Smart fluorescence graphene quantum dot-based on-off sensor for tracing antibiotic residues in milk
<p dir="ltr">The daily use of tetracycline antibiotics in human and animal practices has led to the accumulation of drug residues in food products, potentially fostering the development of antibiotic-resistant bacteria. This study introduces the synthesis and the characterization of...
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| مؤلفون آخرون: | , , , |
| منشور في: |
2025
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| _version_ | 1864513524540112896 |
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| author | Ahmed S. El-Shafie (14151696) |
| author2 | Sumyah Hassan (21663420) Saeed Al-Meer (7355084) Khalid Al-Saad (5768084) Marwa El-Azazy (14152794) |
| author2_role | author author author author |
| author_facet | Ahmed S. El-Shafie (14151696) Sumyah Hassan (21663420) Saeed Al-Meer (7355084) Khalid Al-Saad (5768084) Marwa El-Azazy (14152794) |
| author_role | author |
| dc.creator.none.fl_str_mv | Ahmed S. El-Shafie (14151696) Sumyah Hassan (21663420) Saeed Al-Meer (7355084) Khalid Al-Saad (5768084) Marwa El-Azazy (14152794) |
| dc.date.none.fl_str_mv | 2025-09-08T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1007/s42247-025-01230-5 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Smart_fluorescence_graphene_quantum_dot-based_on-off_sensor_for_tracing_antibiotic_residues_in_milk/31056763 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Agricultural, veterinary and food sciences Food sciences Biomedical and clinical sciences Pharmacology and pharmaceutical sciences Engineering Nanotechnology Graphene quantum dots Tetracycline hydrochloride Plackett-Burman (PB) design On-off sensor |
| dc.title.none.fl_str_mv | Smart fluorescence graphene quantum dot-based on-off sensor for tracing antibiotic residues in milk |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">The daily use of tetracycline antibiotics in human and animal practices has led to the accumulation of drug residues in food products, potentially fostering the development of antibiotic-resistant bacteria. This study introduces the synthesis and the characterization of a fast and ultra-sensitive nanoprobe designed for label-free detection of tetracycline hydrochloride (TET) as per se and in milk samples. The nanoprobe was synthesized using graphene oxide (GO) via a simple hydrothermal procedure. Plackett-Burman (PB) design was applied to adjust process parameters over two phases: synthesis and analysis. In the synthesis rehearsal, the objective was set to maximize the quantum yield (%QY) of the graphene quantum dots (GQDs). In this itinerary, GQDs with a %QY of 12.89% were obtained using acetonitrile as a solvent at 200 ℃ for 4 h. GQDs exhibited strong fluorescence with excitation and emission wavelengths of 330 nm and 407 nm, respectively. TEM analysis confirmed the formation of uniform spherical GQDs with a particle size of 1.95 ± 0.32 nm. FT-IR revealed the existence of different moieties on the GQDs, including C–H bending, C = C stretching, and C–O bonds. The prepared fluorescent probe demonstrated high selectivity for TET over other antibiotics and metal ions. For the analysis phase, objective was set as maximum fluorescence quenching (%Q). TET detection sensitivity was maximized using contact time of only 120 s, pH 9.0, and at 55℃. A multilinear calibration curve was obtained within the range of 0.69–23.99 µM TET, with a detection limit (LOD) of 0.015 µM.</p><h2 dir="ltr">Other Information</h2><p dir="ltr">Published in: Emergent Materials<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1007/s42247-025-01230-5" target="_blank">https://dx.doi.org/10.1007/s42247-025-01230-5</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_7f0dcb824b524c12a75f8473045bddef |
| identifier_str_mv | 10.1007/s42247-025-01230-5 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/31056763 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Smart fluorescence graphene quantum dot-based on-off sensor for tracing antibiotic residues in milkAhmed S. El-Shafie (14151696)Sumyah Hassan (21663420)Saeed Al-Meer (7355084)Khalid Al-Saad (5768084)Marwa El-Azazy (14152794)Agricultural, veterinary and food sciencesFood sciencesBiomedical and clinical sciencesPharmacology and pharmaceutical sciencesEngineeringNanotechnologyGraphene quantum dotsTetracycline hydrochloridePlackett-Burman (PB) designOn-off sensor<p dir="ltr">The daily use of tetracycline antibiotics in human and animal practices has led to the accumulation of drug residues in food products, potentially fostering the development of antibiotic-resistant bacteria. This study introduces the synthesis and the characterization of a fast and ultra-sensitive nanoprobe designed for label-free detection of tetracycline hydrochloride (TET) as per se and in milk samples. The nanoprobe was synthesized using graphene oxide (GO) via a simple hydrothermal procedure. Plackett-Burman (PB) design was applied to adjust process parameters over two phases: synthesis and analysis. In the synthesis rehearsal, the objective was set to maximize the quantum yield (%QY) of the graphene quantum dots (GQDs). In this itinerary, GQDs with a %QY of 12.89% were obtained using acetonitrile as a solvent at 200 ℃ for 4 h. GQDs exhibited strong fluorescence with excitation and emission wavelengths of 330 nm and 407 nm, respectively. TEM analysis confirmed the formation of uniform spherical GQDs with a particle size of 1.95 ± 0.32 nm. FT-IR revealed the existence of different moieties on the GQDs, including C–H bending, C = C stretching, and C–O bonds. The prepared fluorescent probe demonstrated high selectivity for TET over other antibiotics and metal ions. For the analysis phase, objective was set as maximum fluorescence quenching (%Q). TET detection sensitivity was maximized using contact time of only 120 s, pH 9.0, and at 55℃. A multilinear calibration curve was obtained within the range of 0.69–23.99 µM TET, with a detection limit (LOD) of 0.015 µM.</p><h2 dir="ltr">Other Information</h2><p dir="ltr">Published in: Emergent Materials<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1007/s42247-025-01230-5" target="_blank">https://dx.doi.org/10.1007/s42247-025-01230-5</a></p>2025-09-08T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1007/s42247-025-01230-5https://figshare.com/articles/journal_contribution/Smart_fluorescence_graphene_quantum_dot-based_on-off_sensor_for_tracing_antibiotic_residues_in_milk/31056763CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/310567632025-09-08T03:00:00Z |
| spellingShingle | Smart fluorescence graphene quantum dot-based on-off sensor for tracing antibiotic residues in milk Ahmed S. El-Shafie (14151696) Agricultural, veterinary and food sciences Food sciences Biomedical and clinical sciences Pharmacology and pharmaceutical sciences Engineering Nanotechnology Graphene quantum dots Tetracycline hydrochloride Plackett-Burman (PB) design On-off sensor |
| status_str | publishedVersion |
| title | Smart fluorescence graphene quantum dot-based on-off sensor for tracing antibiotic residues in milk |
| title_full | Smart fluorescence graphene quantum dot-based on-off sensor for tracing antibiotic residues in milk |
| title_fullStr | Smart fluorescence graphene quantum dot-based on-off sensor for tracing antibiotic residues in milk |
| title_full_unstemmed | Smart fluorescence graphene quantum dot-based on-off sensor for tracing antibiotic residues in milk |
| title_short | Smart fluorescence graphene quantum dot-based on-off sensor for tracing antibiotic residues in milk |
| title_sort | Smart fluorescence graphene quantum dot-based on-off sensor for tracing antibiotic residues in milk |
| topic | Agricultural, veterinary and food sciences Food sciences Biomedical and clinical sciences Pharmacology and pharmaceutical sciences Engineering Nanotechnology Graphene quantum dots Tetracycline hydrochloride Plackett-Burman (PB) design On-off sensor |