The Replacement of five Consecutive Amino Acids in the Cyt1A Protein of Bacillus thuringiensis Enhances its Cytotoxic Activity against Lung Epithelial Cancer Cells
<p dir="ltr">Cyt1A protein is a cytolytic protein encoded by the <i>cyt</i> gene of <i>Bacillus thuringiensis</i> subsp. <i>israelensis</i> (Bti) as part of the parasporal crystal proteins produced during the sporulation. Cyt1A protein is unique co...
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2018
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| _version_ | 1864513526392946688 |
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| author | Kavita Nair (17151127) |
| author2 | Ahmad Iskandarani (3520067) Roda Al-Thani (540907) Ramzi Mohammad (3520061) Samir Jaoua (11624805) |
| author2_role | author author author author |
| author_facet | Kavita Nair (17151127) Ahmad Iskandarani (3520067) Roda Al-Thani (540907) Ramzi Mohammad (3520061) Samir Jaoua (11624805) |
| author_role | author |
| dc.creator.none.fl_str_mv | Kavita Nair (17151127) Ahmad Iskandarani (3520067) Roda Al-Thani (540907) Ramzi Mohammad (3520061) Samir Jaoua (11624805) |
| dc.date.none.fl_str_mv | 2018-03-16T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3390/toxins10030125 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/The_Replacement_of_five_Consecutive_Amino_Acids_in_the_Cyt1A_Protein_of_Bacillus_thuringiensis_Enhances_its_Cytotoxic_Activity_against_Lung_Epithelial_Cancer_Cells/25294888 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Pharmacology and pharmaceutical sciences Bacillus thuringiensis subsp. israelensis Novel Cyt1A cytotoxicity lung epithelial cancer cell line protein modelling |
| dc.title.none.fl_str_mv | The Replacement of five Consecutive Amino Acids in the Cyt1A Protein of Bacillus thuringiensis Enhances its Cytotoxic Activity against Lung Epithelial Cancer Cells |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Cyt1A protein is a cytolytic protein encoded by the <i>cyt</i> gene of <i>Bacillus thuringiensis</i> subsp. <i>israelensis</i> (Bti) as part of the parasporal crystal proteins produced during the sporulation. Cyt1A protein is unique compared to the other endotoxins present in these parasporal crystals. Unlike δ-endotoxins, Cyt1A protein does not require receptors to bind to the target cell and activate the toxicity. It has the ability to affect a broad range of cell types and organisms, due to this characteristic. Cyt1A has been recognized to not only target the insect cells directly, but also recruit other endotoxins by acting as receptors. Due to these mode of actions, Cyt1A has been studied for its cytolytic activity against human cancer cell lines, although not extensively. In this study, we report a novel Cyt1A protein produced by a Bti strain QBT229 isolated from Qatar. When tested for its cytotoxicity against lung cancer cells, this local strain showed considerably higher activity compared to that of the reference Bti and other strains tested. The possible reasons for such enhanced activity were explored at the gene and protein levels. It was evidenced that five consecutive amino acid replacements in the β8 sheet of the Cyt1A protein enhanced the cytotoxicity against the lung epithelial cancer cells. Such novel Cyt1A protein with high cytotoxicity against lung cancer cells has been characterized and reported through this study.</p><h2>Other Information</h2><p dir="ltr">Published in: Toxins<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/toxins10030125" target="_blank">https://dx.doi.org/10.3390/toxins10030125</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_76e5f61a7a08b789a4e67c90da96f9a1 |
| identifier_str_mv | 10.3390/toxins10030125 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25294888 |
