Fourier Transform Infrared Imaging—A Novel Approach to Monitor Bio Molecular Changes in Subacute Mild Traumatic Brain Injury
<div><p>Traumatic brain injury (TBI) can be defined as a disorder in the function of the brain after a bump, blow, or jolt to the head, or penetrating head injury. Mild traumatic brain injury (mTBI) can cause devastating effects, such as the initiation of long-term neurodegeneration in b...
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2021
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| _version_ | 1864513517671940096 |
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| author | Fazle Rakib (5768069) |
| author2 | Khalid Al-Saad (5768084) Sebnem Garip Ustaoglu (18460611) Ehsan Ullah (2698921) Raghvendra Mall (581171) Richard Thompson (47003) Essam M. Abdelalim (5768072) Tariq Ahmed (5768081) Feride Severcan (1516903) Mohamed H. M. Ali (5768066) |
| author2_role | author author author author author author author author author |
| author_facet | Fazle Rakib (5768069) Khalid Al-Saad (5768084) Sebnem Garip Ustaoglu (18460611) Ehsan Ullah (2698921) Raghvendra Mall (581171) Richard Thompson (47003) Essam M. Abdelalim (5768072) Tariq Ahmed (5768081) Feride Severcan (1516903) Mohamed H. M. Ali (5768066) |
| author_role | author |
| dc.creator.none.fl_str_mv | Fazle Rakib (5768069) Khalid Al-Saad (5768084) Sebnem Garip Ustaoglu (18460611) Ehsan Ullah (2698921) Raghvendra Mall (581171) Richard Thompson (47003) Essam M. Abdelalim (5768072) Tariq Ahmed (5768081) Feride Severcan (1516903) Mohamed H. M. Ali (5768066) |
| dc.date.none.fl_str_mv | 2021-07-12T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.3390/brainsci11070918 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Fourier_Transform_Infrared_Imaging_A_Novel_Approach_to_Monitor_Bio_Molecular_Changes_in_Subacute_Mild_Traumatic_Brain_Injury/25713798 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biomedical and clinical sciences Neurosciences fourier transform infrared traumatic brain injury biochemical analysis and immunohistochemistry |
| dc.title.none.fl_str_mv | Fourier Transform Infrared Imaging—A Novel Approach to Monitor Bio Molecular Changes in Subacute Mild Traumatic Brain Injury |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <div><p>Traumatic brain injury (TBI) can be defined as a disorder in the function of the brain after a bump, blow, or jolt to the head, or penetrating head injury. Mild traumatic brain injury (mTBI) can cause devastating effects, such as the initiation of long-term neurodegeneration in brain tissue. In the current study, the effects of mTBI were investigated on rat brain regions; cortex (Co) and corpus callosum (CC) after 24 h (subacute trauma) by Fourier transform infrared (FTIR) imaging and immunohistochemistry (IHC). IHC studies showed the formation of amyloid-β (Aβ) plaques in the cortex brain region of mTBI rats. Moreover, staining of myelin basic protein presented the shearing of axons in CC region in the same group of animals. According to FTIR imaging results, total protein and lipid content significantly decreased in both Co and CC regions in mTBI group compared to the control. Due to this significant decrease in both lipid and protein content, remarkable consistency in lipid/protein band ratio in mTBI and control group, was observed. Significant decrease in methyl content and a significant increase in olefinic content were observed in Co and CC regions of mTBI rat brain tissues. Classification amongst distinguishable groups was performed using principal component analysis (PCA) and hierarchical clustering (HCA). This study established the prospective of FTIR imaging for assessing biochemical changes due to mTBI with high sensitivity, precision and high-resolution.</p><p> </p></div><h2>Other Information</h2> <p> Published in: Brain Sciences<br> License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/brainsci11070918" target="_blank">https://dx.doi.org/10.3390/brainsci11070918</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_7ee31d3bb8516eb59b89aed0d3046673 |
