Uhrf1 regulates adipogenesis through GPNMB mediated TGFBeta axis
<p><strong>Poster by Muneera Vakayil (Weill Cornell Medicine - Qatar, Hamad Bin Khalifa University), Aisha Madani (Weill Cornell Medicine - Qatar), Yasser Majeed (Weill Cornell Medicine - Qatar), and Nayef Mazloum (Weill Cornell Medicine - Qatar)</strong></p> <p>Backgro...
محفوظ في:
| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | , , |
| منشور في: |
2023
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| الموضوعات: | |
| الوسوم: |
إضافة وسم
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| _version_ | 1864513564677505024 |
|---|---|
| author | Muneera Vakayil (12037693) |
| author2 | Aisha Madani (15430957) Yasser Majeed (8779407) Nayef Mazloum (1275666) |
| author2_role | author author author |
| author_facet | Muneera Vakayil (12037693) Aisha Madani (15430957) Yasser Majeed (8779407) Nayef Mazloum (1275666) |
| author_role | author |
| dc.creator.none.fl_str_mv | Muneera Vakayil (12037693) Aisha Madani (15430957) Yasser Majeed (8779407) Nayef Mazloum (1275666) |
| dc.date.none.fl_str_mv | 2023-05-17T12:01:58Z |
| dc.identifier.none.fl_str_mv | 10.57945/manara.22785659.v1 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/poster/Uhrf1_regulates_adipogenesis_through_GPNMB_mediated_TGFBeta_axis/22785659 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Medical biotechnology Uhrf1 Adipogenesis Obesity Inflammation Type 2 Diabetes Mellitus |
| dc.title.none.fl_str_mv | Uhrf1 regulates adipogenesis through GPNMB mediated TGFBeta axis |
| dc.type.none.fl_str_mv | Image Poster info:eu-repo/semantics/publishedVersion image |
| description | <p><strong>Poster by Muneera Vakayil (Weill Cornell Medicine - Qatar, Hamad Bin Khalifa University), Aisha Madani (Weill Cornell Medicine - Qatar), Yasser Majeed (Weill Cornell Medicine - Qatar), and Nayef Mazloum (Weill Cornell Medicine - Qatar)</strong></p> <p>Background: The increased prevalence of obesity has led to a concomitant increase in the incidence of type 2 diabetes (T2D). Obesity-linked inflammation is the major cause of insulin resistance, contributing to the onset of T2D. Therefore, understanding the cellular mechanisms responsible for adipogenesis, the conversion of preadipocytes to adipocytes, is critical for identifying anti-diabetic therapeutic targets. Uhrf1 is a key epigenetic regulator that coordinates DNA methylation and histone modifications; however, the function of Uhrf1 in metabolic diseases is not known. </p> <p>Objective: Therefore, here, we aimed to understand the role of Uhrf1 in adipogenesis by studying Uhrf1 loss of function cells.</p> <p>Methods: We performed CRISPR/ Cas9 and shRNA-mediated gene silencing in addition to whole transcriptomics analysis on Uhrf1 KO cells.</p> <p>Results: We identified altered molecular and cellular pathways indicating that Uhrf1 is a critical factor required for adipocyte metabolism, and in its absence, cells exhibit adipocyte dysfunctions with higher levels of inflammatory markers.</p> <p>Conclusion: Our results highlight the possibility of Uhrf1 and its targets to have clinical applications in obesity and T2D treatment.</p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_5ea9d0cdb609cd7e8e66f4a7ce1c9c13 |
| identifier_str_mv | 10.57945/manara.22785659.v1 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/22785659 |
| publishDate | 2023 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Uhrf1 regulates adipogenesis through GPNMB mediated TGFBeta axisMuneera Vakayil (12037693)Aisha Madani (15430957)Yasser Majeed (8779407)Nayef Mazloum (1275666)Biomedical and clinical sciencesMedical biotechnologyUhrf1AdipogenesisObesityInflammationType 2 Diabetes Mellitus<p><strong>Poster by Muneera Vakayil (Weill Cornell Medicine - Qatar, Hamad Bin Khalifa University), Aisha Madani (Weill Cornell Medicine - Qatar), Yasser Majeed (Weill Cornell Medicine - Qatar), and Nayef Mazloum (Weill Cornell Medicine - Qatar)</strong></p> <p>Background: The increased prevalence of obesity has led to a concomitant increase in the incidence of type 2 diabetes (T2D). Obesity-linked inflammation is the major cause of insulin resistance, contributing to the onset of T2D. Therefore, understanding the cellular mechanisms responsible for adipogenesis, the conversion of preadipocytes to adipocytes, is critical for identifying anti-diabetic therapeutic targets. Uhrf1 is a key epigenetic regulator that coordinates DNA methylation and histone modifications; however, the function of Uhrf1 in metabolic diseases is not known. </p> <p>Objective: Therefore, here, we aimed to understand the role of Uhrf1 in adipogenesis by studying Uhrf1 loss of function cells.</p> <p>Methods: We performed CRISPR/ Cas9 and shRNA-mediated gene silencing in addition to whole transcriptomics analysis on Uhrf1 KO cells.</p> <p>Results: We identified altered molecular and cellular pathways indicating that Uhrf1 is a critical factor required for adipocyte metabolism, and in its absence, cells exhibit adipocyte dysfunctions with higher levels of inflammatory markers.</p> <p>Conclusion: Our results highlight the possibility of Uhrf1 and its targets to have clinical applications in obesity and T2D treatment.</p>2023-05-17T12:01:58ZImagePosterinfo:eu-repo/semantics/publishedVersionimage10.57945/manara.22785659.v1https://figshare.com/articles/poster/Uhrf1_regulates_adipogenesis_through_GPNMB_mediated_TGFBeta_axis/22785659CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/227856592023-05-17T12:01:58Z |
| spellingShingle | Uhrf1 regulates adipogenesis through GPNMB mediated TGFBeta axis Muneera Vakayil (12037693) Biomedical and clinical sciences Medical biotechnology Uhrf1 Adipogenesis Obesity Inflammation Type 2 Diabetes Mellitus |
| status_str | publishedVersion |
| title | Uhrf1 regulates adipogenesis through GPNMB mediated TGFBeta axis |
| title_full | Uhrf1 regulates adipogenesis through GPNMB mediated TGFBeta axis |
| title_fullStr | Uhrf1 regulates adipogenesis through GPNMB mediated TGFBeta axis |
| title_full_unstemmed | Uhrf1 regulates adipogenesis through GPNMB mediated TGFBeta axis |
| title_short | Uhrf1 regulates adipogenesis through GPNMB mediated TGFBeta axis |
| title_sort | Uhrf1 regulates adipogenesis through GPNMB mediated TGFBeta axis |
| topic | Biomedical and clinical sciences Medical biotechnology Uhrf1 Adipogenesis Obesity Inflammation Type 2 Diabetes Mellitus |