Physical Exercise Enhanced Heat Shock Protein 60 Expression and Attenuated Inflammation in the Adipose Tissue of Human Diabetic Obese
<p dir="ltr">Heat shock protein 60 (HSP60) is a key protein in the crosstalk between cellular stress and inflammation. However, the status of HSP60 in diabetes and obesity is unclear. In the present study, we investigated the hypothesis that HSP60 expression levels in the adipose tis...
محفوظ في:
| المؤلف الرئيسي: | |
|---|---|
| مؤلفون آخرون: | , , , , , |
| منشور في: |
2018
|
| الموضوعات: | |
| الوسوم: |
إضافة وسم
لا توجد وسوم, كن أول من يضع وسما على هذه التسجيلة!
|
| _version_ | 1864513513390604288 |
|---|---|
| author | Abdelkrim Khadir (437074) |
| author2 | Sina Kavalakatt (437073) Preethi Cherian (437070) Samia Warsame (437075) Jehad Ahmed Abubaker (6268541) Mohammed Dehbi (309033) Ali Tiss (437068) |
| author2_role | author author author author author author |
| author_facet | Abdelkrim Khadir (437074) Sina Kavalakatt (437073) Preethi Cherian (437070) Samia Warsame (437075) Jehad Ahmed Abubaker (6268541) Mohammed Dehbi (309033) Ali Tiss (437068) |
| author_role | author |
| dc.creator.none.fl_str_mv | Abdelkrim Khadir (437074) Sina Kavalakatt (437073) Preethi Cherian (437070) Samia Warsame (437075) Jehad Ahmed Abubaker (6268541) Mohammed Dehbi (309033) Ali Tiss (437068) |
| dc.date.none.fl_str_mv | 2018-02-06T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3389/fendo.2018.00016 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Physical_Exercise_Enhanced_Heat_Shock_Protein_60_Expression_and_Attenuated_Inflammation_in_the_Adipose_Tissue_of_Human_Diabetic_Obese/25921045 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Clinical sciences Medical biochemistry and metabolomics cellular stress heat shock response heat shock protein 60 physical exercise adipose tissue |
| dc.title.none.fl_str_mv | Physical Exercise Enhanced Heat Shock Protein 60 Expression and Attenuated Inflammation in the Adipose Tissue of Human Diabetic Obese |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Heat shock protein 60 (HSP60) is a key protein in the crosstalk between cellular stress and inflammation. However, the status of HSP60 in diabetes and obesity is unclear. In the present study, we investigated the hypothesis that HSP60 expression levels in the adipose tissue of human obese adults with and without diabetes are different and physical exercise might affect these levels. Subcutaneous adipose tissue (SAT) and blood samples were collected from obese adults with and without diabetes (<i>n</i> = 138 and <i>n</i> = 92, respectively, at baseline; <i>n</i> = 43 for both groups after 3 months of physical exercise). Conventional RT-PCR, immunohistochemistry, immunofluorescence, and ELISA were used to assess the expression and secretion of HSP60. Compared with obese adults without diabetes, HSP60 mRNA and protein levels were decreased in SAT in diabetic obese together with increased inflammatory marker expression and glycemic levels but lower VO<sub>2 Max</sub>. More interestingly, a 3-month physical exercise differentially affected HSP60 expression and the heat shock response but attenuated inflammation in both groups, as reflected by decreased endogenous levels of IL-6 and TNF-α. Indeed, HSP60 expression levels in SAT were significantly increased by exercise in the diabetes group, whereas they were decreased in the non-diabetes group. These results were further confirmed using immunofluorescence microscopy and anti-HSP60 antibody in SAT. Exercise had only marginal effects on HSP60 secretion and HSP60 autoantibody levels in plasma in both obese with and without diabetes. Physical exercise differentially alleviates cellular stress in obese adults with and without diabetes despite concomitant attenuation of the inflammatory response.</p><h2>Other Information</h2><p dir="ltr">Published in: Frontiers in Endocrinology<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3389/fendo.2018.00016" target="_blank">https://dx.doi.org/10.3389/fendo.2018.00016</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_6ab93685e7789162877f60f630076fc3 |
| identifier_str_mv | 10.3389/fendo.2018.00016 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25921045 |
