Immunohistochemical profiling of the heat shock response in obese non-diabetic subjects revealed impaired expression of heat shock proteins in the adipose tissue
<p dir="ltr">Obesity is characterized by a chronic low-grade inflammation and altered stress responses in key metabolic tissues. Impairment of heat shock response (HSR) has been already linked to diabetes and insulin resistance as reflected by decrease in heat shock proteins (HSPs) e...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , , , , , , , , , , , |
| منشور في: |
2014
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| الموضوعات: | |
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إضافة وسم
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| _version_ | 1864513506541305856 |
|---|---|
| author | Ali Tiss (437068) |
| author2 | Abdelkrim Khadir (437074) Jehad Abubaker (437067) Mohamed Abu-Farha (25322) Irina Al-Khairi (437069) Preethi Cherian (437070) Jeena John (437072) Sina Kavalakatt (437073) Samia Warsame (437075) Fahad Al-Ghimlas (412125) Naser Elkum (79418) Kazem Behbehani (412126) Said Dermime (79420) Mohammed Dehbi (309033) |
| author2_role | author author author author author author author author author author author author author |
| author_facet | Ali Tiss (437068) Abdelkrim Khadir (437074) Jehad Abubaker (437067) Mohamed Abu-Farha (25322) Irina Al-Khairi (437069) Preethi Cherian (437070) Jeena John (437072) Sina Kavalakatt (437073) Samia Warsame (437075) Fahad Al-Ghimlas (412125) Naser Elkum (79418) Kazem Behbehani (412126) Said Dermime (79420) Mohammed Dehbi (309033) |
| author_role | author |
| dc.creator.none.fl_str_mv | Ali Tiss (437068) Abdelkrim Khadir (437074) Jehad Abubaker (437067) Mohamed Abu-Farha (25322) Irina Al-Khairi (437069) Preethi Cherian (437070) Jeena John (437072) Sina Kavalakatt (437073) Samia Warsame (437075) Fahad Al-Ghimlas (412125) Naser Elkum (79418) Kazem Behbehani (412126) Said Dermime (79420) Mohammed Dehbi (309033) |
| dc.date.none.fl_str_mv | 2014-07-01T00:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1186/1476-511x-13-106 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Immunohistochemical_profiling_of_the_heat_shock_response_in_obese_non-diabetic_subjects_revealed_impaired_expression_of_heat_shock_proteins_in_the_adipose_tissue/26892238 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Medical biochemistry and metabolomics Health sciences Sports science and exercise Heat shock protein HSP Exercise ER stress Obesity Adipose tissue |
| dc.title.none.fl_str_mv | Immunohistochemical profiling of the heat shock response in obese non-diabetic subjects revealed impaired expression of heat shock proteins in the adipose tissue |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Obesity is characterized by a chronic low-grade inflammation and altered stress responses in key metabolic tissues. Impairment of heat shock response (HSR) has been already linked to diabetes and insulin resistance as reflected by decrease in heat shock proteins (HSPs) expression. However, the status of HSR in non-diabetic human obese has not yet been elucidated. The aim of the current study was to investigate whether obesity triggers a change in the HSR pattern and the impact of physical exercise on this pattern at protein and mRNA levels. Two groups of adult non-diabetic human subjects consisting of lean and obese (n = 47 for each group) were enrolled in this study. The expression pattern of HSP-27, DNAJB3/HSP-40, HSP-60, HSC-70, HSP72, HSP-90 and GRP-94 in the adipose tissue was primarily investigated by immunohistochemistry and then complemented by western blot and qRT-PCR in Peripheral blood mononuclear cells (PBMCs). HSPs expression levels were correlated with various physical, clinical and biochemical parameters. We have also explored the effect of a 3-month moderate physical exercise on the HSPs expression pattern in obese subjects. Obese subjects displayed increased expression of HSP-60, HSC-70, HSP-72, HSP-90 and GRP-94 and lower expression of DNAJB3/HSP-40 (P < 0.05). No differential expression was observed for HSP-27 between the two groups. Higher levels of HSP-72 and GRP-94 proteins correlated positively with the indices of obesity (body mass index and percent body fat) and circulating levels of IFN-gamma-inducible protein 10 (IP-10) and RANTES chemokines. This expression pattern was concomitant with increased inflammatory response in the adipose tissue as monitored by increased levels of Interleukin-6 (IL-6), Tumor necrosis factor-α (TNF-α), and RANTES (P < 0.05). Physical exercise reduced the expression of various HSPs in obese to normal levels observed in lean subjects with a parallel decrease in the endogenous levels of IL-6, TNF-α, and RANTES. Taken together, these data indicate that obesity triggers differential regulation of various components of the HSR in non-diabetic subjects and a 3-month physical moderate exercise was sufficient to restore the normal expression of HSPs in the adipose tissue with concomitant attenuation in the inflammatory response.</p><h2>Other Information</h2><p dir="ltr">Published in: Lipids in Health and Disease<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" rel="license" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1186/1476-511x-13-106" target="_blank">https://dx.doi.org/10.1186/1476-511x-13-106</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_5d02ad16defa40d29c5bf09463f6e161 |
