Synthetic Cannabinoid AB-FUBINACA Negatively Impacted the Male Fertility and Induced Testicular Toxicity

<h3 dir="ltr">Purpose</h3><p dir="ltr">The recreational use of synthetic cannabinoids (SCs) by adolescents and adults has markedly increased in recent years. Previous studies demonstrated that exposure to SCs is associated with multiple adverse health effects. N...

وصف كامل

محفوظ في:
التفاصيل البيبلوغرافية
المؤلف الرئيسي: Ayman Alzu’bi (13162392) (author)
مؤلفون آخرون: Ejlal Abu-El-Rub (5168183) (author), Fatimah A. Almahasneh (22224547) (author), Rawan Almazari (21842705) (author), Amani Kasasbeh (22330402) (author), Heba F. AI-jariri (22224550) (author), Amneh Alrabie (23073376) (author), Raed M. Al-Zoubi (2037490) (author)
منشور في: 2025
الموضوعات:
الوسوم: إضافة وسم
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author Ayman Alzu’bi (13162392)
author2 Ejlal Abu-El-Rub (5168183)
Fatimah A. Almahasneh (22224547)
Rawan Almazari (21842705)
Amani Kasasbeh (22330402)
Heba F. AI-jariri (22224550)
Amneh Alrabie (23073376)
Raed M. Al-Zoubi (2037490)
author2_role author
author
author
author
author
author
author
author_facet Ayman Alzu’bi (13162392)
Ejlal Abu-El-Rub (5168183)
Fatimah A. Almahasneh (22224547)
Rawan Almazari (21842705)
Amani Kasasbeh (22330402)
Heba F. AI-jariri (22224550)
Amneh Alrabie (23073376)
Raed M. Al-Zoubi (2037490)
author_role author
dc.creator.none.fl_str_mv Ayman Alzu’bi (13162392)
Ejlal Abu-El-Rub (5168183)
Fatimah A. Almahasneh (22224547)
Rawan Almazari (21842705)
Amani Kasasbeh (22330402)
Heba F. AI-jariri (22224550)
Amneh Alrabie (23073376)
Raed M. Al-Zoubi (2037490)
dc.date.none.fl_str_mv 2025-10-13T09:00:00Z
dc.identifier.none.fl_str_mv 10.1007/s11419-025-00739-y
dc.relation.none.fl_str_mv https://figshare.com/articles/journal_contribution/Synthetic_Cannabinoid_AB-FUBINACA_Negatively_Impacted_the_Male_Fertility_and_Induced_Testicular_Toxicity/31168612
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Biomedical and clinical sciences
Medical biochemistry and metabolomics
Pharmacology and pharmaceutical sciences
Synthetic cannabinoids
AB-FUBINACA
Male fertility
Oxidative stress
Apoptosis
dc.title.none.fl_str_mv Synthetic Cannabinoid AB-FUBINACA Negatively Impacted the Male Fertility and Induced Testicular Toxicity
dc.type.none.fl_str_mv Text
Journal contribution
info:eu-repo/semantics/publishedVersion
text
contribution to journal
description <h3 dir="ltr">Purpose</h3><p dir="ltr">The recreational use of synthetic cannabinoids (SCs) by adolescents and adults has markedly increased in recent years. Previous studies demonstrated that exposure to SCs is associated with multiple adverse health effects. Nevertheless, little is known about the effects of these substances on male fertility. The current study aimed to investigate the toxicological effects of subacute exposure to synthetic cannabinoid AB-FUBINACA on male reproductive system in mice.</p><h3 dir="ltr">Methods</h3><p dir="ltr">Adult male Balb/c mice received daily intraperitoneal injections of various doses of AB-FUBINACA (0.75, 1.5, and 3 mg/kg for 3 weeks). Using biochemical and molecular methodologies, the impact of AB-FUBINACA on serum levels of reproductive hormones, sperm viability as well as various parameters in testicular tissue were evaluated.</p><h3 dir="ltr">Results</h3><p dir="ltr">Our findings demonstrated that AB-FUBINACA induces dose-dependent reduction in testosterone levels in the serum, but not in follicle-stimulating hormone or luteinizing hormone. AB-FUBINACA treatment also causes a significant dose related decrease in sperm viability. These findings were associated with higher level of oxidative stress (GP91 expression and malondialdehyde level) and elevated expression of key regulators of apoptosis (Bax and caspase-3) as well as reduced expression of mitochondrial respiratory chain complexes SDHB (II), UQCRC2 (III), and ATP5a (V) in the testicular tissue.</p><h3 dir="ltr">Conclusion</h3><p dir="ltr">From these findings, it can be concluded that exposure to AB-FUBINACA can interfere with the normal physiology and functioning of the male reproductive organs. Hence, gaining insight into the mechanisms by which SCs interfere with male fertility could guide future interventions and treatments.</p><h2 dir="ltr">Other Information</h2><p dir="ltr">Published in: Forensic Toxicology<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1007/s11419-025-00739-y" target="_blank">https://dx.doi.org/10.1007/s11419-025-00739-y</a></p>
eu_rights_str_mv openAccess
