Targeted Inactivation of Rin3 Increases Trabecular Bone Mass by Reducing Bone Resorption and Favouring Bone Formation
<p dir="ltr">Common genetic variants at the RIN3 locus on chromosome 14q32 predispose to Paget’s disease of bone (PDB) but the mechanisms by which they do so are unknown. Here, we analysed the skeletal phenotype of female mice with targeted inactivation of the mouse Rin3 gene (Rin3&l...
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2021
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| _version_ | 1864513517566033920 |
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| author | Mahéva Vallet (18456729) |
| author2 | Antonia Sophocleous (7926470) Anna E. Törnqvist (14106644) Asim Azfer (12809970) Rob van’t Hof (10526383) Omar ME Albagha (18456732) Stuart H. Ralston (311778) |
| author2_role | author author author author author author |
| author_facet | Mahéva Vallet (18456729) Antonia Sophocleous (7926470) Anna E. Törnqvist (14106644) Asim Azfer (12809970) Rob van’t Hof (10526383) Omar ME Albagha (18456732) Stuart H. Ralston (311778) |
| author_role | author |
| dc.creator.none.fl_str_mv | Mahéva Vallet (18456729) Antonia Sophocleous (7926470) Anna E. Törnqvist (14106644) Asim Azfer (12809970) Rob van’t Hof (10526383) Omar ME Albagha (18456732) Stuart H. Ralston (311778) |
| dc.date.none.fl_str_mv | 2021-03-16T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1007/s00223-021-00827-2 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Targeted_Inactivation_of_Rin3_Increases_Trabecular_Bone_Mass_by_Reducing_Bone_Resorption_and_Favouring_Bone_Formation/25712148 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Genetics Paget’s disease Osteoclast Genetic RIN3 |
| dc.title.none.fl_str_mv | Targeted Inactivation of Rin3 Increases Trabecular Bone Mass by Reducing Bone Resorption and Favouring Bone Formation |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Common genetic variants at the RIN3 locus on chromosome 14q32 predispose to Paget’s disease of bone (PDB) but the mechanisms by which they do so are unknown. Here, we analysed the skeletal phenotype of female mice with targeted inactivation of the mouse Rin3 gene (Rin3<sup>−</sup>/<sup>−</sup>) as compared with wild-type littermates. The Rin3<sup>−</sup>/<sup>− </sup>mice had higher trabecular bone volume (BV/TV%) compared with wild type. Mean ± standard deviation values at the distal femur at 8 weeks were 9.0 ± 2.5 vs. 7.0 ± 1.5 (p = 0.002) and at 52 weeks were 15.8 ± 9.5 vs. 8.5 ± 4.2 (p = 0.002). No differences were observed in femoral cortical bone parameters with the exception of marrow diameter which was significantly smaller in 52-week-old Rin3<sup>−</sup>/<sup>−</sup> mice compared to wild type: (0.43 mm ± 0.1 vs. 0.57 mm ± 0.2 (p = 0.001). Bone histomorphometry showed a lower osteoclast surface / bone surface (Oc.S/BS%) at 8 weeks in Rin3<sup>−</sup>/<sup>−</sup> mice compared to wild type (24.1 ± 4.7 vs. 29.7 ± 6.6; p = 0.025) but there were no significant differences in markers of bone formation at this time. At 52 weeks, Oc.S/BS did not differ between genotypes but single labelled perimeter (SL.Pm/B.Pm (%)) was significantly higher in Rin3<sup>−</sup>/<sup>−</sup> mice (24.4 ± 6.4 vs. 16.5 ± 3.8, p = 0.003). We conclude that Rin3 negatively regulates trabecular bone mass in mice by inhibiting osteoclastic bone resorption and favouring bone formation. Our observations also suggest that the variants that predispose to PDB in humans probably do so by causing a gain-in-function of RIN3.</p><p dir="ltr">Correction: Targeted Inactivation of <i>Rin3</i> Increases Trabecular Bone Mass by Reducing Bone Resorption and Favouring Bone Formation <a href="https://doi.org/10.1007/s00223-021-00849-w" target="_blank">https://doi.org/10.1007/s00223-021-00849-w</a>, published online 19 April 2021.</p><h2>Other Information</h2><p dir="ltr">Published in: Calcified Tissue International<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1007/s00223-021-00827-2" target="_blank">https://dx.doi.org/10.1007/s00223-021-00827-2</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_6eb14c844d94a94c939aac2ae4411d55 |
