Northern blotting of endogenous full-length human-specific LINE-1 RNA
<p dir="ltr">LINE-1 belongs to a family of DNA elements that move to new locations in the genome in a process called “retrotransposition.” This is achieved by a copy-and-paste mechanism with the aid of an RNA intermediate. The full-length LINE-1 is responsible for most retrotransposi...
محفوظ في:
| المؤلف الرئيسي: | |
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| منشور في: |
2024
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| الموضوعات: | |
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إضافة وسم
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| الملخص: | <p dir="ltr">LINE-1 belongs to a family of DNA elements that move to new locations in the genome in a process called “retrotransposition.” This is achieved by a copy-and-paste mechanism with the aid of an RNA intermediate. The full-length LINE-1 is responsible for most retrotransposition activity in the human genome. Detecting the active LINE-1 RNA at the endogenous level is challenging due to its small percentage among inactive copies and its different forms of transcripts. Here, we describe a method of designing RNA probes to detect active LINE-1 by northern blotting and use optimized conditions and tools to make the detection practical. This method uses a classical long RNA probe and provides an alternative way to detect LINE-1 RNA using multiple short RNA probes.</p><h2>Other Information</h2><p dir="ltr">Published in: Biology Methods and Protocols<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1093/biomethods/bpae036" target="_blank">https://dx.doi.org/10.1093/biomethods/bpae036</a></p> |
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