RNA-Seq Analysis of Colorectal Tumor-Infiltrating Myeloid-Derived Suppressor Cell Subsets Revealed Gene Signatures of Poor Prognosis
<p dir="ltr">Elevated levels of myeloid-derived suppressor cells (MDSCs), including polymorphonuclear MDSCs (PMN-MDSCs) and immature MDSCs (I-MDSCs), are usually associated with disease progression in cancer patients, including colorectal cancer (CRC). However, biological mechanisms...
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2020
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| _version_ | 1864513512817033216 |
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| author | Reem Saleh (3513056) |
| author2 | Varun Sasidharan Nair (5396393) Mahmood Al-Dhaheri (9621035) Mahwish Khawar (9621038) Mohamed Abu Nada (8309784) Nehad M. Alajez (7397276) Eyad Elkord (5396390) |
| author2_role | author author author author author author |
| author_facet | Reem Saleh (3513056) Varun Sasidharan Nair (5396393) Mahmood Al-Dhaheri (9621035) Mahwish Khawar (9621038) Mohamed Abu Nada (8309784) Nehad M. Alajez (7397276) Eyad Elkord (5396390) |
| author_role | author |
| dc.creator.none.fl_str_mv | Reem Saleh (3513056) Varun Sasidharan Nair (5396393) Mahmood Al-Dhaheri (9621035) Mahwish Khawar (9621038) Mohamed Abu Nada (8309784) Nehad M. Alajez (7397276) Eyad Elkord (5396390) |
| dc.date.none.fl_str_mv | 2020-11-10T09:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3389/fonc.2020.604906 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/RNA-Seq_Analysis_of_Colorectal_Tumor-Infiltrating_Myeloid-Derived_Suppressor_Cell_Subsets_Revealed_Gene_Signatures_of_Poor_Prognosis/25957969 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Genetics Biomedical and clinical sciences Immunology Medical biochemistry and metabolomics Oncology and carcinogenesis myeloid-derived suppressor cells colorectal cancer transcriptomic profiling metabolism signaling pathways immune responses |
| dc.title.none.fl_str_mv | RNA-Seq Analysis of Colorectal Tumor-Infiltrating Myeloid-Derived Suppressor Cell Subsets Revealed Gene Signatures of Poor Prognosis |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Elevated levels of myeloid-derived suppressor cells (MDSCs), including polymorphonuclear MDSCs (PMN-MDSCs) and immature MDSCs (I-MDSCs), are usually associated with disease progression in cancer patients, including colorectal cancer (CRC). However, biological mechanisms and molecular pathways regulated by MDSC subpopulations in the CRC tumor microenvironment (TME) have not been fully investigated. In this study, we performed transcriptomic analysis of tumor-infiltrating I-MDSCs and PMN-MDSCs isolated from tumor tissues of six CRC patients, compared to antigen-presenting cells (APCs). We also compared the transcriptomic profiles of tumor-infiltrating PMN-MDSCs to I-MDSCs. Our results showed different molecular pathways regulated by each MDSC subset, potentially reflecting their phenotypical/molecular/functional characteristics in the CRC TME. Moreover, we identified gene signatures in PMN-MDSC and I-MDSC of poor overall survival (OS) and disease-free survival (DFS) using the Cancer Genome Atlas (TCGA) dataset from patients with colon adenocarcinoma (COAD). However, functional studies are required to validate these findings.</p><h2>Other Information</h2><p dir="ltr">Published in: Frontiers in Oncology<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3389/fonc.2020.604906" target="_blank">https://dx.doi.org/10.3389/fonc.2020.604906</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_59b6b438976b4ab0f0b424019dbb550a |
| identifier_str_mv | 10.3389/fonc.2020.604906 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25957969 |
