β-lactamase-mediated resistance in MDR-Pseudomonas aeruginosa from Qatar
<h3>Background</h3><p dir="ltr">The distribution of β-lactam resistance genes in P. aeruginosa is often closely related to the distribution of certain high-risk international clones. We used whole-genome sequencing (WGS) to identify the predominant sequence types (ST) and...
محفوظ في:
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| مؤلفون آخرون: | , , , , , |
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2020
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| _version_ | 1864513511790477312 |
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| author | Mazen A. Sid Ahmed (18810337) |
| author2 | Faisal Ahmad Khan (7832540) Ali A. Sultan (7409735) Bo Söderquist (466146) Emad Bashir Ibrahim (9590113) Jana Jass (677208) Ali S. Omrani (9590116) |
| author2_role | author author author author author author |
| author_facet | Mazen A. Sid Ahmed (18810337) Faisal Ahmad Khan (7832540) Ali A. Sultan (7409735) Bo Söderquist (466146) Emad Bashir Ibrahim (9590113) Jana Jass (677208) Ali S. Omrani (9590116) |
| author_role | author |
| dc.creator.none.fl_str_mv | Mazen A. Sid Ahmed (18810337) Faisal Ahmad Khan (7832540) Ali A. Sultan (7409735) Bo Söderquist (466146) Emad Bashir Ibrahim (9590113) Jana Jass (677208) Ali S. Omrani (9590116) |
| dc.date.none.fl_str_mv | 2020-11-01T00:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1186/s13756-020-00838-y |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/_-lactamase-mediated_resistance_in_MDR-Pseudomonas_aeruginosa_from_Qatar/26020933 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Genetics Biomedical and clinical sciences Medical microbiology Beta-lactamases MDR P. aeruginosa ST235 Beta-lactamases MDR P. aeruginosa ST235 |
| dc.title.none.fl_str_mv | β-lactamase-mediated resistance in MDR-Pseudomonas aeruginosa from Qatar |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <h3>Background</h3><p dir="ltr">The distribution of β-lactam resistance genes in P. aeruginosa is often closely related to the distribution of certain high-risk international clones. We used whole-genome sequencing (WGS) to identify the predominant sequence types (ST) and β-lactamase genes in clinical isolates of multidrug-resistant (MDR)-P. aeruginosa from Qatar</p><h3>Methods</h3><p dir="ltr">Microbiological identification and susceptibility tests were performed by automated BD Phoenix™ system and manual Liofilchem MIC Test Strips.</p><h3>Results</h3><p dir="ltr">Among 75 MDR-P. aeruginosa isolates; the largest proportions of susceptibility were to ceftazidime-avibactam (n = 36, 48%), followed by ceftolozane-tazobactam (30, 40%), ceftazidime (n = 21, 28%) and aztreonam (n = 16, 21.3%). All isolates possessed Class C and/or Class D β-lactamases (n = 72, 96% each), while metallo-β-lactamases were detected in 20 (26.7%) isolates. Eight (40%) metallo-β-lactamase producers were susceptible to aztreonam and did not produce any concomitant extended-spectrum β-lactamases. High risk ST235 (n = 16, 21.3%), ST357 (n = 8, 10.7%), ST389 and ST1284 (6, 8% each) were most frequent. Nearly all ST235 isolates (15/16; 93.8%) were resistant to all tested β-lactams.</p><h3>Conclusion</h3><p dir="ltr">MDR-P. aeruginosa isolates from Qatar are highly resistant to antipseudomonal β-lactams. High-risk STs are predominant in Qatar and their associated MDR phenotypes are a cause for considerable concern.</p><h2>Other Information</h2><p dir="ltr">Published in: Antimicrobial Resistance & Infection Control<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1186/s13756-020-00838-y" target="_blank">https://dx.doi.org/10.1186/s13756-020-00838-y</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_6d2df9f2ae429e746c909dffed28f0d8 |
