Distinct antibody repertoires against endemic human coronaviruses in children and adults
<p dir="ltr">Four endemic human coronaviruses (HCoVs) are commonly associated with acute respiratory infection in humans. B cell responses to these “common cold” viruses remain incompletely understood. Here we report a comprehensive analysis of CoV-specific antibody repertoires in 23...
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2021
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| _version_ | 1864513516813156352 |
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| author | Taushif Khan (691254) |
| author2 | Mahbuba Rahman (198499) Fatima Al Ali (13210926) Susie S.Y. Huang (18282292) Manar Ata (9046473) Qian Zhang (49333) Paul Bastard (12967485) Zhiyong Liu (160339) Emmanuelle Jouanguy (64694) Vivien Beziat (18508176) Aurélie Cobat (2160433) Gheyath K. Nasrallah (9200525) Hadi M. Yassine (4675846) Maria K. Smatti (4675852) Amira Saeed (14152857) Isabelle Vandernoot (18508179) Jean-Christophe Goffard (5913116) Guillaume Smits (4260148) Isabelle Migeotte (245039) Filomeen Haerynck (5913119) Isabelle Meyts (8475399) Laurent Abel (69833) Jean-Laurent Casanova (64712) Mohammad R. Hasan (13777597) Nico Marr (349853) |
| author2_role | author author author author author author author author author author author author author author author author author author author author author author author author |
| author_facet | Taushif Khan (691254) Mahbuba Rahman (198499) Fatima Al Ali (13210926) Susie S.Y. Huang (18282292) Manar Ata (9046473) Qian Zhang (49333) Paul Bastard (12967485) Zhiyong Liu (160339) Emmanuelle Jouanguy (64694) Vivien Beziat (18508176) Aurélie Cobat (2160433) Gheyath K. Nasrallah (9200525) Hadi M. Yassine (4675846) Maria K. Smatti (4675852) Amira Saeed (14152857) Isabelle Vandernoot (18508179) Jean-Christophe Goffard (5913116) Guillaume Smits (4260148) Isabelle Migeotte (245039) Filomeen Haerynck (5913119) Isabelle Meyts (8475399) Laurent Abel (69833) Jean-Laurent Casanova (64712) Mohammad R. Hasan (13777597) Nico Marr (349853) |
| author_role | author |
| dc.creator.none.fl_str_mv | Taushif Khan (691254) Mahbuba Rahman (198499) Fatima Al Ali (13210926) Susie S.Y. Huang (18282292) Manar Ata (9046473) Qian Zhang (49333) Paul Bastard (12967485) Zhiyong Liu (160339) Emmanuelle Jouanguy (64694) Vivien Beziat (18508176) Aurélie Cobat (2160433) Gheyath K. Nasrallah (9200525) Hadi M. Yassine (4675846) Maria K. Smatti (4675852) Amira Saeed (14152857) Isabelle Vandernoot (18508179) Jean-Christophe Goffard (5913116) Guillaume Smits (4260148) Isabelle Migeotte (245039) Filomeen Haerynck (5913119) Isabelle Meyts (8475399) Laurent Abel (69833) Jean-Laurent Casanova (64712) Mohammad R. Hasan (13777597) Nico Marr (349853) |
| dc.date.none.fl_str_mv | 2021-01-26T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1172/jci.insight.144499 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Distinct_antibody_repertoires_against_endemic_human_coronaviruses_in_children_and_adults/25756260 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Clinical sciences Immunology Endemic human coronaviruses (HCoVs) Acute respiratory infection B cell responses Common cold viruses Antibody repertoires Phage immunoprecipitation sequencing Seroprevalence Linear B cell epitopes |
| dc.title.none.fl_str_mv | Distinct antibody repertoires against endemic human coronaviruses in children and adults |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Four endemic human coronaviruses (HCoVs) are commonly associated with acute respiratory infection in humans. B cell responses to these “common cold” viruses remain incompletely understood. Here we report a comprehensive analysis of CoV-specific antibody repertoires in 231 children and 1168 adults using phage immunoprecipitation sequencing. Seroprevalence of antibodies against endemic HCoVs ranged between approximately 4% and 27% depending on the species and cohort. We identified at least 136 novel linear B cell epitopes. Antibody repertoires against endemic HCoVs were qualitatively different between children and adults in that anti-HCoV IgG specificities more frequently found among children targeted functionally important and structurally conserved regions of the spike, nucleocapsid, and matrix proteins. Moreover, antibody specificities targeting the highly conserved fusion peptide region and S2′ cleavage site of the spike protein were broadly cross-reactive with peptides of epidemic human and nonhuman coronaviruses. In contrast, an acidic tandem repeat in the N-terminal region of the Nsp3 subdomain of the HCoV-HKU1 polyprotein was the predominant target of antibody responses in adult donors. Our findings shed light on the dominant species-specific and pan-CoV target sites of human antibody responses to coronavirus infection, thereby providing important insights for the development of prophylactic or therapeutic monoclonal antibodies and vaccine design.</p><h2>Other Information</h2><p dir="ltr">Published in: JCI Insight<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1172/jci.insight.144499" target="_blank">https://dx.doi.org/10.1172/jci.insight.144499</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_68d66abccf53cb574355f18a57a75d62 |
