9a1ed70d6c2 |
| identifier_str_mv | 10.3390/microorganisms9020245 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25764870 |
| publishDate | 2021 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Diagnostic Efficiency of Three Fully Automated Serology Assays and Their Correlation with a Novel Surrogate Virus Neutralization Test in Symptomatic and Asymptomatic SARS-COV-2 IndividualsSalma Younes (6424865)Hadeel Al-Jighefee (17128738)Farah Shurrab (11079708)Duaa W. Al-Sadeq (10976754)Nadin Younes (4863280)Soha R. Dargham (3613250)Nader Al-Dewik (4166527)Hamda Qotba (12794976)Mohamed Syed (5327276)Ahmed Alnuaimi (18513774)Hadi M. Yassine (4675846)Patrick Tang (239534)Laith J. Abu-Raddad (9262524)Gheyath K. Nasrallah (9200525)Biomedical and clinical sciencesClinical sciencesImmunologyCOVID-19SARS-CoV-2serologysensitivityspecificityneutralizing antibodiessurrogate virus neutralization test (sVNT)<p dir="ltr">To support the deployment of serology assays for population screening during the COVID-19 pandemic, we compared the performance of three fully automated SARS-CoV-2 IgG assays: Mindray CL-900i® (target: spike [S] and nucleocapsid [N]), BioMérieux VIDAS®3 (target: receptor-binding domain [RBD]) and Diasorin LIAISON®XL (target: S1 and S2 subunits). A total of 111 SARS-CoV-2 RT-PCR- positive samples collected at ≥ 21 days post symptom onset, and 127 pre-pandemic control samples were included. Diagnostic performance was assessed in correlation to RT-PCR and a surrogate virus-neutralizing test (sVNT). Moreover, cross-reactivity with other viral antibodies was investigated. Compared to RT-PCR, LIAISON®XL showed the highest overall specificity (100%), followed by VIDAS®3 (98.4%) and CL-900i® (95.3%). The highest sensitivity was demonstrated by CL-900i® (90.1%), followed by VIDAS®3 (88.3%) and LIAISON®XL (85.6%). The sensitivity of all assays was higher in symptomatic patients (91.1–98.2%) compared to asymptomatic patients (78.4–80.4%). In correlation to sVNT, all assays showed excellent sensitivities (92.2–96.1%). In addition, VIDAS®3 demonstrated the best correlation (r = 0.75) with the sVNT. The present study provides insights on the performance of three fully automated assays, which could help diagnostic laboratories in the choice of a particular assay according to the intended use.</p><p><br></p><h2>Other Information</h2><p dir="ltr">Published in: Microorganisms<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/microorganisms9020245" target="_blank">https://dx.doi.org/10.3390/microorganisms9020245</a></p><p dir="ltr">Additional institutions affiliated with: Primary Health Care Corporation, Qatar Medical Center, Women's Wellness and Research Center - HMC<br><br></p>2021-01-25T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3390/microorganisms9020245https://figshare.com/articles/journal_contribution/Diagnostic_Efficiency_of_Three_Fully_Automated_Serology_Assays_and_Their_Correlation_with_a_Novel_Surrogate_Virus_Neutralization_Test_in_Symptomatic_and_Asymptomatic_SARS-COV-2_Individuals/25764870CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/257648702021-01-25T03:00:00Z |
| spellingShingle | Diagnostic Efficiency of Three Fully Automated Serology Assays and Their Correlation with a Novel Surrogate Virus Neutralization Test in Symptomatic and Asymptomatic SARS-COV-2 Individuals Salma Younes (6424865) Biomedical and clinical sciences Clinical sciences Immunology COVID-19 SARS-CoV-2 serology sensitivity specificity neutralizing antibodies surrogate virus neutralization test (sVNT) |
| status_str | publishedVersion |
| title | Diagnostic Efficiency of Three Fully Automated Serology Assays and Their Correlation with a Novel Surrogate Virus Neutralization Test in Symptomatic and Asymptomatic SARS-COV-2 Individuals |
| title_full | Diagnostic Efficiency of Three Fully Automated Serology Assays and Their Correlation with a Novel Surrogate Virus Neutralization Test in Symptomatic and Asymptomatic SARS-COV-2 Individuals |
| title_fullStr | Diagnostic Efficiency of Three Fully Automated Serology Assays and Their Correlation with a Novel Surrogate Virus Neutralization Test in Symptomatic and Asymptomatic SARS-COV-2 Individuals |
| title_full_unstemmed | Diagnostic Efficiency of Three Fully Automated Serology Assays and Their Correlation with a Novel Surrogate Virus Neutralization Test in Symptomatic and Asymptomatic SARS-COV-2 Individuals |
| title_short | Diagnostic Efficiency of Three Fully Automated Serology Assays and Their Correlation with a Novel Surrogate Virus Neutralization Test in Symptomatic and Asymptomatic SARS-COV-2 Individuals |
| title_sort | Diagnostic Efficiency of Three Fully Automated Serology Assays and Their Correlation with a Novel Surrogate Virus Neutralization Test in Symptomatic and Asymptomatic SARS-COV-2 Individuals |
| topic | Biomedical and clinical sciences Clinical sciences Immunology COVID-19 SARS-CoV-2 serology sensitivity specificity neutralizing antibodies surrogate virus neutralization test (sVNT) |