A calcium peroxide incorporated oxygen releasing chitosan-PVA patch for Diabetic wound healing

<p dir="ltr">Impaired wound healing is a major healthcare problem in patients with diabetes often resulting in gangrene, microbial infection and amputation of affected limb. The delay or absence in healing process arises from several abnormalities, among them chronic hypoxia is a maj...

وصف كامل

محفوظ في:
التفاصيل البيبلوغرافية
المؤلف الرئيسي: Asad Ullah (4524541) (author)
مؤلفون آخرون: Abdulla Al Mamun (8773013) (author), Midhat Batool Zaidi (18024226) (author), Talat Roome (4806630) (author), Anwarul Hasan (1332066) (author)
منشور في: 2023
الموضوعات:
الوسوم: إضافة وسم
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author Asad Ullah (4524541)
author2 Abdulla Al Mamun (8773013)
Midhat Batool Zaidi (18024226)
Talat Roome (4806630)
Anwarul Hasan (1332066)
author2_role author
author
author
author
author_facet Asad Ullah (4524541)
Abdulla Al Mamun (8773013)
Midhat Batool Zaidi (18024226)
Talat Roome (4806630)
Anwarul Hasan (1332066)
author_role author
dc.creator.none.fl_str_mv Asad Ullah (4524541)
Abdulla Al Mamun (8773013)
Midhat Batool Zaidi (18024226)
Talat Roome (4806630)
Anwarul Hasan (1332066)
dc.date.none.fl_str_mv 2023-09-01T00:00:00Z
dc.identifier.none.fl_str_mv 10.1016/j.biopha.2023.115156
dc.relation.none.fl_str_mv https://figshare.com/articles/journal_contribution/A_calcium_peroxide_incorporated_oxygen_releasing_chitosan-PVA_patch_for_Diabetic_wound_healing/25287385
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Biomedical and clinical sciences
Medical biochemistry and metabolomics
Pharmacology and pharmaceutical sciences
Engineering
Biomedical engineering
Materials engineering
Nanotechnology
Diabetic wound healing
Chronic hypoxia
Oxygen releasing materials
dc.title.none.fl_str_mv A calcium peroxide incorporated oxygen releasing chitosan-PVA patch for Diabetic wound healing
dc.type.none.fl_str_mv Text
Journal contribution
info:eu-repo/semantics/publishedVersion
text
contribution to journal
description <p dir="ltr">Impaired wound healing is a major healthcare problem in patients with diabetes often resulting in gangrene, microbial infection and amputation of affected limb. The delay or absence in healing process arises from several abnormalities, among them chronic hypoxia is a major concern due to its associated issues such as lack of collagen deposition, epithelization, fibroplasia, angiogenesis, and resistance to infections at the wound site. To address hypoxia, delivery of oxygen at the wound site through oxygen releasing agents have been proven to be effective therapeutics. Several oxygen releasing nanoparticles such as Sodium Percarbonate (SPC), Calcium Peroxide (CPO), Hydrogen Peroxide, Magnesium Peroxide (MPO) have been investigated in wound healing application. However, the uncontrolled/burst release of these nanotherapeutic agents and its accompanied cytotoxicity pose a barrier in expediting the healing process. In this study, a Chitosan-Polyvinyl alcohol (CS-PVA) based hydrogel containing oxygen releasing nanoparticle, calcium peroxide (CPO) was constructed to provide a slow and sustained delivery of oxygen for at least 5 days. In-vitro cell culture studies with this material using fibroblast and endothelial cell line exhibited improved biocompatibility, cell viability and enhanced proliferation in comparison with the control group. Additionally, cell migration study using scratch assay method showed superior cell migration ability of our proposed materials. Furthermore, In vivo study using diabetic rat model showed accelerated wound closure rate compared to untreated control wounds.</p><h2>Other Information</h2><p dir="ltr">Published in: Biomedicine & Pharmacotherapy<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.biopha.2023.115156" rel="noreferrer" target="_blank">https://dx.doi.org/10.1016/j.biopha.2023.115156</a><br>Additional institutions affiliated with: Pak-Qatar Family Takaful Limited (PQFTL), Dow University of Health Sciences Qatar and Dow International Medical College – DUHSQ</p>
eu_rights_str_mv openAccess
id Manara2_77e316523f6ad65236b3c0dbeddd024a
identifier_str_mv 10.1016/j.biopha.2023.115156
network_acronym_str Manara2
network_name_str Manara2
