Ecotoxicological Assessment of Thermally- and Hydrogen-Reduced Graphene Oxide/TiO<sub>2</sub> Photocatalytic Nanocomposites Using the Zebrafish Embryo Model

<p dir="ltr">Advanced oxidation processes (AOPs) have recently attracted great interest in water pollution management. Using the zebrafish embryo model, we investigated the environmental impacts of two thermally (RGOTi)- and hydrogen (H<sub>2</sub>RGOTi)-reduced graphene...

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Main Author: Halema Al-Kandari (6316961) (author)
Other Authors: Nadin Younes (4863280) (author), Ola Al-Jamal (11721377) (author), Zain Z. Zakaria (6695093) (author), Huda Najjar (18134101) (author), Farah Alserr (18134104) (author), Gianfranco Pintus (91638) (author), Maha A. Al-Asmakh (17128744) (author), Aboubakr M. Abdullah (1505017) (author), Gheyath K. Nasrallah (9200525) (author)
Published: 2019
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_version_ 1864513521775017984
author Halema Al-Kandari (6316961)
author2 Nadin Younes (4863280)
Ola Al-Jamal (11721377)
Zain Z. Zakaria (6695093)
Huda Najjar (18134101)
Farah Alserr (18134104)
Gianfranco Pintus (91638)
Maha A. Al-Asmakh (17128744)
Aboubakr M. Abdullah (1505017)
Gheyath K. Nasrallah (9200525)
author2_role author
author
author
author
author
author
author
author
author
author_facet Halema Al-Kandari (6316961)
Nadin Younes (4863280)
Ola Al-Jamal (11721377)
Zain Z. Zakaria (6695093)
Huda Najjar (18134101)
Farah Alserr (18134104)
Gianfranco Pintus (91638)
Maha A. Al-Asmakh (17128744)
Aboubakr M. Abdullah (1505017)
Gheyath K. Nasrallah (9200525)
author_role author
dc.creator.none.fl_str_mv Halema Al-Kandari (6316961)
Nadin Younes (4863280)
Ola Al-Jamal (11721377)
Zain Z. Zakaria (6695093)
Huda Najjar (18134101)
Farah Alserr (18134104)
Gianfranco Pintus (91638)
Maha A. Al-Asmakh (17128744)
Aboubakr M. Abdullah (1505017)
Gheyath K. Nasrallah (9200525)
dc.date.none.fl_str_mv 2019-03-28T03:00:00Z
dc.identifier.none.fl_str_mv 10.3390/nano9040488
dc.relation.none.fl_str_mv https://figshare.com/articles/journal_contribution/Ecotoxicological_Assessment_of_Thermally-_and_Hydrogen-Reduced_Graphene_Oxide_TiO_sub_2_sub_Photocatalytic_Nanocomposites_Using_the_Zebrafish_Embryo_Model/25378849
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Engineering
Chemical engineering
Materials engineering
reduced graphene oxide/TiO2
nanocomposite photocatalysts
zebrafish
toxicity
LC50
dc.title.none.fl_str_mv Ecotoxicological Assessment of Thermally- and Hydrogen-Reduced Graphene Oxide/TiO<sub>2</sub> Photocatalytic Nanocomposites Using the Zebrafish Embryo Model
dc.type.none.fl_str_mv Text
Journal contribution
info:eu-repo/semantics/publishedVersion
text
contribution to journal
description <p dir="ltr">Advanced oxidation processes (AOPs) have recently attracted great interest in water pollution management. Using the zebrafish embryo model, we investigated the environmental impacts of two thermally (RGOTi)- and hydrogen (H<sub>2</sub>RGOTi)-reduced graphene oxide/TiO<sub>2</sub> semiconductor photocatalysts recently employed in AOPs. For this purpose, acutoxicity, cardiotoxicity, neurobehavioral toxicity, hematopoietic toxicity, and hatching rate were determinate. For the RGOTi, the no observed effect concentration (NOEC, mortality/teratogenicity score <20%) and the median lethal concentration (LC<sub>50</sub>) were <400 and 748.6 mg/L, respectively. H<sub>2</sub>RGOTi showed a NOEC similar to RGOTi. However, no significant mortality was detected at all concentrations used in the acutoxicity assay (up to1000 mg/L), thus indicating a hypothetical LC<sub>50</sub> higher than 1000 mg/L. According to the Fish and Wildlife Service Acute Toxicity Rating Scale, RGOTi can be classified as “practically not toxic” and H<sub>2</sub>RGOTi as “relatively harmless”. However, both nanocomposites should be used with caution at concentration higher than the NOEC (400 mg/L), in particular RGOTi, which significantly (i) caused pericardial and yolk sac edema; (ii) decreased the hatching rate, locomotion, and hematopoietic activities; and (iii) affected the heart rate. Indeed, the aforementioned teratogenic phenotypes were less devastating in H<sub>2</sub>RGOTi-treated embryos, suggesting that the hydrogen-reduced graphene oxide/TiO<sub>2</sub> photocatalysts may be more ecofriendly than the thermally-reduced ones.</p><p dir="ltr"><br></p><h2>Other Information</h2><p dir="ltr">Published in: Nanomaterials<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/nano9040488" target="_blank">https://dx.doi.org/10.3390/nano9040488</a></p>
