Northern blotting of endogenous full-length human-specific LINE-1 RNA
<p dir="ltr">LINE-1 belongs to a family of DNA elements that move to new locations in the genome in a process called “retrotransposition.” This is achieved by a copy-and-paste mechanism with the aid of an RNA intermediate. The full-length LINE-1 is responsible for most retrotransposi...
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2024
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| _version_ | 1864513542147801088 |
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| author | Maisa I Alkailani (21842354) |
| author_facet | Maisa I Alkailani (21842354) |
| author_role | author |
| dc.creator.none.fl_str_mv | Maisa I Alkailani (21842354) |
| dc.date.none.fl_str_mv | 2024-05-28T09:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1093/biomethods/bpae036 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Northern_blotting_of_endogenous_full-length_human-specific_LINE-1_RNA/29715812 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Genetics LINE-1 Retrotransposition Transposable elements RNA detection Northern blotting RNA probes |
| dc.title.none.fl_str_mv | Northern blotting of endogenous full-length human-specific LINE-1 RNA |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">LINE-1 belongs to a family of DNA elements that move to new locations in the genome in a process called “retrotransposition.” This is achieved by a copy-and-paste mechanism with the aid of an RNA intermediate. The full-length LINE-1 is responsible for most retrotransposition activity in the human genome. Detecting the active LINE-1 RNA at the endogenous level is challenging due to its small percentage among inactive copies and its different forms of transcripts. Here, we describe a method of designing RNA probes to detect active LINE-1 by northern blotting and use optimized conditions and tools to make the detection practical. This method uses a classical long RNA probe and provides an alternative way to detect LINE-1 RNA using multiple short RNA probes.</p><h2>Other Information</h2><p dir="ltr">Published in: Biology Methods and Protocols<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1093/biomethods/bpae036" target="_blank">https://dx.doi.org/10.1093/biomethods/bpae036</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_6b5837f77136a3dabd90b7963d3b8145 |
| identifier_str_mv | 10.1093/biomethods/bpae036 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/29715812 |
| publishDate | 2024 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Northern blotting of endogenous full-length human-specific LINE-1 RNAMaisa I Alkailani (21842354)Biological sciencesGeneticsLINE-1RetrotranspositionTransposable elementsRNA detectionNorthern blottingRNA probes<p dir="ltr">LINE-1 belongs to a family of DNA elements that move to new locations in the genome in a process called “retrotransposition.” This is achieved by a copy-and-paste mechanism with the aid of an RNA intermediate. The full-length LINE-1 is responsible for most retrotransposition activity in the human genome. Detecting the active LINE-1 RNA at the endogenous level is challenging due to its small percentage among inactive copies and its different forms of transcripts. Here, we describe a method of designing RNA probes to detect active LINE-1 by northern blotting and use optimized conditions and tools to make the detection practical. This method uses a classical long RNA probe and provides an alternative way to detect LINE-1 RNA using multiple short RNA probes.</p><h2>Other Information</h2><p dir="ltr">Published in: Biology Methods and Protocols<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1093/biomethods/bpae036" target="_blank">https://dx.doi.org/10.1093/biomethods/bpae036</a></p>2024-05-28T09:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1093/biomethods/bpae036https://figshare.com/articles/journal_contribution/Northern_blotting_of_endogenous_full-length_human-specific_LINE-1_RNA/29715812CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/297158122024-05-28T09:00:00Z |
| spellingShingle | Northern blotting of endogenous full-length human-specific LINE-1 RNA Maisa I Alkailani (21842354) Biological sciences Genetics LINE-1 Retrotransposition Transposable elements RNA detection Northern blotting RNA probes |
| status_str | publishedVersion |
| title | Northern blotting of endogenous full-length human-specific LINE-1 RNA |
| title_full | Northern blotting of endogenous full-length human-specific LINE-1 RNA |
| title_fullStr | Northern blotting of endogenous full-length human-specific LINE-1 RNA |
| title_full_unstemmed | Northern blotting of endogenous full-length human-specific LINE-1 RNA |
| title_short | Northern blotting of endogenous full-length human-specific LINE-1 RNA |
| title_sort | Northern blotting of endogenous full-length human-specific LINE-1 RNA |
| topic | Biological sciences Genetics LINE-1 Retrotransposition Transposable elements RNA detection Northern blotting RNA probes |