Genetic Diversity and Prevalence of Giardia duodenalis in Qatar
<h3>Background</h3><p dir="ltr">Giardia duodenalis is a common human intestinal parasite worldwide, and the causative agent of diarrhea, with the severity of disease ranging from asymptomatic to intense and debilitating infection. G. duodenalis is known to consist of eigh...
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| مؤلفون آخرون: | , , , , , |
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2021
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| _version_ | 1864513511754825728 |
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| author | Marwa Chourabi (10742520) |
| author2 | Sonia Boughattas (508422) Atiyeh M. Abdallah (10742523) Ahmed Ismail (2671822) Jerzy M. Behnke (7571882) Hesham M. Al-Mekhlafi (10001903) Marawan Abu-Madi (2816869) |
| author2_role | author author author author author author |
| author_facet | Marwa Chourabi (10742520) Sonia Boughattas (508422) Atiyeh M. Abdallah (10742523) Ahmed Ismail (2671822) Jerzy M. Behnke (7571882) Hesham M. Al-Mekhlafi (10001903) Marawan Abu-Madi (2816869) |
| author_role | author |
| dc.creator.none.fl_str_mv | Marwa Chourabi (10742520) Sonia Boughattas (508422) Atiyeh M. Abdallah (10742523) Ahmed Ismail (2671822) Jerzy M. Behnke (7571882) Hesham M. Al-Mekhlafi (10001903) Marawan Abu-Madi (2816869) |
| dc.date.none.fl_str_mv | 2021-05-04T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3389/fcimb.2021.652946 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Genetic_Diversity_and_Prevalence_of_Giardia_duodenalis_in_Qatar/26020900 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Medical microbiology Health sciences Epidemiology G. duodenalis assemblages multilocus genotyping Qatar immigrant |
| dc.title.none.fl_str_mv | Genetic Diversity and Prevalence of Giardia duodenalis in Qatar |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <h3>Background</h3><p dir="ltr">Giardia duodenalis is a common human intestinal parasite worldwide, and the causative agent of diarrhea, with the severity of disease ranging from asymptomatic to intense and debilitating infection. G. duodenalis is known to consist of eight genetically distinct assemblages, named from A to H. No data available on the genotypes and genetic diversity of G. duodenalis circulating in Qatar.</p><h3>Methods</h3><p dir="ltr">We genotyped 54 human Giardia isolates, collected from asymptomatic immigrants in Qatar, using a multilocus genotyping (MLGs) tool. We also investigated relationships between the subjects’ genotypes and their demographic data.</p><h3>Results</h3><p dir="ltr">Genomic DNA from 54 isolates were tested by PCR and sequence analysis at three loci: glutamate dehydrogenase (gdh), β-giardin (bg) and triose phosphate (tpi)). Assemblage A was identified in nine (16.67%), assemblage B in thirty (55.55%), and a mixture of assemblages A+B in fifteen (27.78%) isolates. All assemblage A isolates, genotyped in different loci, were assigned to sub-assemblage AII, and six of them had MLGs AII-1 while one new MLG was identified in two isolates. Sequences of assemblage B isolates have high level of genetic diversity and high presence of heterogeneous peaks, especially within the gdh gene. No significant associations between genotypes and the immigrants’ demographic data were found due to the extensive number of new variants.</p><h3>Conclusions</h3><p dir="ltr">MLGs was used herein to genotype 54 immigrant Giardia isolates. The high level of genetic variability found in our isolates hampered MLGs determination, more investigations are now required to consolidate our findings, and to enable a comprehensive understanding of the diversity within G. duodenalis assemblage B isolates.</p><h2>Other Information</h2><p dir="ltr">Published in: Frontiers in Cellular and Infection Microbiology<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3389/fcimb.2021.652946" target="_blank">https://dx.doi.org/10.3389/fcimb.2021.652946</a></p><p dir="ltr">Additional institutions affiliated with: Medical Commission - MOPH</p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_6d36987e4d97c4658dea854606df14c3 |
