Smart fluorescence graphene quantum dot-based on-off sensor for tracing antibiotic residues in milk
<p dir="ltr">The daily use of tetracycline antibiotics in human and animal practices has led to the accumulation of drug residues in food products, potentially fostering the development of antibiotic-resistant bacteria. This study introduces the synthesis and the characterization of...
محفوظ في:
| المؤلف الرئيسي: | |
|---|---|
| مؤلفون آخرون: | , , , |
| منشور في: |
2025
|
| الموضوعات: | |
| الوسوم: |
إضافة وسم
لا توجد وسوم, كن أول من يضع وسما على هذه التسجيلة!
|
| الملخص: | <p dir="ltr">The daily use of tetracycline antibiotics in human and animal practices has led to the accumulation of drug residues in food products, potentially fostering the development of antibiotic-resistant bacteria. This study introduces the synthesis and the characterization of a fast and ultra-sensitive nanoprobe designed for label-free detection of tetracycline hydrochloride (TET) as per se and in milk samples. The nanoprobe was synthesized using graphene oxide (GO) via a simple hydrothermal procedure. Plackett-Burman (PB) design was applied to adjust process parameters over two phases: synthesis and analysis. In the synthesis rehearsal, the objective was set to maximize the quantum yield (%QY) of the graphene quantum dots (GQDs). In this itinerary, GQDs with a %QY of 12.89% were obtained using acetonitrile as a solvent at 200 ℃ for 4 h. GQDs exhibited strong fluorescence with excitation and emission wavelengths of 330 nm and 407 nm, respectively. TEM analysis confirmed the formation of uniform spherical GQDs with a particle size of 1.95 ± 0.32 nm. FT-IR revealed the existence of different moieties on the GQDs, including C–H bending, C = C stretching, and C–O bonds. The prepared fluorescent probe demonstrated high selectivity for TET over other antibiotics and metal ions. For the analysis phase, objective was set as maximum fluorescence quenching (%Q). TET detection sensitivity was maximized using contact time of only 120 s, pH 9.0, and at 55℃. A multilinear calibration curve was obtained within the range of 0.69–23.99 µM TET, with a detection limit (LOD) of 0.015 µM.</p><h2 dir="ltr">Other Information</h2><p dir="ltr">Published in: Emergent Materials<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1007/s42247-025-01230-5" target="_blank">https://dx.doi.org/10.1007/s42247-025-01230-5</a></p> |
|---|