Determination of Medicago orbicularis Antioxidant, Antihemolytic, and Anti-Cancerous Activities and Its Augmentation of Cisplatin-Induced Cytotoxicity in A549 Lung Cancer Cells
<p dir="ltr">The anti-lung cancer properties of the plant <i>Medicago orbicularis</i> have not been explored yet. Therefore, we identified its phytochemical composition and investigated the antioxidant, anti-hemolytic, and anti-cancerous properties of extracts of this pla...
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| مؤلفون آخرون: | , , , , , |
| منشور في: |
2024
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| _version_ | 1864513534563450880 |
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| author | Abdullah Shaito (20545181) |
| author2 | Islam Omairi (22481697) Najlaa A. Al Thani (17788493) Fatiha Seglab (22468265) Esraa Ad-Darwish (22481703) Firas Kobeissy (17849094) Salam Nasreddine (214796) |
| author2_role | author author author author author author |
| author_facet | Abdullah Shaito (20545181) Islam Omairi (22481697) Najlaa A. Al Thani (17788493) Fatiha Seglab (22468265) Esraa Ad-Darwish (22481703) Firas Kobeissy (17849094) Salam Nasreddine (214796) |
| author_role | author |
| dc.creator.none.fl_str_mv | Abdullah Shaito (20545181) Islam Omairi (22481697) Najlaa A. Al Thani (17788493) Fatiha Seglab (22468265) Esraa Ad-Darwish (22481703) Firas Kobeissy (17849094) Salam Nasreddine (214796) |
| dc.date.none.fl_str_mv | 2024-02-02T06:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3390/plants13030442 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Determination_of_Medicago_orbicularis_Antioxidant_Antihemolytic_and_Anti-Cancerous_Activities_and_Its_Augmentation_of_Cisplatin-Induced_Cytotoxicity_in_A549_Lung_Cancer_Cells/30415072 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Agricultural, veterinary and food sciences Crop and pasture production Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Cardiovascular medicine and haematology Oncology and carcinogenesis Pharmacology and pharmaceutical sciences Health sciences Traditional, complementary and integrative medicine Medicago orbicularis lung cancer herbal medicine antioxidants hemolytic cisplatin A549 cells |
| dc.title.none.fl_str_mv | Determination of Medicago orbicularis Antioxidant, Antihemolytic, and Anti-Cancerous Activities and Its Augmentation of Cisplatin-Induced Cytotoxicity in A549 Lung Cancer Cells |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">The anti-lung cancer properties of the plant <i>Medicago orbicularis</i> have not been explored yet. Therefore, we identified its phytochemical composition and investigated the antioxidant, anti-hemolytic, and anti-cancerous properties of extracts of this plant in A549 human lung adenocarcinoma cells. The results show that all parts of <i>M. orbicularis</i> (stems, leaves, and fruits) exhibit remarkable hemolytic activities and modest antioxidant capacity. In addition, all extracts showed a dose-dependent anti-cancerous cytotoxic activity against A549 cells, with fruit extracts being the most potent. This cytotoxic effect could be related, at least partly, to the induction of apoptosis, where <i>M. orbicularis</i> fruit extracts reduced the ratio of anti-apoptotic BCL-2/pro-apoptotic BAX, thereby promoting cellular death. Furthermore, the use of <i>M. orbicularis</i>, in combination with a conventional chemotherapeutic agent, cisplatin, was assessed. Indeed, the combination of cisplatin and <i>M. orbicularis</i> fruit extracts was more cytotoxic and induced more aggregation of A549 cells than either treatment alone. GC-MS analysis and total polyphenol and flavonoid content determination indicated that <i>M. orbicularis</i> is rich in compounds that have anti-cancerous effects. We propose <i>M. orbicularis</i> as a potential source of anti-cancerous agents to manage the progression of lung cancer and its resistance to therapy.</p><h2>Other Information</h2><p dir="ltr">Published in: Plants<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://doi.org/10.3390/plants13030442" target="_blank">https://doi.org/10.3390/plants13030442</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_7be7430e7842e9fc1456724a6b35f478 |
| identifier_str_mv | 10.3390/plants13030442 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/30415072 |
