Genetic analysis of QTLs controlling allelopathic characteristics in sorghum
<p dir="ltr">Mature sorghum herbage is known to contain several water-soluble secondary metabolites (allelochemicals). In this study, we investigated quantitative trait loci (QTLs) associated with allelochemical characteristics in sorghum using linkage mapping and linkage disequilibr...
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| المؤلف الرئيسي: | |
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| مؤلفون آخرون: | |
| منشور في: |
2020
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| الموضوعات: | |
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إضافة وسم
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| _version_ | 1864513554006147072 |
|---|---|
| author | Tariq Shehzad (6195398) |
| author2 | Kazutoshi Okuno (9188056) |
| author2_role | author |
| author_facet | Tariq Shehzad (6195398) Kazutoshi Okuno (9188056) |
| author_role | author |
| dc.creator.none.fl_str_mv | Tariq Shehzad (6195398) Kazutoshi Okuno (9188056) |
| dc.date.none.fl_str_mv | 2020-07-30T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1371/journal.pone.0235896 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Genetic_analysis_of_QTLs_controlling_allelopathic_characteristics_in_sorghum/27935229 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Genetics Quantitative trait loci Sorghum Lettuce Linkage mapping Seed germination Seeds Genetic loci Weeds |
| dc.title.none.fl_str_mv | Genetic analysis of QTLs controlling allelopathic characteristics in sorghum |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Mature sorghum herbage is known to contain several water-soluble secondary metabolites (allelochemicals). In this study, we investigated quantitative trait loci (QTLs) associated with allelochemical characteristics in sorghum using linkage mapping and linkage disequilibrium (LD)-based association mapping. A sorghum diversity research set (SDRS) of 107 accessions was used in LD mapping whereas, F<sub>2:3</sub> lines derived from a cross between Japanese and African landraces were used in linkage mapping. The QTLs were further confirmed by positional (targeted) association mapping with Q+K model. The inhibitory effect of water-soluble extracts (WSE) was tested on germination and root length of lettuce seedlings in four concentrations (25%, 50%, 75% and 100%). A Significant range of variations was observed among genotypes in both types of mapping populations (P < 0.05). A total of 181 simple sequence repeats (SSRs) derived from antecedently reported map have been used for genotyping of SDRS. A genetic linkage map of 151 sorghum SSR markers was also developed on 134 F<sub>2</sub> individuals. The total map length was 1359.3 cM, with an average distance of 8.2 cM between adjacent markers. LD mapping identified three QTLs for inhibition effect on germination and seven QTLs for root length of lettuce seedlings. Whereas, a total of six QTLs for inhibition of germination and ten QTLs for root length were detected in linkage mapping approach. The percent phenotypic variation explained by individual QTL ranged from 6.9% to 27.3% in SDRS and 9.9% to 35.6% in F<sub>2:3</sub> lines. Regional association analysis identified four QTLs, three of them are common in other methods too. No QTL was identified in the region where major gene for sorgoleone (SOR1) has been cloned previously on chromosome 5.</p><h2>Other Information</h2><p dir="ltr">Published in: PLOS ONE<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1371/journal.pone.0235896" target="_blank">https://dx.doi.org/10.1371/journal.pone.0235896</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_756d0f8f723ee67c3b9cd8f43167fa04 |
| identifier_str_mv | 10.1371/journal.pone.0235896 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/27935229 |
| publishDate | 2020 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Genetic analysis of QTLs controlling allelopathic characteristics in sorghumTariq Shehzad (6195398)Kazutoshi Okuno (9188056)Biological sciencesGeneticsQuantitative trait lociSorghumLettuceLinkage mappingSeed germinationSeedsGenetic lociWeeds<p dir="ltr">Mature sorghum herbage is known to contain several water-soluble secondary metabolites (allelochemicals). In this study, we investigated quantitative trait loci (QTLs) associated with allelochemical characteristics in sorghum using linkage mapping and linkage disequilibrium (LD)-based association mapping. A sorghum diversity research set (SDRS) of 107 accessions was used in LD mapping whereas, F<sub>2:3</sub> lines derived from a cross between Japanese and African landraces were used in linkage mapping. The QTLs were further confirmed by positional (targeted) association mapping with Q+K model. The inhibitory effect of water-soluble extracts (WSE) was tested on germination and root length of lettuce seedlings in four concentrations (25%, 50%, 75% and 100%). A Significant range of variations was observed among genotypes in both types of mapping populations (P < 0.05). A total of 181 simple sequence repeats (SSRs) derived from antecedently reported map have been used for genotyping of SDRS. A genetic linkage map of 151 sorghum SSR markers was also developed on 134 F<sub>2</sub> individuals. The total map length was 1359.3 cM, with an average distance of 8.2 cM between adjacent markers. LD mapping identified three QTLs for inhibition effect on germination and seven QTLs for root length of lettuce seedlings. Whereas, a total of six QTLs for inhibition of germination and ten QTLs for root length were detected in linkage mapping approach. The percent phenotypic variation explained by individual QTL ranged from 6.9% to 27.3% in SDRS and 9.9% to 35.6% in F<sub>2:3</sub> lines. Regional association analysis identified four QTLs, three of them are common in other methods too. No QTL was identified in the region where major gene for sorgoleone (SOR1) has been cloned previously on chromosome 5.</p><h2>Other Information</h2><p dir="ltr">Published in: PLOS ONE<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1371/journal.pone.0235896" target="_blank">https://dx.doi.org/10.1371/journal.pone.0235896</a></p>2020-07-30T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1371/journal.pone.0235896https://figshare.com/articles/journal_contribution/Genetic_analysis_of_QTLs_controlling_allelopathic_characteristics_in_sorghum/27935229CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/279352292020-07-30T03:00:00Z |
| spellingShingle | Genetic analysis of QTLs controlling allelopathic characteristics in sorghum Tariq Shehzad (6195398) Biological sciences Genetics Quantitative trait loci Sorghum Lettuce Linkage mapping Seed germination Seeds Genetic loci Weeds |
| status_str | publishedVersion |
| title | Genetic analysis of QTLs controlling allelopathic characteristics in sorghum |
| title_full | Genetic analysis of QTLs controlling allelopathic characteristics in sorghum |
| title_fullStr | Genetic analysis of QTLs controlling allelopathic characteristics in sorghum |
| title_full_unstemmed | Genetic analysis of QTLs controlling allelopathic characteristics in sorghum |
| title_short | Genetic analysis of QTLs controlling allelopathic characteristics in sorghum |
| title_sort | Genetic analysis of QTLs controlling allelopathic characteristics in sorghum |
| topic | Biological sciences Genetics Quantitative trait loci Sorghum Lettuce Linkage mapping Seed germination Seeds Genetic loci Weeds |