| identifier_str_mv | 10.3390/brainsci11070918 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/25713798 |
| publishDate | 2021 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Fourier Transform Infrared Imaging—A Novel Approach to Monitor Bio Molecular Changes in Subacute Mild Traumatic Brain InjuryFazle Rakib (5768069)Khalid Al-Saad (5768084)Sebnem Garip Ustaoglu (18460611)Ehsan Ullah (2698921)Raghvendra Mall (581171)Richard Thompson (47003)Essam M. Abdelalim (5768072)Tariq Ahmed (5768081)Feride Severcan (1516903)Mohamed H. M. Ali (5768066)Biomedical and clinical sciencesNeurosciencesfourier transform infraredtraumatic brain injurybiochemical analysis and immunohistochemistry<div><p>Traumatic brain injury (TBI) can be defined as a disorder in the function of the brain after a bump, blow, or jolt to the head, or penetrating head injury. Mild traumatic brain injury (mTBI) can cause devastating effects, such as the initiation of long-term neurodegeneration in brain tissue. In the current study, the effects of mTBI were investigated on rat brain regions; cortex (Co) and corpus callosum (CC) after 24 h (subacute trauma) by Fourier transform infrared (FTIR) imaging and immunohistochemistry (IHC). IHC studies showed the formation of amyloid-β (Aβ) plaques in the cortex brain region of mTBI rats. Moreover, staining of myelin basic protein presented the shearing of axons in CC region in the same group of animals. According to FTIR imaging results, total protein and lipid content significantly decreased in both Co and CC regions in mTBI group compared to the control. Due to this significant decrease in both lipid and protein content, remarkable consistency in lipid/protein band ratio in mTBI and control group, was observed. Significant decrease in methyl content and a significant increase in olefinic content were observed in Co and CC regions of mTBI rat brain tissues. Classification amongst distinguishable groups was performed using principal component analysis (PCA) and hierarchical clustering (HCA). This study established the prospective of FTIR imaging for assessing biochemical changes due to mTBI with high sensitivity, precision and high-resolution.</p><p> </p></div><h2>Other Information</h2> <p> Published in: Brain Sciences<br> License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/brainsci11070918" target="_blank">https://dx.doi.org/10.3390/brainsci11070918</a></p>2021-07-12T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3390/brainsci11070918https://figshare.com/articles/journal_contribution/Fourier_Transform_Infrared_Imaging_A_Novel_Approach_to_Monitor_Bio_Molecular_Changes_in_Subacute_Mild_Traumatic_Brain_Injury/25713798CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/257137982021-07-12T03:00:00Z |
| spellingShingle | Fourier Transform Infrared Imaging—A Novel Approach to Monitor Bio Molecular Changes in Subacute Mild Traumatic Brain Injury Fazle Rakib (5768069) Biomedical and clinical sciences Neurosciences fourier transform infrared traumatic brain injury biochemical analysis and immunohistochemistry |
| status_str | publishedVersion |
| title | Fourier Transform Infrared Imaging—A Novel Approach to Monitor Bio Molecular Changes in Subacute Mild Traumatic Brain Injury |
| title_full | Fourier Transform Infrared Imaging—A Novel Approach to Monitor Bio Molecular Changes in Subacute Mild Traumatic Brain Injury |
| title_fullStr | Fourier Transform Infrared Imaging—A Novel Approach to Monitor Bio Molecular Changes in Subacute Mild Traumatic Brain Injury |
| title_full_unstemmed | Fourier Transform Infrared Imaging—A Novel Approach to Monitor Bio Molecular Changes in Subacute Mild Traumatic Brain Injury |
| title_short | Fourier Transform Infrared Imaging—A Novel Approach to Monitor Bio Molecular Changes in Subacute Mild Traumatic Brain Injury |
| title_sort | Fourier Transform Infrared Imaging—A Novel Approach to Monitor Bio Molecular Changes in Subacute Mild Traumatic Brain Injury |
| topic | Biomedical and clinical sciences Neurosciences fourier transform infrared traumatic brain injury biochemical analysis and immunohistochemistry |