| identifier_str_mv | 10.1007/s00223-021-00827-2 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25712148 |
| publishDate | 2021 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Targeted Inactivation of Rin3 Increases Trabecular Bone Mass by Reducing Bone Resorption and Favouring Bone FormationMahéva Vallet (18456729)Antonia Sophocleous (7926470)Anna E. Törnqvist (14106644)Asim Azfer (12809970)Rob van’t Hof (10526383)Omar ME Albagha (18456732)Stuart H. Ralston (311778)Biological sciencesGeneticsPaget’s diseaseOsteoclastGeneticRIN3<p dir="ltr">Common genetic variants at the RIN3 locus on chromosome 14q32 predispose to Paget’s disease of bone (PDB) but the mechanisms by which they do so are unknown. Here, we analysed the skeletal phenotype of female mice with targeted inactivation of the mouse Rin3 gene (Rin3<sup>−</sup>/<sup>−</sup>) as compared with wild-type littermates. The Rin3<sup>−</sup>/<sup>− </sup>mice had higher trabecular bone volume (BV/TV%) compared with wild type. Mean ± standard deviation values at the distal femur at 8 weeks were 9.0 ± 2.5 vs. 7.0 ± 1.5 (p = 0.002) and at 52 weeks were 15.8 ± 9.5 vs. 8.5 ± 4.2 (p = 0.002). No differences were observed in femoral cortical bone parameters with the exception of marrow diameter which was significantly smaller in 52-week-old Rin3<sup>−</sup>/<sup>−</sup> mice compared to wild type: (0.43 mm ± 0.1 vs. 0.57 mm ± 0.2 (p = 0.001). Bone histomorphometry showed a lower osteoclast surface / bone surface (Oc.S/BS%) at 8 weeks in Rin3<sup>−</sup>/<sup>−</sup> mice compared to wild type (24.1 ± 4.7 vs. 29.7 ± 6.6; p = 0.025) but there were no significant differences in markers of bone formation at this time. At 52 weeks, Oc.S/BS did not differ between genotypes but single labelled perimeter (SL.Pm/B.Pm (%)) was significantly higher in Rin3<sup>−</sup>/<sup>−</sup> mice (24.4 ± 6.4 vs. 16.5 ± 3.8, p = 0.003). We conclude that Rin3 negatively regulates trabecular bone mass in mice by inhibiting osteoclastic bone resorption and favouring bone formation. Our observations also suggest that the variants that predispose to PDB in humans probably do so by causing a gain-in-function of RIN3.</p><p dir="ltr">Correction: Targeted Inactivation of <i>Rin3</i> Increases Trabecular Bone Mass by Reducing Bone Resorption and Favouring Bone Formation <a href="https://doi.org/10.1007/s00223-021-00849-w" target="_blank">https://doi.org/10.1007/s00223-021-00849-w</a>, published online 19 April 2021.</p><h2>Other Information</h2><p dir="ltr">Published in: Calcified Tissue International<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1007/s00223-021-00827-2" target="_blank">https://dx.doi.org/10.1007/s00223-021-00827-2</a></p>2021-03-16T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1007/s00223-021-00827-2https://figshare.com/articles/journal_contribution/Targeted_Inactivation_of_Rin3_Increases_Trabecular_Bone_Mass_by_Reducing_Bone_Resorption_and_Favouring_Bone_Formation/25712148CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/257121482021-03-16T03:00:00Z |
| spellingShingle | Targeted Inactivation of Rin3 Increases Trabecular Bone Mass by Reducing Bone Resorption and Favouring Bone Formation Mahéva Vallet (18456729) Biological sciences Genetics Paget’s disease Osteoclast Genetic RIN3 |
| status_str | publishedVersion |
| title | Targeted Inactivation of Rin3 Increases Trabecular Bone Mass by Reducing Bone Resorption and Favouring Bone Formation |
| title_full | Targeted Inactivation of Rin3 Increases Trabecular Bone Mass by Reducing Bone Resorption and Favouring Bone Formation |
| title_fullStr | Targeted Inactivation of Rin3 Increases Trabecular Bone Mass by Reducing Bone Resorption and Favouring Bone Formation |
| title_full_unstemmed | Targeted Inactivation of Rin3 Increases Trabecular Bone Mass by Reducing Bone Resorption and Favouring Bone Formation |
| title_short | Targeted Inactivation of Rin3 Increases Trabecular Bone Mass by Reducing Bone Resorption and Favouring Bone Formation |
| title_sort | Targeted Inactivation of Rin3 Increases Trabecular Bone Mass by Reducing Bone Resorption and Favouring Bone Formation |
| topic | Biological sciences Genetics Paget’s disease Osteoclast Genetic RIN3 |