| publishDate | 2018 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | The Replacement of five Consecutive Amino Acids in the Cyt1A Protein of Bacillus thuringiensis Enhances its Cytotoxic Activity against Lung Epithelial Cancer CellsKavita Nair (17151127)Ahmad Iskandarani (3520067)Roda Al-Thani (540907)Ramzi Mohammad (3520061)Samir Jaoua (11624805)Biomedical and clinical sciencesPharmacology and pharmaceutical sciencesBacillus thuringiensis subsp. israelensisNovel Cyt1Acytotoxicitylung epithelial cancer cell lineprotein modelling<p dir="ltr">Cyt1A protein is a cytolytic protein encoded by the <i>cyt</i> gene of <i>Bacillus thuringiensis</i> subsp. <i>israelensis</i> (Bti) as part of the parasporal crystal proteins produced during the sporulation. Cyt1A protein is unique compared to the other endotoxins present in these parasporal crystals. Unlike δ-endotoxins, Cyt1A protein does not require receptors to bind to the target cell and activate the toxicity. It has the ability to affect a broad range of cell types and organisms, due to this characteristic. Cyt1A has been recognized to not only target the insect cells directly, but also recruit other endotoxins by acting as receptors. Due to these mode of actions, Cyt1A has been studied for its cytolytic activity against human cancer cell lines, although not extensively. In this study, we report a novel Cyt1A protein produced by a Bti strain QBT229 isolated from Qatar. When tested for its cytotoxicity against lung cancer cells, this local strain showed considerably higher activity compared to that of the reference Bti and other strains tested. The possible reasons for such enhanced activity were explored at the gene and protein levels. It was evidenced that five consecutive amino acid replacements in the β8 sheet of the Cyt1A protein enhanced the cytotoxicity against the lung epithelial cancer cells. Such novel Cyt1A protein with high cytotoxicity against lung cancer cells has been characterized and reported through this study.</p><h2>Other Information</h2><p dir="ltr">Published in: Toxins<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/toxins10030125" target="_blank">https://dx.doi.org/10.3390/toxins10030125</a></p>2018-03-16T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3390/toxins10030125https://figshare.com/articles/journal_contribution/The_Replacement_of_five_Consecutive_Amino_Acids_in_the_Cyt1A_Protein_of_Bacillus_thuringiensis_Enhances_its_Cytotoxic_Activity_against_Lung_Epithelial_Cancer_Cells/25294888CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/252948882018-03-16T03:00:00Z |
| spellingShingle | The Replacement of five Consecutive Amino Acids in the Cyt1A Protein of Bacillus thuringiensis Enhances its Cytotoxic Activity against Lung Epithelial Cancer Cells Kavita Nair (17151127) Biomedical and clinical sciences Pharmacology and pharmaceutical sciences Bacillus thuringiensis subsp. israelensis Novel Cyt1A cytotoxicity lung epithelial cancer cell line protein modelling |
| status_str | publishedVersion |
| title | The Replacement of five Consecutive Amino Acids in the Cyt1A Protein of Bacillus thuringiensis Enhances its Cytotoxic Activity against Lung Epithelial Cancer Cells |
| title_full | The Replacement of five Consecutive Amino Acids in the Cyt1A Protein of Bacillus thuringiensis Enhances its Cytotoxic Activity against Lung Epithelial Cancer Cells |
| title_fullStr | The Replacement of five Consecutive Amino Acids in the Cyt1A Protein of Bacillus thuringiensis Enhances its Cytotoxic Activity against Lung Epithelial Cancer Cells |
| title_full_unstemmed | The Replacement of five Consecutive Amino Acids in the Cyt1A Protein of Bacillus thuringiensis Enhances its Cytotoxic Activity against Lung Epithelial Cancer Cells |
| title_short | The Replacement of five Consecutive Amino Acids in the Cyt1A Protein of Bacillus thuringiensis Enhances its Cytotoxic Activity against Lung Epithelial Cancer Cells |
| title_sort | The Replacement of five Consecutive Amino Acids in the Cyt1A Protein of Bacillus thuringiensis Enhances its Cytotoxic Activity against Lung Epithelial Cancer Cells |
| topic | Biomedical and clinical sciences Pharmacology and pharmaceutical sciences Bacillus thuringiensis subsp. israelensis Novel Cyt1A cytotoxicity lung epithelial cancer cell line protein modelling |