| identifier_str_mv | 10.3389/fcimb.2021.652946 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/26020900 |
| publishDate | 2021 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Genetic Diversity and Prevalence of Giardia duodenalis in QatarMarwa Chourabi (10742520)Sonia Boughattas (508422)Atiyeh M. Abdallah (10742523)Ahmed Ismail (2671822)Jerzy M. Behnke (7571882)Hesham M. Al-Mekhlafi (10001903)Marawan Abu-Madi (2816869)Biomedical and clinical sciencesMedical microbiologyHealth sciencesEpidemiologyG. duodenalisassemblagesmultilocus genotypingQatarimmigrant<h3>Background</h3><p dir="ltr">Giardia duodenalis is a common human intestinal parasite worldwide, and the causative agent of diarrhea, with the severity of disease ranging from asymptomatic to intense and debilitating infection. G. duodenalis is known to consist of eight genetically distinct assemblages, named from A to H. No data available on the genotypes and genetic diversity of G. duodenalis circulating in Qatar.</p><h3>Methods</h3><p dir="ltr">We genotyped 54 human Giardia isolates, collected from asymptomatic immigrants in Qatar, using a multilocus genotyping (MLGs) tool. We also investigated relationships between the subjects’ genotypes and their demographic data.</p><h3>Results</h3><p dir="ltr">Genomic DNA from 54 isolates were tested by PCR and sequence analysis at three loci: glutamate dehydrogenase (gdh), β-giardin (bg) and triose phosphate (tpi)). Assemblage A was identified in nine (16.67%), assemblage B in thirty (55.55%), and a mixture of assemblages A+B in fifteen (27.78%) isolates. All assemblage A isolates, genotyped in different loci, were assigned to sub-assemblage AII, and six of them had MLGs AII-1 while one new MLG was identified in two isolates. Sequences of assemblage B isolates have high level of genetic diversity and high presence of heterogeneous peaks, especially within the gdh gene. No significant associations between genotypes and the immigrants’ demographic data were found due to the extensive number of new variants.</p><h3>Conclusions</h3><p dir="ltr">MLGs was used herein to genotype 54 immigrant Giardia isolates. The high level of genetic variability found in our isolates hampered MLGs determination, more investigations are now required to consolidate our findings, and to enable a comprehensive understanding of the diversity within G. duodenalis assemblage B isolates.</p><h2>Other Information</h2><p dir="ltr">Published in: Frontiers in Cellular and Infection Microbiology<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3389/fcimb.2021.652946" target="_blank">https://dx.doi.org/10.3389/fcimb.2021.652946</a></p><p dir="ltr">Additional institutions affiliated with: Medical Commission - MOPH</p>2021-05-04T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3389/fcimb.2021.652946https://figshare.com/articles/journal_contribution/Genetic_Diversity_and_Prevalence_of_Giardia_duodenalis_in_Qatar/26020900CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/260209002021-05-04T03:00:00Z |
| spellingShingle | Genetic Diversity and Prevalence of Giardia duodenalis in Qatar Marwa Chourabi (10742520) Biomedical and clinical sciences Medical microbiology Health sciences Epidemiology G. duodenalis assemblages multilocus genotyping Qatar immigrant |
| status_str | publishedVersion |
| title | Genetic Diversity and Prevalence of Giardia duodenalis in Qatar |
| title_full | Genetic Diversity and Prevalence of Giardia duodenalis in Qatar |
| title_fullStr | Genetic Diversity and Prevalence of Giardia duodenalis in Qatar |
| title_full_unstemmed | Genetic Diversity and Prevalence of Giardia duodenalis in Qatar |
| title_short | Genetic Diversity and Prevalence of Giardia duodenalis in Qatar |
| title_sort | Genetic Diversity and Prevalence of Giardia duodenalis in Qatar |
| topic | Biomedical and clinical sciences Medical microbiology Health sciences Epidemiology G. duodenalis assemblages multilocus genotyping Qatar immigrant |