In vitro Anti-Helicobacter pylori Activity of Capsaicin
<p dir="ltr">Worldwide, peptic ulcer and gastritis considered to be one of the biggest health challenge, <i>Helicobacter pylori</i> is responsible for more than eighty percent of chronic active gastritis where continual infection remains for decennary. However, the succes...
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| مؤلفون آخرون: | , , , , , , , |
| منشور في: |
2020
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إضافة وسم
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| _version_ | 1864513505264140288 |
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| author | Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Jordan, Amman, Jordan. (19672696) |
| author2 | Isra Tayseer (19672699) Talal Aburjai (19672702) Luay Abu-Qatouseh (12106047) Faculty of Pharmacy, Department of Biomedical Sciences, University of Petra, Amman, Jordan. (19672708) Nehaya AL-Karabieh (19672711) Department of Plant Protection, School of Agriculture, The University of Jordan, Amman, Jordan. (19672714) Wesam Ahmed (4870942) Ali Al-Samydai (18176017) |
| author2_role | author author author author author author author author |
| author_facet | Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Jordan, Amman, Jordan. (19672696) Isra Tayseer (19672699) Talal Aburjai (19672702) Luay Abu-Qatouseh (12106047) Faculty of Pharmacy, Department of Biomedical Sciences, University of Petra, Amman, Jordan. (19672708) Nehaya AL-Karabieh (19672711) Department of Plant Protection, School of Agriculture, The University of Jordan, Amman, Jordan. (19672714) Wesam Ahmed (4870942) Ali Al-Samydai (18176017) |
| author_role | author |
| dc.creator.none.fl_str_mv | Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Jordan, Amman, Jordan. (19672696) Isra Tayseer (19672699) Talal Aburjai (19672702) Luay Abu-Qatouseh (12106047) Faculty of Pharmacy, Department of Biomedical Sciences, University of Petra, Amman, Jordan. (19672708) Nehaya AL-Karabieh (19672711) Department of Plant Protection, School of Agriculture, The University of Jordan, Amman, Jordan. (19672714) Wesam Ahmed (4870942) Ali Al-Samydai (18176017) |
| dc.date.none.fl_str_mv | 2020-03-21T06:00:00Z |
| dc.identifier.none.fl_str_mv | 10.22207/jpam.14.1.29 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/In_vitro_Anti-Helicobacter_pylori_Activity_of_Capsaicin/27021688 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Microbiology Biomedical and clinical sciences Pharmacology and pharmaceutical sciences H. pylori Capsaicin Spices Synergism |
| dc.title.none.fl_str_mv | In vitro Anti-Helicobacter pylori Activity of Capsaicin |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Worldwide, peptic ulcer and gastritis considered to be one of the biggest health challenge, <i>Helicobacter pylori</i> is responsible for more than eighty percent of chronic active gastritis where continual infection remains for decennary. However, the success of commercially available drugs for the management of <i>H. pylori</i> has overwhelmed by antibiotic-resistant strains, especially, metronidazole and clarithromycin, therefore, an urgent need arise to search for new options for treatment with enhanced anti- <i>H. pylori</i> activities, while being less toxic to human cells. Naturally occurring plant products, including spices, are one of these strategies that showed activity against <i>H. pylori</i>. Present study aim to test the antibacterial activity of capsaicin and other pure plant-derived compounds against a standard (NCTC 11916) H. pylori strain In vitro and to test for possible synergistic effect when combined with conventional therapy. Capsaicin shows good antibacterial activity on regular antimicrobial sensitivity testing methods (Anti-MSTM) and titration checkerboard assay MIC (0.0625 mg/ml), whereas piperine MIC was (0.125 mg/ml). While for curcumin no inhibition was found. The strain was found to be resistant to metronidazole with (MIC=250 μg/ml). When combining capsaicin with metronidazole, (FIC) Fractional inhibitory concentration values shown a synergistic effect, While the additive effect was found for capsaicin combination with piperine. Our obtained data indicate that capsaicin possesses promising anti <i>H. pylori</i> bioactivity and synergistic activity when combined with metronidazole but more work is necessary to examine the mechanisms by which these happened. Furthermore, it is necessary to ensure its activity against <i>H. pylori</i> <i>In vivo</i> and clinical settings.</p><h2>Other Information</h2><p dir="ltr">Published in: Journal of Pure and Applied Microbiology<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.22207/jpam.14.1.29" target="_blank">https://dx.doi.org/10.22207/jpam.14.1.29</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_66269cd272ba1534498ef0af51f1dab3 |