| identifier_str_mv | 10.1186/s13756-020-00838-y |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/26020933 |
| publishDate | 2020 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | β-lactamase-mediated resistance in MDR-Pseudomonas aeruginosa from QatarMazen A. Sid Ahmed (18810337)Faisal Ahmad Khan (7832540)Ali A. Sultan (7409735)Bo Söderquist (466146)Emad Bashir Ibrahim (9590113)Jana Jass (677208)Ali S. Omrani (9590116)Biological sciencesGeneticsBiomedical and clinical sciencesMedical microbiologyBeta-lactamases MDR P. aeruginosa ST235Beta-lactamasesMDRP. aeruginosaST235<h3>Background</h3><p dir="ltr">The distribution of β-lactam resistance genes in P. aeruginosa is often closely related to the distribution of certain high-risk international clones. We used whole-genome sequencing (WGS) to identify the predominant sequence types (ST) and β-lactamase genes in clinical isolates of multidrug-resistant (MDR)-P. aeruginosa from Qatar</p><h3>Methods</h3><p dir="ltr">Microbiological identification and susceptibility tests were performed by automated BD Phoenix™ system and manual Liofilchem MIC Test Strips.</p><h3>Results</h3><p dir="ltr">Among 75 MDR-P. aeruginosa isolates; the largest proportions of susceptibility were to ceftazidime-avibactam (n = 36, 48%), followed by ceftolozane-tazobactam (30, 40%), ceftazidime (n = 21, 28%) and aztreonam (n = 16, 21.3%). All isolates possessed Class C and/or Class D β-lactamases (n = 72, 96% each), while metallo-β-lactamases were detected in 20 (26.7%) isolates. Eight (40%) metallo-β-lactamase producers were susceptible to aztreonam and did not produce any concomitant extended-spectrum β-lactamases. High risk ST235 (n = 16, 21.3%), ST357 (n = 8, 10.7%), ST389 and ST1284 (6, 8% each) were most frequent. Nearly all ST235 isolates (15/16; 93.8%) were resistant to all tested β-lactams.</p><h3>Conclusion</h3><p dir="ltr">MDR-P. aeruginosa isolates from Qatar are highly resistant to antipseudomonal β-lactams. High-risk STs are predominant in Qatar and their associated MDR phenotypes are a cause for considerable concern.</p><h2>Other Information</h2><p dir="ltr">Published in: Antimicrobial Resistance & Infection Control<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1186/s13756-020-00838-y" target="_blank">https://dx.doi.org/10.1186/s13756-020-00838-y</a></p>2020-11-01T00:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1186/s13756-020-00838-yhttps://figshare.com/articles/journal_contribution/_-lactamase-mediated_resistance_in_MDR-Pseudomonas_aeruginosa_from_Qatar/26020933CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/260209332020-11-01T00:00:00Z |
| spellingShingle | β-lactamase-mediated resistance in MDR-Pseudomonas aeruginosa from Qatar Mazen A. Sid Ahmed (18810337) Biological sciences Genetics Biomedical and clinical sciences Medical microbiology Beta-lactamases MDR P. aeruginosa ST235 Beta-lactamases MDR P. aeruginosa ST235 |
| status_str | publishedVersion |
| title | β-lactamase-mediated resistance in MDR-Pseudomonas aeruginosa from Qatar |
| title_full | β-lactamase-mediated resistance in MDR-Pseudomonas aeruginosa from Qatar |
| title_fullStr | β-lactamase-mediated resistance in MDR-Pseudomonas aeruginosa from Qatar |
| title_full_unstemmed | β-lactamase-mediated resistance in MDR-Pseudomonas aeruginosa from Qatar |
| title_short | β-lactamase-mediated resistance in MDR-Pseudomonas aeruginosa from Qatar |
| title_sort | β-lactamase-mediated resistance in MDR-Pseudomonas aeruginosa from Qatar |
| topic | Biological sciences Genetics Biomedical and clinical sciences Medical microbiology Beta-lactamases MDR P. aeruginosa ST235 Beta-lactamases MDR P. aeruginosa ST235 |