| identifier_str_mv | 10.1172/jci.insight.144499 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25756260 |
| publishDate | 2021 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Distinct antibody repertoires against endemic human coronaviruses in children and adultsTaushif Khan (691254)Mahbuba Rahman (198499)Fatima Al Ali (13210926)Susie S.Y. Huang (18282292)Manar Ata (9046473)Qian Zhang (49333)Paul Bastard (12967485)Zhiyong Liu (160339)Emmanuelle Jouanguy (64694)Vivien Beziat (18508176)Aurélie Cobat (2160433)Gheyath K. Nasrallah (9200525)Hadi M. Yassine (4675846)Maria K. Smatti (4675852)Amira Saeed (14152857)Isabelle Vandernoot (18508179)Jean-Christophe Goffard (5913116)Guillaume Smits (4260148)Isabelle Migeotte (245039)Filomeen Haerynck (5913119)Isabelle Meyts (8475399)Laurent Abel (69833)Jean-Laurent Casanova (64712)Mohammad R. Hasan (13777597)Nico Marr (349853)Biomedical and clinical sciencesClinical sciencesImmunologyEndemic human coronaviruses (HCoVs)Acute respiratory infectionB cell responsesCommon cold virusesAntibody repertoiresPhage immunoprecipitation sequencingSeroprevalence LinearB cell epitopes<p dir="ltr">Four endemic human coronaviruses (HCoVs) are commonly associated with acute respiratory infection in humans. B cell responses to these “common cold” viruses remain incompletely understood. Here we report a comprehensive analysis of CoV-specific antibody repertoires in 231 children and 1168 adults using phage immunoprecipitation sequencing. Seroprevalence of antibodies against endemic HCoVs ranged between approximately 4% and 27% depending on the species and cohort. We identified at least 136 novel linear B cell epitopes. Antibody repertoires against endemic HCoVs were qualitatively different between children and adults in that anti-HCoV IgG specificities more frequently found among children targeted functionally important and structurally conserved regions of the spike, nucleocapsid, and matrix proteins. Moreover, antibody specificities targeting the highly conserved fusion peptide region and S2′ cleavage site of the spike protein were broadly cross-reactive with peptides of epidemic human and nonhuman coronaviruses. In contrast, an acidic tandem repeat in the N-terminal region of the Nsp3 subdomain of the HCoV-HKU1 polyprotein was the predominant target of antibody responses in adult donors. Our findings shed light on the dominant species-specific and pan-CoV target sites of human antibody responses to coronavirus infection, thereby providing important insights for the development of prophylactic or therapeutic monoclonal antibodies and vaccine design.</p><h2>Other Information</h2><p dir="ltr">Published in: JCI Insight<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1172/jci.insight.144499" target="_blank">https://dx.doi.org/10.1172/jci.insight.144499</a></p>2021-01-26T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1172/jci.insight.144499https://figshare.com/articles/journal_contribution/Distinct_antibody_repertoires_against_endemic_human_coronaviruses_in_children_and_adults/25756260CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/257562602021-01-26T03:00:00Z |
| spellingShingle | Distinct antibody repertoires against endemic human coronaviruses in children and adults Taushif Khan (691254) Biomedical and clinical sciences Clinical sciences Immunology Endemic human coronaviruses (HCoVs) Acute respiratory infection B cell responses Common cold viruses Antibody repertoires Phage immunoprecipitation sequencing Seroprevalence Linear B cell epitopes |
| status_str | publishedVersion |
| title | Distinct antibody repertoires against endemic human coronaviruses in children and adults |
| title_full | Distinct antibody repertoires against endemic human coronaviruses in children and adults |
| title_fullStr | Distinct antibody repertoires against endemic human coronaviruses in children and adults |
| title_full_unstemmed | Distinct antibody repertoires against endemic human coronaviruses in children and adults |
| title_short | Distinct antibody repertoires against endemic human coronaviruses in children and adults |
| title_sort | Distinct antibody repertoires against endemic human coronaviruses in children and adults |
| topic | Biomedical and clinical sciences Clinical sciences Immunology Endemic human coronaviruses (HCoVs) Acute respiratory infection B cell responses Common cold viruses Antibody repertoires Phage immunoprecipitation sequencing Seroprevalence Linear B cell epitopes |