| publishDate | 2024 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Determination of Medicago orbicularis Antioxidant, Antihemolytic, and Anti-Cancerous Activities and Its Augmentation of Cisplatin-Induced Cytotoxicity in A549 Lung Cancer CellsAbdullah Shaito (20545181)Islam Omairi (22481697)Najlaa A. Al Thani (17788493)Fatiha Seglab (22468265)Esraa Ad-Darwish (22481703)Firas Kobeissy (17849094)Salam Nasreddine (214796)Agricultural, veterinary and food sciencesCrop and pasture productionBiological sciencesBiochemistry and cell biologyBiomedical and clinical sciencesCardiovascular medicine and haematologyOncology and carcinogenesisPharmacology and pharmaceutical sciencesHealth sciencesTraditional, complementary and integrative medicineMedicago orbicularislung cancerherbal medicineantioxidantshemolyticcisplatinA549 cells<p dir="ltr">The anti-lung cancer properties of the plant <i>Medicago orbicularis</i> have not been explored yet. Therefore, we identified its phytochemical composition and investigated the antioxidant, anti-hemolytic, and anti-cancerous properties of extracts of this plant in A549 human lung adenocarcinoma cells. The results show that all parts of <i>M. orbicularis</i> (stems, leaves, and fruits) exhibit remarkable hemolytic activities and modest antioxidant capacity. In addition, all extracts showed a dose-dependent anti-cancerous cytotoxic activity against A549 cells, with fruit extracts being the most potent. This cytotoxic effect could be related, at least partly, to the induction of apoptosis, where <i>M. orbicularis</i> fruit extracts reduced the ratio of anti-apoptotic BCL-2/pro-apoptotic BAX, thereby promoting cellular death. Furthermore, the use of <i>M. orbicularis</i>, in combination with a conventional chemotherapeutic agent, cisplatin, was assessed. Indeed, the combination of cisplatin and <i>M. orbicularis</i> fruit extracts was more cytotoxic and induced more aggregation of A549 cells than either treatment alone. GC-MS analysis and total polyphenol and flavonoid content determination indicated that <i>M. orbicularis</i> is rich in compounds that have anti-cancerous effects. We propose <i>M. orbicularis</i> as a potential source of anti-cancerous agents to manage the progression of lung cancer and its resistance to therapy.</p><h2>Other Information</h2><p dir="ltr">Published in: Plants<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://doi.org/10.3390/plants13030442" target="_blank">https://doi.org/10.3390/plants13030442</a></p>2024-02-02T06:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3390/plants13030442https://figshare.com/articles/journal_contribution/Determination_of_Medicago_orbicularis_Antioxidant_Antihemolytic_and_Anti-Cancerous_Activities_and_Its_Augmentation_of_Cisplatin-Induced_Cytotoxicity_in_A549_Lung_Cancer_Cells/30415072CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/304150722024-02-02T06:00:00Z |
| spellingShingle | Determination of Medicago orbicularis Antioxidant, Antihemolytic, and Anti-Cancerous Activities and Its Augmentation of Cisplatin-Induced Cytotoxicity in A549 Lung Cancer Cells Abdullah Shaito (20545181) Agricultural, veterinary and food sciences Crop and pasture production Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Cardiovascular medicine and haematology Oncology and carcinogenesis Pharmacology and pharmaceutical sciences Health sciences Traditional, complementary and integrative medicine Medicago orbicularis lung cancer herbal medicine antioxidants hemolytic cisplatin A549 cells |
| status_str | publishedVersion |
| title | Determination of Medicago orbicularis Antioxidant, Antihemolytic, and Anti-Cancerous Activities and Its Augmentation of Cisplatin-Induced Cytotoxicity in A549 Lung Cancer Cells |
| title_full | Determination of Medicago orbicularis Antioxidant, Antihemolytic, and Anti-Cancerous Activities and Its Augmentation of Cisplatin-Induced Cytotoxicity in A549 Lung Cancer Cells |
| title_fullStr | Determination of Medicago orbicularis Antioxidant, Antihemolytic, and Anti-Cancerous Activities and Its Augmentation of Cisplatin-Induced Cytotoxicity in A549 Lung Cancer Cells |
| title_full_unstemmed | Determination of Medicago orbicularis Antioxidant, Antihemolytic, and Anti-Cancerous Activities and Its Augmentation of Cisplatin-Induced Cytotoxicity in A549 Lung Cancer Cells |
| title_short | Determination of Medicago orbicularis Antioxidant, Antihemolytic, and Anti-Cancerous Activities and Its Augmentation of Cisplatin-Induced Cytotoxicity in A549 Lung Cancer Cells |
| title_sort | Determination of Medicago orbicularis Antioxidant, Antihemolytic, and Anti-Cancerous Activities and Its Augmentation of Cisplatin-Induced Cytotoxicity in A549 Lung Cancer Cells |
| topic | Agricultural, veterinary and food sciences Crop and pasture production Biological sciences Biochemistry and cell biology Biomedical and clinical sciences Cardiovascular medicine and haematology Oncology and carcinogenesis Pharmacology and pharmaceutical sciences Health sciences Traditional, complementary and integrative medicine Medicago orbicularis lung cancer herbal medicine antioxidants hemolytic cisplatin A549 cells |