oai_identifier_str oai:figshare.com:article/25287385
publishDate 2023
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rights_invalid_str_mv CC BY 4.0
spelling A calcium peroxide incorporated oxygen releasing chitosan-PVA patch for Diabetic wound healingAsad Ullah (4524541)Abdulla Al Mamun (8773013)Midhat Batool Zaidi (18024226)Talat Roome (4806630)Anwarul Hasan (1332066)Biomedical and clinical sciencesMedical biochemistry and metabolomicsPharmacology and pharmaceutical sciencesEngineeringBiomedical engineeringMaterials engineeringNanotechnologyDiabetic wound healingChronic hypoxiaOxygen releasing materials<p dir="ltr">Impaired wound healing is a major healthcare problem in patients with diabetes often resulting in gangrene, microbial infection and amputation of affected limb. The delay or absence in healing process arises from several abnormalities, among them chronic hypoxia is a major concern due to its associated issues such as lack of collagen deposition, epithelization, fibroplasia, angiogenesis, and resistance to infections at the wound site. To address hypoxia, delivery of oxygen at the wound site through oxygen releasing agents have been proven to be effective therapeutics. Several oxygen releasing nanoparticles such as Sodium Percarbonate (SPC), Calcium Peroxide (CPO), Hydrogen Peroxide, Magnesium Peroxide (MPO) have been investigated in wound healing application. However, the uncontrolled/burst release of these nanotherapeutic agents and its accompanied cytotoxicity pose a barrier in expediting the healing process. In this study, a Chitosan-Polyvinyl alcohol (CS-PVA) based hydrogel containing oxygen releasing nanoparticle, calcium peroxide (CPO) was constructed to provide a slow and sustained delivery of oxygen for at least 5 days. In-vitro cell culture studies with this material using fibroblast and endothelial cell line exhibited improved biocompatibility, cell viability and enhanced proliferation in comparison with the control group. Additionally, cell migration study using scratch assay method showed superior cell migration ability of our proposed materials. Furthermore, In vivo study using diabetic rat model showed accelerated wound closure rate compared to untreated control wounds.</p><h2>Other Information</h2><p dir="ltr">Published in: Biomedicine & Pharmacotherapy<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1016/j.biopha.2023.115156" rel="noreferrer" target="_blank">https://dx.doi.org/10.1016/j.biopha.2023.115156</a><br>Additional institutions affiliated with: Pak-Qatar Family Takaful Limited (PQFTL), Dow University of Health Sciences Qatar and Dow International Medical College – DUHSQ</p>2023-09-01T00:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1016/j.biopha.2023.115156https://figshare.com/articles/journal_contribution/A_calcium_peroxide_incorporated_oxygen_releasing_chitosan-PVA_patch_for_Diabetic_wound_healing/25287385CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/252873852023-09-01T00:00:00Z
spellingShingle A calcium peroxide incorporated oxygen releasing chitosan-PVA patch for Diabetic wound healing
Asad Ullah (4524541)
Biomedical and clinical sciences
Medical biochemistry and metabolomics
Pharmacology and pharmaceutical sciences
Engineering
Biomedical engineering
Materials engineering
Nanotechnology
Diabetic wound healing
Chronic hypoxia
Oxygen releasing materials
status_str publishedVersion
title A calcium peroxide incorporated oxygen releasing chitosan-PVA patch for Diabetic wound healing
title_full A calcium peroxide incorporated oxygen releasing chitosan-PVA patch for Diabetic wound healing
title_fullStr A calcium peroxide incorporated oxygen releasing chitosan-PVA patch for Diabetic wound healing
title_full_unstemmed A calcium peroxide incorporated oxygen releasing chitosan-PVA patch for Diabetic wound healing
title_short A calcium peroxide incorporated oxygen releasing chitosan-PVA patch for Diabetic wound healing
title_sort A calcium peroxide incorporated oxygen releasing chitosan-PVA patch for Diabetic wound healing
topic Biomedical and clinical sciences
Medical biochemistry and metabolomics
Pharmacology and pharmaceutical sciences
Engineering
Biomedical engineering
Materials engineering
Nanotechnology
Diabetic wound healing
Chronic hypoxia
Oxygen releasing materials