Computational and Transcriptome Analyses Revealed Preferential Induction of Chemotaxis and Lipid Synthesis by SARS-CoV-2
<p dir="ltr">The continuous and rapid emergence of new viral strains calls for a better understanding of the fundamental changes occurring within the host cell upon viral infection. In this study, we analyzed RNA-seq transcriptome data from Calu-3 human lung epithelial cells infected...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | |
| منشور في: |
2020
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| الموضوعات: | |
| الوسوم: |
إضافة وسم
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| _version_ | 1864513513508044800 |
|---|---|
| author | Hibah Shaath (5599658) |
| author2 | Nehad M. Alajez (7397276) |
| author2_role | author |
| author_facet | Hibah Shaath (5599658) Nehad M. Alajez (7397276) |
| author_role | author |
| dc.creator.none.fl_str_mv | Hibah Shaath (5599658) Nehad M. Alajez (7397276) |
| dc.date.none.fl_str_mv | 2020-09-01T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3390/biology9090260 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Computational_and_Transcriptome_Analyses_Revealed_Preferential_Induction_of_Chemotaxis_and_Lipid_Synthesis_by_SARS-CoV-2/25912096 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Genetics Microbiology Biomedical and clinical sciences Cardiovascular medicine and haematology Medical microbiology COVID-19 SARS-CoV-2 SARS-CoV SARS-MERS influenza A influenza B rhinovirus Bioinformatics gene expressions pathway analysis IFN response immune response fatty acid synthesis |
| dc.title.none.fl_str_mv | Computational and Transcriptome Analyses Revealed Preferential Induction of Chemotaxis and Lipid Synthesis by SARS-CoV-2 |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">The continuous and rapid emergence of new viral strains calls for a better understanding of the fundamental changes occurring within the host cell upon viral infection. In this study, we analyzed RNA-seq transcriptome data from Calu-3 human lung epithelial cells infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) compared to five other viruses namely, severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East Respiratory Syndrome (SARS-MERS), influenzavirus A (FLUA), influenzavirus B (FLUB), and rhinovirus (RHINO) compared to mock-infected cells and characterized their coding and noncoding RNA transcriptional portraits. The induction of interferon, inflammatory, and immune response was a hallmark of SARS-CoV-2 infection. Comprehensive bioinformatics revealed the activation of immune response and defense response to the virus as a common feature of viral infection. Interestingly however, the degree of functional categories and signaling pathways activation varied among different viruses. Ingenuity pathways analysis highlighted altered conical and casual pathways related to TNF, IL1A, and TLR7, which are seen more predominantly during SARS-CoV-2 infection. Nonetheless, the activation of chemotaxis and lipid synthesis was prominent in SARS-CoV-2-infected cells. Despite the commonality among all viruses, our data revealed the hyperactivation of chemotaxis and immune cell trafficking as well as the enhanced fatty acid synthesis as plausible mechanisms that could explain the inflammatory cytokine storms associated with severe cases of COVID-19 and the rapid spread of the virus, respectively.</p><h2>Other Information</h2><p dir="ltr">Published in: Biology<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/biology9090260" target="_blank">https://dx.doi.org/10.3390/biology9090260</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_5da275f33d867553ff686ef8fcbaa84a |
| identifier_str_mv | 10.3390/biology9090260 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25912096 |
| publishDate | 2020 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Computational and Transcriptome Analyses Revealed Preferential Induction of Chemotaxis and Lipid Synthesis by SARS-CoV-2Hibah Shaath (5599658)Nehad M. Alajez (7397276)Biological sciencesGeneticsMicrobiologyBiomedical and clinical sciencesCardiovascular medicine and haematologyMedical microbiologyCOVID-19SARS-CoV-2SARS-CoVSARS-MERSinfluenza Ainfluenza BrhinovirusBioinformaticsgene expressionspathway analysisIFN responseimmune responsefatty acid synthesis<p dir="ltr">The continuous and rapid emergence of new viral strains calls for a better understanding of the fundamental changes occurring within the host cell upon viral infection. In this study, we analyzed RNA-seq transcriptome data from Calu-3 human lung epithelial cells infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) compared to five other viruses namely, severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East Respiratory Syndrome (SARS-MERS), influenzavirus A (FLUA), influenzavirus B (FLUB), and rhinovirus (RHINO) compared to mock-infected cells and characterized their coding and noncoding RNA transcriptional portraits. The induction of interferon, inflammatory, and immune response was a hallmark of SARS-CoV-2 infection. Comprehensive bioinformatics revealed the activation of immune response and defense response to the virus as a common feature of viral infection. Interestingly however, the degree of functional categories and signaling pathways activation varied among different viruses. Ingenuity pathways analysis highlighted altered conical and casual pathways related to TNF, IL1A, and TLR7, which are seen more predominantly during SARS-CoV-2 infection. Nonetheless, the activation of chemotaxis and lipid synthesis was prominent in SARS-CoV-2-infected cells. Despite the commonality among all viruses, our data revealed the hyperactivation of chemotaxis and immune cell trafficking as well as the enhanced fatty acid synthesis as plausible mechanisms that could explain the inflammatory cytokine storms associated with severe cases of COVID-19 and the rapid spread of the virus, respectively.</p><h2>Other Information</h2><p dir="ltr">Published in: Biology<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/biology9090260" target="_blank">https://dx.doi.org/10.3390/biology9090260</a></p>2020-09-01T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3390/biology9090260https://figshare.com/articles/journal_contribution/Computational_and_Transcriptome_Analyses_Revealed_Preferential_Induction_of_Chemotaxis_and_Lipid_Synthesis_by_SARS-CoV-2/25912096CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/259120962020-09-01T03:00:00Z |
| spellingShingle | Computational and Transcriptome Analyses Revealed Preferential Induction of Chemotaxis and Lipid Synthesis by SARS-CoV-2 Hibah Shaath (5599658) Biological sciences Genetics Microbiology Biomedical and clinical sciences Cardiovascular medicine and haematology Medical microbiology COVID-19 SARS-CoV-2 SARS-CoV SARS-MERS influenza A influenza B rhinovirus Bioinformatics gene expressions pathway analysis IFN response immune response fatty acid synthesis |
| status_str | publishedVersion |
| title | Computational and Transcriptome Analyses Revealed Preferential Induction of Chemotaxis and Lipid Synthesis by SARS-CoV-2 |
| title_full | Computational and Transcriptome Analyses Revealed Preferential Induction of Chemotaxis and Lipid Synthesis by SARS-CoV-2 |
| title_fullStr | Computational and Transcriptome Analyses Revealed Preferential Induction of Chemotaxis and Lipid Synthesis by SARS-CoV-2 |
| title_full_unstemmed | Computational and Transcriptome Analyses Revealed Preferential Induction of Chemotaxis and Lipid Synthesis by SARS-CoV-2 |
| title_short | Computational and Transcriptome Analyses Revealed Preferential Induction of Chemotaxis and Lipid Synthesis by SARS-CoV-2 |
| title_sort | Computational and Transcriptome Analyses Revealed Preferential Induction of Chemotaxis and Lipid Synthesis by SARS-CoV-2 |
| topic | Biological sciences Genetics Microbiology Biomedical and clinical sciences Cardiovascular medicine and haematology Medical microbiology COVID-19 SARS-CoV-2 SARS-CoV SARS-MERS influenza A influenza B rhinovirus Bioinformatics gene expressions pathway analysis IFN response immune response fatty acid synthesis |