| publishDate | 2020 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | RNA-Seq Analysis of Colorectal Tumor-Infiltrating Myeloid-Derived Suppressor Cell Subsets Revealed Gene Signatures of Poor PrognosisReem Saleh (3513056)Varun Sasidharan Nair (5396393)Mahmood Al-Dhaheri (9621035)Mahwish Khawar (9621038)Mohamed Abu Nada (8309784)Nehad M. Alajez (7397276)Eyad Elkord (5396390)Biological sciencesGeneticsBiomedical and clinical sciencesImmunologyMedical biochemistry and metabolomicsOncology and carcinogenesismyeloid-derived suppressor cellscolorectal cancertranscriptomic profilingmetabolismsignaling pathwaysimmune responses<p dir="ltr">Elevated levels of myeloid-derived suppressor cells (MDSCs), including polymorphonuclear MDSCs (PMN-MDSCs) and immature MDSCs (I-MDSCs), are usually associated with disease progression in cancer patients, including colorectal cancer (CRC). However, biological mechanisms and molecular pathways regulated by MDSC subpopulations in the CRC tumor microenvironment (TME) have not been fully investigated. In this study, we performed transcriptomic analysis of tumor-infiltrating I-MDSCs and PMN-MDSCs isolated from tumor tissues of six CRC patients, compared to antigen-presenting cells (APCs). We also compared the transcriptomic profiles of tumor-infiltrating PMN-MDSCs to I-MDSCs. Our results showed different molecular pathways regulated by each MDSC subset, potentially reflecting their phenotypical/molecular/functional characteristics in the CRC TME. Moreover, we identified gene signatures in PMN-MDSC and I-MDSC of poor overall survival (OS) and disease-free survival (DFS) using the Cancer Genome Atlas (TCGA) dataset from patients with colon adenocarcinoma (COAD). However, functional studies are required to validate these findings.</p><h2>Other Information</h2><p dir="ltr">Published in: Frontiers in Oncology<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3389/fonc.2020.604906" target="_blank">https://dx.doi.org/10.3389/fonc.2020.604906</a></p>2020-11-10T09:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3389/fonc.2020.604906https://figshare.com/articles/journal_contribution/RNA-Seq_Analysis_of_Colorectal_Tumor-Infiltrating_Myeloid-Derived_Suppressor_Cell_Subsets_Revealed_Gene_Signatures_of_Poor_Prognosis/25957969CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/259579692020-11-10T09:00:00Z |
| spellingShingle | RNA-Seq Analysis of Colorectal Tumor-Infiltrating Myeloid-Derived Suppressor Cell Subsets Revealed Gene Signatures of Poor Prognosis Reem Saleh (3513056) Biological sciences Genetics Biomedical and clinical sciences Immunology Medical biochemistry and metabolomics Oncology and carcinogenesis myeloid-derived suppressor cells colorectal cancer transcriptomic profiling metabolism signaling pathways immune responses |
| status_str | publishedVersion |
| title | RNA-Seq Analysis of Colorectal Tumor-Infiltrating Myeloid-Derived Suppressor Cell Subsets Revealed Gene Signatures of Poor Prognosis |
| title_full | RNA-Seq Analysis of Colorectal Tumor-Infiltrating Myeloid-Derived Suppressor Cell Subsets Revealed Gene Signatures of Poor Prognosis |
| title_fullStr | RNA-Seq Analysis of Colorectal Tumor-Infiltrating Myeloid-Derived Suppressor Cell Subsets Revealed Gene Signatures of Poor Prognosis |
| title_full_unstemmed | RNA-Seq Analysis of Colorectal Tumor-Infiltrating Myeloid-Derived Suppressor Cell Subsets Revealed Gene Signatures of Poor Prognosis |
| title_short | RNA-Seq Analysis of Colorectal Tumor-Infiltrating Myeloid-Derived Suppressor Cell Subsets Revealed Gene Signatures of Poor Prognosis |
| title_sort | RNA-Seq Analysis of Colorectal Tumor-Infiltrating Myeloid-Derived Suppressor Cell Subsets Revealed Gene Signatures of Poor Prognosis |
| topic | Biological sciences Genetics Biomedical and clinical sciences Immunology Medical biochemistry and metabolomics Oncology and carcinogenesis myeloid-derived suppressor cells colorectal cancer transcriptomic profiling metabolism signaling pathways immune responses |