Analysis of Serum Advanced Glycation Endproducts Reveals Methylglyoxal-Derived Advanced Glycation MG-H1 Free Adduct Is a Risk Marker in Non-Diabetic and Diabetic Chronic Kidney Disease

<p dir="ltr">Accumulation of advanced glycation endproducts (AGEs) is linked to decline in renal function, particularly in patients with diabetes. Major forms of AGEs in serum are protein-bound AGEs and AGE free adducts. In this study, we assessed levels of AGEs in subjects with and...

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محفوظ في:
التفاصيل البيبلوغرافية
المؤلف الرئيسي: Naila Rabbani (291722) (author)
مؤلفون آخرون: Antonysunil Adaikalakoteswari (325054) (author), James R. Larkin (12221192) (author), Sianna Panagiotopoulos (3505712) (author), Richard J. MacIsaac (9718733) (author), Dennis K. Yue (19482394) (author), Gregory R. Fulcher (7774967) (author), Matthew A. Roberts (3056739) (author), Merlin Thomas (6252059) (author), Elif Ekinci (3913966) (author), Paul J. Thornalley (291723) (author)
منشور في: 2022
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author Naila Rabbani (291722)
author2 Antonysunil Adaikalakoteswari (325054)
James R. Larkin (12221192)
Sianna Panagiotopoulos (3505712)
Richard J. MacIsaac (9718733)
Dennis K. Yue (19482394)
Gregory R. Fulcher (7774967)
Matthew A. Roberts (3056739)
Merlin Thomas (6252059)
Elif Ekinci (3913966)
Paul J. Thornalley (291723)
author2_role author
author
author
author
author
author
author
author
author
author
author_facet Naila Rabbani (291722)
Antonysunil Adaikalakoteswari (325054)
James R. Larkin (12221192)
Sianna Panagiotopoulos (3505712)
Richard J. MacIsaac (9718733)
Dennis K. Yue (19482394)
Gregory R. Fulcher (7774967)
Matthew A. Roberts (3056739)
Merlin Thomas (6252059)
Elif Ekinci (3913966)
Paul J. Thornalley (291723)
author_role author
dc.creator.none.fl_str_mv Naila Rabbani (291722)
Antonysunil Adaikalakoteswari (325054)
James R. Larkin (12221192)
Sianna Panagiotopoulos (3505712)
Richard J. MacIsaac (9718733)
Dennis K. Yue (19482394)
Gregory R. Fulcher (7774967)
Matthew A. Roberts (3056739)
Merlin Thomas (6252059)
Elif Ekinci (3913966)
Paul J. Thornalley (291723)
dc.date.none.fl_str_mv 2022-12-21T06:00:00Z
dc.identifier.none.fl_str_mv 10.3390/ijms24010152
dc.relation.none.fl_str_mv https://figshare.com/articles/journal_contribution/Analysis_of_Serum_Advanced_Glycation_Endproducts_Reveals_Methylglyoxal-Derived_Advanced_Glycation_MG-H1_Free_Adduct_Is_a_Risk_Marker_in_Non-Diabetic_and_Diabetic_Chronic_Kidney_Disease/26830183
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Biomedical and clinical sciences
Clinical sciences
Medical biochemistry and metabolomics
chronic kidney disease
diabetes
glycation
methylglyoxal
estimated glomerular filtration rate
dc.title.none.fl_str_mv Analysis of Serum Advanced Glycation Endproducts Reveals Methylglyoxal-Derived Advanced Glycation MG-H1 Free Adduct Is a Risk Marker in Non-Diabetic and Diabetic Chronic Kidney Disease
dc.type.none.fl_str_mv Text
Journal contribution
info:eu-repo/semantics/publishedVersion
text
contribution to journal
description <p dir="ltr">Accumulation of advanced glycation endproducts (AGEs) is linked to decline in renal function, particularly in patients with diabetes. Major forms of AGEs in serum are protein-bound AGEs and AGE free adducts. In this study, we assessed levels of AGEs in subjects with and without diabetes, with normal renal function and stages 2 to 4 chronic kidney disease (CKD), to identify which AGE has the greatest progressive change with decline in renal function and change in diabetes. We performed a cross-sectional study of patients with stages 2–4 CKD, with and without diabetes, and healthy controls (<i>n </i>= 135). Nine protein-bound and free adduct AGEs were quantified in serum. Most protein-bound AGEs increased moderately through stages 2–4 CKD whereas AGE free adducts increased markedly. Methylglyoxal-derived hydroimidazolone MG-H1 free adduct was the AGE most responsive to CKD status, increasing 8-fold and 30-fold in stage 4 CKD in patients without and with diabetes, respectively. MG-H1 Glomerular filtration flux was increased 5-fold in diabetes, likely reflecting increased methylglyoxal glycation status. We conclude that serum MG-H1 free adduct concentration was strongly related to stage of CKD and increased in diabetes status. Serum MG-H1 free adduct is a candidate AGE risk marker of non-diabetic and diabetic CKD.</p><h2>Other Information</h2><p dir="ltr">Published in: International Journal of Molecular Sciences<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/ijms24010152" target="_blank">https://dx.doi.org/10.3390/ijms24010152</a></p>
eu_rights_str_mv openAccess