eu_rights_str_mv openAccess
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identifier_str_mv 10.3390/nano9040488
network_acronym_str Manara2
network_name_str Manara2
oai_identifier_str oai:figshare.com:article/25378849
publishDate 2019
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spelling Ecotoxicological Assessment of Thermally- and Hydrogen-Reduced Graphene Oxide/TiO<sub>2</sub> Photocatalytic Nanocomposites Using the Zebrafish Embryo ModelHalema Al-Kandari (6316961)Nadin Younes (4863280)Ola Al-Jamal (11721377)Zain Z. Zakaria (6695093)Huda Najjar (18134101)Farah Alserr (18134104)Gianfranco Pintus (91638)Maha A. Al-Asmakh (17128744)Aboubakr M. Abdullah (1505017)Gheyath K. Nasrallah (9200525)EngineeringChemical engineeringMaterials engineeringreduced graphene oxide/TiO2nanocomposite photocatalystszebrafishtoxicityLC50<p dir="ltr">Advanced oxidation processes (AOPs) have recently attracted great interest in water pollution management. Using the zebrafish embryo model, we investigated the environmental impacts of two thermally (RGOTi)- and hydrogen (H<sub>2</sub>RGOTi)-reduced graphene oxide/TiO<sub>2</sub> semiconductor photocatalysts recently employed in AOPs. For this purpose, acutoxicity, cardiotoxicity, neurobehavioral toxicity, hematopoietic toxicity, and hatching rate were determinate. For the RGOTi, the no observed effect concentration (NOEC, mortality/teratogenicity score <20%) and the median lethal concentration (LC<sub>50</sub>) were <400 and 748.6 mg/L, respectively. H<sub>2</sub>RGOTi showed a NOEC similar to RGOTi. However, no significant mortality was detected at all concentrations used in the acutoxicity assay (up to1000 mg/L), thus indicating a hypothetical LC<sub>50</sub> higher than 1000 mg/L. According to the Fish and Wildlife Service Acute Toxicity Rating Scale, RGOTi can be classified as “practically not toxic” and H<sub>2</sub>RGOTi as “relatively harmless”. However, both nanocomposites should be used with caution at concentration higher than the NOEC (400 mg/L), in particular RGOTi, which significantly (i) caused pericardial and yolk sac edema; (ii) decreased the hatching rate, locomotion, and hematopoietic activities; and (iii) affected the heart rate. Indeed, the aforementioned teratogenic phenotypes were less devastating in H<sub>2</sub>RGOTi-treated embryos, suggesting that the hydrogen-reduced graphene oxide/TiO<sub>2</sub> photocatalysts may be more ecofriendly than the thermally-reduced ones.</p><p dir="ltr"><br></p><h2>Other Information</h2><p dir="ltr">Published in: Nanomaterials<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/nano9040488" target="_blank">https://dx.doi.org/10.3390/nano9040488</a></p>2019-03-28T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3390/nano9040488https://figshare.com/articles/journal_contribution/Ecotoxicological_Assessment_of_Thermally-_and_Hydrogen-Reduced_Graphene_Oxide_TiO_sub_2_sub_Photocatalytic_Nanocomposites_Using_the_Zebrafish_Embryo_Model/25378849CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/253788492019-03-28T03:00:00Z
spellingShingle Ecotoxicological Assessment of Thermally- and Hydrogen-Reduced Graphene Oxide/TiO<sub>2</sub> Photocatalytic Nanocomposites Using the Zebrafish Embryo Model
Halema Al-Kandari (6316961)
Engineering
Chemical engineering
Materials engineering
reduced graphene oxide/TiO2
nanocomposite photocatalysts
zebrafish
toxicity
LC50
status_str publishedVersion
title Ecotoxicological Assessment of Thermally- and Hydrogen-Reduced Graphene Oxide/TiO<sub>2</sub> Photocatalytic Nanocomposites Using the Zebrafish Embryo Model
title_full Ecotoxicological Assessment of Thermally- and Hydrogen-Reduced Graphene Oxide/TiO<sub>2</sub> Photocatalytic Nanocomposites Using the Zebrafish Embryo Model
title_fullStr Ecotoxicological Assessment of Thermally- and Hydrogen-Reduced Graphene Oxide/TiO<sub>2</sub> Photocatalytic Nanocomposites Using the Zebrafish Embryo Model
title_full_unstemmed Ecotoxicological Assessment of Thermally- and Hydrogen-Reduced Graphene Oxide/TiO<sub>2</sub> Photocatalytic Nanocomposites Using the Zebrafish Embryo Model
title_short Ecotoxicological Assessment of Thermally- and Hydrogen-Reduced Graphene Oxide/TiO<sub>2</sub> Photocatalytic Nanocomposites Using the Zebrafish Embryo Model
title_sort Ecotoxicological Assessment of Thermally- and Hydrogen-Reduced Graphene Oxide/TiO<sub>2</sub> Photocatalytic Nanocomposites Using the Zebrafish Embryo Model
topic Engineering
Chemical engineering
Materials engineering
reduced graphene oxide/TiO2
nanocomposite photocatalysts
zebrafish
toxicity
LC50