| publishDate | 2018 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Physical Exercise Enhanced Heat Shock Protein 60 Expression and Attenuated Inflammation in the Adipose Tissue of Human Diabetic ObeseAbdelkrim Khadir (437074)Sina Kavalakatt (437073)Preethi Cherian (437070)Samia Warsame (437075)Jehad Ahmed Abubaker (6268541)Mohammed Dehbi (309033)Ali Tiss (437068)Biomedical and clinical sciencesClinical sciencesMedical biochemistry and metabolomicscellular stressheat shock responseheat shock protein 60physical exerciseadipose tissue<p dir="ltr">Heat shock protein 60 (HSP60) is a key protein in the crosstalk between cellular stress and inflammation. However, the status of HSP60 in diabetes and obesity is unclear. In the present study, we investigated the hypothesis that HSP60 expression levels in the adipose tissue of human obese adults with and without diabetes are different and physical exercise might affect these levels. Subcutaneous adipose tissue (SAT) and blood samples were collected from obese adults with and without diabetes (<i>n</i> = 138 and <i>n</i> = 92, respectively, at baseline; <i>n</i> = 43 for both groups after 3 months of physical exercise). Conventional RT-PCR, immunohistochemistry, immunofluorescence, and ELISA were used to assess the expression and secretion of HSP60. Compared with obese adults without diabetes, HSP60 mRNA and protein levels were decreased in SAT in diabetic obese together with increased inflammatory marker expression and glycemic levels but lower VO<sub>2 Max</sub>. More interestingly, a 3-month physical exercise differentially affected HSP60 expression and the heat shock response but attenuated inflammation in both groups, as reflected by decreased endogenous levels of IL-6 and TNF-α. Indeed, HSP60 expression levels in SAT were significantly increased by exercise in the diabetes group, whereas they were decreased in the non-diabetes group. These results were further confirmed using immunofluorescence microscopy and anti-HSP60 antibody in SAT. Exercise had only marginal effects on HSP60 secretion and HSP60 autoantibody levels in plasma in both obese with and without diabetes. Physical exercise differentially alleviates cellular stress in obese adults with and without diabetes despite concomitant attenuation of the inflammatory response.</p><h2>Other Information</h2><p dir="ltr">Published in: Frontiers in Endocrinology<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3389/fendo.2018.00016" target="_blank">https://dx.doi.org/10.3389/fendo.2018.00016</a></p>2018-02-06T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3389/fendo.2018.00016https://figshare.com/articles/journal_contribution/Physical_Exercise_Enhanced_Heat_Shock_Protein_60_Expression_and_Attenuated_Inflammation_in_the_Adipose_Tissue_of_Human_Diabetic_Obese/25921045CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/259210452018-02-06T03:00:00Z |
| spellingShingle | Physical Exercise Enhanced Heat Shock Protein 60 Expression and Attenuated Inflammation in the Adipose Tissue of Human Diabetic Obese Abdelkrim Khadir (437074) Biomedical and clinical sciences Clinical sciences Medical biochemistry and metabolomics cellular stress heat shock response heat shock protein 60 physical exercise adipose tissue |
| status_str | publishedVersion |
| title | Physical Exercise Enhanced Heat Shock Protein 60 Expression and Attenuated Inflammation in the Adipose Tissue of Human Diabetic Obese |
| title_full | Physical Exercise Enhanced Heat Shock Protein 60 Expression and Attenuated Inflammation in the Adipose Tissue of Human Diabetic Obese |
| title_fullStr | Physical Exercise Enhanced Heat Shock Protein 60 Expression and Attenuated Inflammation in the Adipose Tissue of Human Diabetic Obese |
| title_full_unstemmed | Physical Exercise Enhanced Heat Shock Protein 60 Expression and Attenuated Inflammation in the Adipose Tissue of Human Diabetic Obese |
| title_short | Physical Exercise Enhanced Heat Shock Protein 60 Expression and Attenuated Inflammation in the Adipose Tissue of Human Diabetic Obese |
| title_sort | Physical Exercise Enhanced Heat Shock Protein 60 Expression and Attenuated Inflammation in the Adipose Tissue of Human Diabetic Obese |
| topic | Biomedical and clinical sciences Clinical sciences Medical biochemistry and metabolomics cellular stress heat shock response heat shock protein 60 physical exercise adipose tissue |