id Manara2_68d7950ce8a904eca881046ad4e003e0
identifier_str_mv 10.3390/ijms24010152
network_acronym_str Manara2
network_name_str Manara2
oai_identifier_str oai:figshare.com:article/26830183
publishDate 2022
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spelling Analysis of Serum Advanced Glycation Endproducts Reveals Methylglyoxal-Derived Advanced Glycation MG-H1 Free Adduct Is a Risk Marker in Non-Diabetic and Diabetic Chronic Kidney DiseaseNaila Rabbani (291722)Antonysunil Adaikalakoteswari (325054)James R. Larkin (12221192)Sianna Panagiotopoulos (3505712)Richard J. MacIsaac (9718733)Dennis K. Yue (19482394)Gregory R. Fulcher (7774967)Matthew A. Roberts (3056739)Merlin Thomas (6252059)Elif Ekinci (3913966)Paul J. Thornalley (291723)Biomedical and clinical sciencesClinical sciencesMedical biochemistry and metabolomicschronic kidney diseasediabetesglycationmethylglyoxalestimated glomerular filtration rate<p dir="ltr">Accumulation of advanced glycation endproducts (AGEs) is linked to decline in renal function, particularly in patients with diabetes. Major forms of AGEs in serum are protein-bound AGEs and AGE free adducts. In this study, we assessed levels of AGEs in subjects with and without diabetes, with normal renal function and stages 2 to 4 chronic kidney disease (CKD), to identify which AGE has the greatest progressive change with decline in renal function and change in diabetes. We performed a cross-sectional study of patients with stages 2–4 CKD, with and without diabetes, and healthy controls (<i>n </i>= 135). Nine protein-bound and free adduct AGEs were quantified in serum. Most protein-bound AGEs increased moderately through stages 2–4 CKD whereas AGE free adducts increased markedly. Methylglyoxal-derived hydroimidazolone MG-H1 free adduct was the AGE most responsive to CKD status, increasing 8-fold and 30-fold in stage 4 CKD in patients without and with diabetes, respectively. MG-H1 Glomerular filtration flux was increased 5-fold in diabetes, likely reflecting increased methylglyoxal glycation status. We conclude that serum MG-H1 free adduct concentration was strongly related to stage of CKD and increased in diabetes status. Serum MG-H1 free adduct is a candidate AGE risk marker of non-diabetic and diabetic CKD.</p><h2>Other Information</h2><p dir="ltr">Published in: International Journal of Molecular Sciences<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/ijms24010152" target="_blank">https://dx.doi.org/10.3390/ijms24010152</a></p>2022-12-21T06:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3390/ijms24010152https://figshare.com/articles/journal_contribution/Analysis_of_Serum_Advanced_Glycation_Endproducts_Reveals_Methylglyoxal-Derived_Advanced_Glycation_MG-H1_Free_Adduct_Is_a_Risk_Marker_in_Non-Diabetic_and_Diabetic_Chronic_Kidney_Disease/26830183CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/268301832022-12-21T06:00:00Z
spellingShingle Analysis of Serum Advanced Glycation Endproducts Reveals Methylglyoxal-Derived Advanced Glycation MG-H1 Free Adduct Is a Risk Marker in Non-Diabetic and Diabetic Chronic Kidney Disease
Naila Rabbani (291722)
Biomedical and clinical sciences
Clinical sciences
Medical biochemistry and metabolomics
chronic kidney disease
diabetes
glycation
methylglyoxal
estimated glomerular filtration rate
status_str publishedVersion
title Analysis of Serum Advanced Glycation Endproducts Reveals Methylglyoxal-Derived Advanced Glycation MG-H1 Free Adduct Is a Risk Marker in Non-Diabetic and Diabetic Chronic Kidney Disease
title_full Analysis of Serum Advanced Glycation Endproducts Reveals Methylglyoxal-Derived Advanced Glycation MG-H1 Free Adduct Is a Risk Marker in Non-Diabetic and Diabetic Chronic Kidney Disease
title_fullStr Analysis of Serum Advanced Glycation Endproducts Reveals Methylglyoxal-Derived Advanced Glycation MG-H1 Free Adduct Is a Risk Marker in Non-Diabetic and Diabetic Chronic Kidney Disease
title_full_unstemmed Analysis of Serum Advanced Glycation Endproducts Reveals Methylglyoxal-Derived Advanced Glycation MG-H1 Free Adduct Is a Risk Marker in Non-Diabetic and Diabetic Chronic Kidney Disease
title_short Analysis of Serum Advanced Glycation Endproducts Reveals Methylglyoxal-Derived Advanced Glycation MG-H1 Free Adduct Is a Risk Marker in Non-Diabetic and Diabetic Chronic Kidney Disease
title_sort Analysis of Serum Advanced Glycation Endproducts Reveals Methylglyoxal-Derived Advanced Glycation MG-H1 Free Adduct Is a Risk Marker in Non-Diabetic and Diabetic Chronic Kidney Disease
topic Biomedical and clinical sciences
Clinical sciences
Medical biochemistry and metabolomics
chronic kidney disease
diabetes
glycation
methylglyoxal
estimated glomerular filtration rate