id Manara2_6a3b3437122b1a004fe0f0f2173c4903
identifier_str_mv 10.1007/s11419-025-00739-y
network_acronym_str Manara2
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oai_identifier_str oai:figshare.com:article/31168612
publishDate 2025
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rights_invalid_str_mv CC BY 4.0
spelling Synthetic Cannabinoid AB-FUBINACA Negatively Impacted the Male Fertility and Induced Testicular ToxicityAyman Alzu’bi (13162392)Ejlal Abu-El-Rub (5168183)Fatimah A. Almahasneh (22224547)Rawan Almazari (21842705)Amani Kasasbeh (22330402)Heba F. AI-jariri (22224550)Amneh Alrabie (23073376)Raed M. Al-Zoubi (2037490)Biomedical and clinical sciencesMedical biochemistry and metabolomicsPharmacology and pharmaceutical sciencesSynthetic cannabinoidsAB-FUBINACAMale fertilityOxidative stressApoptosis<h3 dir="ltr">Purpose</h3><p dir="ltr">The recreational use of synthetic cannabinoids (SCs) by adolescents and adults has markedly increased in recent years. Previous studies demonstrated that exposure to SCs is associated with multiple adverse health effects. Nevertheless, little is known about the effects of these substances on male fertility. The current study aimed to investigate the toxicological effects of subacute exposure to synthetic cannabinoid AB-FUBINACA on male reproductive system in mice.</p><h3 dir="ltr">Methods</h3><p dir="ltr">Adult male Balb/c mice received daily intraperitoneal injections of various doses of AB-FUBINACA (0.75, 1.5, and 3 mg/kg for 3 weeks). Using biochemical and molecular methodologies, the impact of AB-FUBINACA on serum levels of reproductive hormones, sperm viability as well as various parameters in testicular tissue were evaluated.</p><h3 dir="ltr">Results</h3><p dir="ltr">Our findings demonstrated that AB-FUBINACA induces dose-dependent reduction in testosterone levels in the serum, but not in follicle-stimulating hormone or luteinizing hormone. AB-FUBINACA treatment also causes a significant dose related decrease in sperm viability. These findings were associated with higher level of oxidative stress (GP91 expression and malondialdehyde level) and elevated expression of key regulators of apoptosis (Bax and caspase-3) as well as reduced expression of mitochondrial respiratory chain complexes SDHB (II), UQCRC2 (III), and ATP5a (V) in the testicular tissue.</p><h3 dir="ltr">Conclusion</h3><p dir="ltr">From these findings, it can be concluded that exposure to AB-FUBINACA can interfere with the normal physiology and functioning of the male reproductive organs. Hence, gaining insight into the mechanisms by which SCs interfere with male fertility could guide future interventions and treatments.</p><h2 dir="ltr">Other Information</h2><p dir="ltr">Published in: Forensic Toxicology<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1007/s11419-025-00739-y" target="_blank">https://dx.doi.org/10.1007/s11419-025-00739-y</a></p>2025-10-13T09:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1007/s11419-025-00739-yhttps://figshare.com/articles/journal_contribution/Synthetic_Cannabinoid_AB-FUBINACA_Negatively_Impacted_the_Male_Fertility_and_Induced_Testicular_Toxicity/31168612CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/311686122025-10-13T09:00:00Z
spellingShingle Synthetic Cannabinoid AB-FUBINACA Negatively Impacted the Male Fertility and Induced Testicular Toxicity
Ayman Alzu’bi (13162392)
Biomedical and clinical sciences
Medical biochemistry and metabolomics
Pharmacology and pharmaceutical sciences
Synthetic cannabinoids
AB-FUBINACA
Male fertility
Oxidative stress
Apoptosis
status_str publishedVersion
title Synthetic Cannabinoid AB-FUBINACA Negatively Impacted the Male Fertility and Induced Testicular Toxicity
title_full Synthetic Cannabinoid AB-FUBINACA Negatively Impacted the Male Fertility and Induced Testicular Toxicity
title_fullStr Synthetic Cannabinoid AB-FUBINACA Negatively Impacted the Male Fertility and Induced Testicular Toxicity
title_full_unstemmed Synthetic Cannabinoid AB-FUBINACA Negatively Impacted the Male Fertility and Induced Testicular Toxicity
title_short Synthetic Cannabinoid AB-FUBINACA Negatively Impacted the Male Fertility and Induced Testicular Toxicity
title_sort Synthetic Cannabinoid AB-FUBINACA Negatively Impacted the Male Fertility and Induced Testicular Toxicity
topic Biomedical and clinical sciences
Medical biochemistry and metabolomics
Pharmacology and pharmaceutical sciences
Synthetic cannabinoids
AB-FUBINACA
Male fertility
Oxidative stress
Apoptosis