| identifier_str_mv | 10.1186/1476-511x-13-106 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/26892238 |
| publishDate | 2014 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Immunohistochemical profiling of the heat shock response in obese non-diabetic subjects revealed impaired expression of heat shock proteins in the adipose tissueAli Tiss (437068)Abdelkrim Khadir (437074)Jehad Abubaker (437067)Mohamed Abu-Farha (25322)Irina Al-Khairi (437069)Preethi Cherian (437070)Jeena John (437072)Sina Kavalakatt (437073)Samia Warsame (437075)Fahad Al-Ghimlas (412125)Naser Elkum (79418)Kazem Behbehani (412126)Said Dermime (79420)Mohammed Dehbi (309033)Biomedical and clinical sciencesMedical biochemistry and metabolomicsHealth sciencesSports science and exerciseHeat shock proteinHSPExerciseER stressObesityAdipose tissue<p dir="ltr">Obesity is characterized by a chronic low-grade inflammation and altered stress responses in key metabolic tissues. Impairment of heat shock response (HSR) has been already linked to diabetes and insulin resistance as reflected by decrease in heat shock proteins (HSPs) expression. However, the status of HSR in non-diabetic human obese has not yet been elucidated. The aim of the current study was to investigate whether obesity triggers a change in the HSR pattern and the impact of physical exercise on this pattern at protein and mRNA levels. Two groups of adult non-diabetic human subjects consisting of lean and obese (n = 47 for each group) were enrolled in this study. The expression pattern of HSP-27, DNAJB3/HSP-40, HSP-60, HSC-70, HSP72, HSP-90 and GRP-94 in the adipose tissue was primarily investigated by immunohistochemistry and then complemented by western blot and qRT-PCR in Peripheral blood mononuclear cells (PBMCs). HSPs expression levels were correlated with various physical, clinical and biochemical parameters. We have also explored the effect of a 3-month moderate physical exercise on the HSPs expression pattern in obese subjects. Obese subjects displayed increased expression of HSP-60, HSC-70, HSP-72, HSP-90 and GRP-94 and lower expression of DNAJB3/HSP-40 (P < 0.05). No differential expression was observed for HSP-27 between the two groups. Higher levels of HSP-72 and GRP-94 proteins correlated positively with the indices of obesity (body mass index and percent body fat) and circulating levels of IFN-gamma-inducible protein 10 (IP-10) and RANTES chemokines. This expression pattern was concomitant with increased inflammatory response in the adipose tissue as monitored by increased levels of Interleukin-6 (IL-6), Tumor necrosis factor-α (TNF-α), and RANTES (P < 0.05). Physical exercise reduced the expression of various HSPs in obese to normal levels observed in lean subjects with a parallel decrease in the endogenous levels of IL-6, TNF-α, and RANTES. Taken together, these data indicate that obesity triggers differential regulation of various components of the HSR in non-diabetic subjects and a 3-month physical moderate exercise was sufficient to restore the normal expression of HSPs in the adipose tissue with concomitant attenuation in the inflammatory response.</p><h2>Other Information</h2><p dir="ltr">Published in: Lipids in Health and Disease<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" rel="license" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1186/1476-511x-13-106" target="_blank">https://dx.doi.org/10.1186/1476-511x-13-106</a></p>2014-07-01T00:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1186/1476-511x-13-106https://figshare.com/articles/journal_contribution/Immunohistochemical_profiling_of_the_heat_shock_response_in_obese_non-diabetic_subjects_revealed_impaired_expression_of_heat_shock_proteins_in_the_adipose_tissue/26892238CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/268922382014-07-01T00:00:00Z |
| spellingShingle | Immunohistochemical profiling of the heat shock response in obese non-diabetic subjects revealed impaired expression of heat shock proteins in the adipose tissue Ali Tiss (437068) Biomedical and clinical sciences Medical biochemistry and metabolomics Health sciences Sports science and exercise Heat shock protein HSP Exercise ER stress Obesity Adipose tissue |
| status_str | publishedVersion |
| title | Immunohistochemical profiling of the heat shock response in obese non-diabetic subjects revealed impaired expression of heat shock proteins in the adipose tissue |
| title_full | Immunohistochemical profiling of the heat shock response in obese non-diabetic subjects revealed impaired expression of heat shock proteins in the adipose tissue |
| title_fullStr | Immunohistochemical profiling of the heat shock response in obese non-diabetic subjects revealed impaired expression of heat shock proteins in the adipose tissue |
| title_full_unstemmed | Immunohistochemical profiling of the heat shock response in obese non-diabetic subjects revealed impaired expression of heat shock proteins in the adipose tissue |
| title_short | Immunohistochemical profiling of the heat shock response in obese non-diabetic subjects revealed impaired expression of heat shock proteins in the adipose tissue |
| title_sort | Immunohistochemical profiling of the heat shock response in obese non-diabetic subjects revealed impaired expression of heat shock proteins in the adipose tissue |
| topic | Biomedical and clinical sciences Medical biochemistry and metabolomics Health sciences Sports science and exercise Heat shock protein HSP Exercise ER stress Obesity Adipose tissue |