| identifier_str_mv | 10.22207/jpam.14.1.29 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/27021688 |
| publishDate | 2020 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | In vitro Anti-Helicobacter pylori Activity of CapsaicinDepartment of Pharmaceutical Sciences, Faculty of Pharmacy, University of Jordan, Amman, Jordan. (19672696)Isra Tayseer (19672699)Talal Aburjai (19672702)Luay Abu-Qatouseh (12106047)Faculty of Pharmacy, Department of Biomedical Sciences, University of Petra, Amman, Jordan. (19672708)Nehaya AL-Karabieh (19672711)Department of Plant Protection, School of Agriculture, The University of Jordan, Amman, Jordan. (19672714)Wesam Ahmed (4870942)Ali Al-Samydai (18176017)Biological sciencesMicrobiologyBiomedical and clinical sciencesPharmacology and pharmaceutical sciencesH. pyloriCapsaicinSpicesSynergism<p dir="ltr">Worldwide, peptic ulcer and gastritis considered to be one of the biggest health challenge, <i>Helicobacter pylori</i> is responsible for more than eighty percent of chronic active gastritis where continual infection remains for decennary. However, the success of commercially available drugs for the management of <i>H. pylori</i> has overwhelmed by antibiotic-resistant strains, especially, metronidazole and clarithromycin, therefore, an urgent need arise to search for new options for treatment with enhanced anti- <i>H. pylori</i> activities, while being less toxic to human cells. Naturally occurring plant products, including spices, are one of these strategies that showed activity against <i>H. pylori</i>. Present study aim to test the antibacterial activity of capsaicin and other pure plant-derived compounds against a standard (NCTC 11916) H. pylori strain In vitro and to test for possible synergistic effect when combined with conventional therapy. Capsaicin shows good antibacterial activity on regular antimicrobial sensitivity testing methods (Anti-MSTM) and titration checkerboard assay MIC (0.0625 mg/ml), whereas piperine MIC was (0.125 mg/ml). While for curcumin no inhibition was found. The strain was found to be resistant to metronidazole with (MIC=250 μg/ml). When combining capsaicin with metronidazole, (FIC) Fractional inhibitory concentration values shown a synergistic effect, While the additive effect was found for capsaicin combination with piperine. Our obtained data indicate that capsaicin possesses promising anti <i>H. pylori</i> bioactivity and synergistic activity when combined with metronidazole but more work is necessary to examine the mechanisms by which these happened. Furthermore, it is necessary to ensure its activity against <i>H. pylori</i> <i>In vivo</i> and clinical settings.</p><h2>Other Information</h2><p dir="ltr">Published in: Journal of Pure and Applied Microbiology<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.22207/jpam.14.1.29" target="_blank">https://dx.doi.org/10.22207/jpam.14.1.29</a></p>2020-03-21T06:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.22207/jpam.14.1.29https://figshare.com/articles/journal_contribution/In_vitro_Anti-Helicobacter_pylori_Activity_of_Capsaicin/27021688CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/270216882020-03-21T06:00:00Z |
| spellingShingle | In vitro Anti-Helicobacter pylori Activity of Capsaicin Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Jordan, Amman, Jordan. (19672696) Biological sciences Microbiology Biomedical and clinical sciences Pharmacology and pharmaceutical sciences H. pylori Capsaicin Spices Synergism |
| status_str | publishedVersion |
| title | In vitro Anti-Helicobacter pylori Activity of Capsaicin |
| title_full | In vitro Anti-Helicobacter pylori Activity of Capsaicin |
| title_fullStr | In vitro Anti-Helicobacter pylori Activity of Capsaicin |
| title_full_unstemmed | In vitro Anti-Helicobacter pylori Activity of Capsaicin |
| title_short | In vitro Anti-Helicobacter pylori Activity of Capsaicin |
| title_sort | In vitro Anti-Helicobacter pylori Activity of Capsaicin |
| topic | Biological sciences Microbiology Biomedical and clinical sciences Pharmacology and pharmaceutical sciences H. pylori Capsaicin Spices Synergism |