Downregulation of Lymphoid enhancer‐binding factor 1 (<i>LEF‐1)</i> expression (by immunohistochemistry and/ flow cytometry) in chronic Lymphocytic Leukemia with atypical immunophenotypic and cytologic features
<h3>Introduction</h3><p dir="ltr">Lymphoid enhancer-binding factor 1 (<i>LEF-1</i>) overexpression has been recently remarkably reported in chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) and has shown utility in distinguishing CLL/SLL from o...
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2020
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| Summary: | <h3>Introduction</h3><p dir="ltr">Lymphoid enhancer-binding factor 1 (<i>LEF-1</i>) overexpression has been recently remarkably reported in chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) and has shown utility in distinguishing CLL/SLL from other B-cell lymphomas. CLL has a well-defined immunophenotype, yet, some cases of CLL demonstrate atypical morphology/ phenotype reflected by low Matutes score (atypical CLL). Till date, <i>LEF1</i> expression has not been systematically studied in cases of CLL with atypical features.</p><h3>Methods</h3><p dir="ltr">In this study, <i>LEF-1</i> expression was assessed by two different techniques, (immunohistochemistry and flow cytometry), to investigate the expression profile of <i>LEF-1</i> in cases of CLL/SLL, in comparison with other low-grade B-lymphomas and CLL with atypical features, including atypical immunophenotype and CLL with increased prolymphocytes or morphologically atypical cells.</p><h3>Results</h3><p dir="ltr">We found that <i>LEF-1</i> expression is downregulated in CLL with atypical immunophenotype/features compared to classic CLL; Chi-Square <i>P</i> < .0001. The ratio for <i>LEF-1</i> expression in malignant B-cells/NK (by flow cytometry) in CLL/SLL with classic immunophenotype was higher than atypical CLL and is significantly higher in other small B-cell lymphomas (<i>P </i>< .01). Absence of <i>LEF-1</i> expression in CLL/SLL is correlated (<i>P</i> < .05) with downregulation of CD5, CD23, CD200, expression of FMC7, brighter expression of CD79b, brighter expression of surface light chain, increased prolymphocytes and lower Matutes score.</p><h3>Conclusion</h3><p dir="ltr">As downregulation of <i>LEF-1</i> expression is well correlated with atypical CLL, we suggest adding <i>LEF-1</i> to Matutes score as a beneficial marker to differentiate classic from atypical CLL <i>LEF-1</i> could also serve as a potential prognostic indicator for CLL clinical course.</p><h2>Other Information</h2><p dir="ltr">Published in: International Journal of Laboratory Hematology<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="http://dx.doi.org/10.1111/ijlh.13420" target="_blank">http://dx.doi.org/10.1111/ijlh.13420</a></p> |
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