Tolfenamic Acid Derivatives: A New Class of Transcriptional Modulators with Potential Therapeutic Applications for Alzheimer’s Disease and Related Disorders

<p dir="ltr">The field of Alzheimer’s disease (AD) has witnessed recent breakthroughs in the development of disease-modifying biologics and diagnostic markers. While immunotherapeutic interventions have provided much-awaited solutions, nucleic acid-based tools represent other avenues...

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Main Author: Jaunetta Hill (18255631) (author)
Other Authors: Karim E. Shalaby (12267224) (author), Syed W. Bihaqi (19457386) (author), Bothaina H. Alansi (19457389) (author), Benjamin Barlock (13119613) (author), Keykavous Parang (281303) (author), Richard Thompson (47003) (author), Khalid Ouararhni (3145734) (author), Nasser H. Zawia (18255646) (author)
Published: 2023
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_version_ 1864513507590930432
author Jaunetta Hill (18255631)
author2 Karim E. Shalaby (12267224)
Syed W. Bihaqi (19457386)
Bothaina H. Alansi (19457389)
Benjamin Barlock (13119613)
Keykavous Parang (281303)
Richard Thompson (47003)
Khalid Ouararhni (3145734)
Nasser H. Zawia (18255646)
author2_role author
author
author
author
author
author
author
author
author_facet Jaunetta Hill (18255631)
Karim E. Shalaby (12267224)
Syed W. Bihaqi (19457386)
Bothaina H. Alansi (19457389)
Benjamin Barlock (13119613)
Keykavous Parang (281303)
Richard Thompson (47003)
Khalid Ouararhni (3145734)
Nasser H. Zawia (18255646)
author_role author
dc.creator.none.fl_str_mv Jaunetta Hill (18255631)
Karim E. Shalaby (12267224)
Syed W. Bihaqi (19457386)
Bothaina H. Alansi (19457389)
Benjamin Barlock (13119613)
Keykavous Parang (281303)
Richard Thompson (47003)
Khalid Ouararhni (3145734)
Nasser H. Zawia (18255646)
dc.date.none.fl_str_mv 2023-10-16T09:00:00Z
dc.identifier.none.fl_str_mv 10.3390/ijms242015216
dc.relation.none.fl_str_mv https://figshare.com/articles/journal_contribution/Tolfenamic_Acid_Derivatives_A_New_Class_of_Transcriptional_Modulators_with_Potential_Therapeutic_Applications_for_Alzheimer_s_Disease_and_Related_Disorders/26796232
dc.rights.none.fl_str_mv CC BY 4.0
info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv Biomedical and clinical sciences
Clinical sciences
Neurosciences
Pharmacology and pharmaceutical sciences
Alzheimer’s disease
drug design
drug development
small molecules
tolfenamic acid
SP1
dc.title.none.fl_str_mv Tolfenamic Acid Derivatives: A New Class of Transcriptional Modulators with Potential Therapeutic Applications for Alzheimer’s Disease and Related Disorders
dc.type.none.fl_str_mv Text
Journal contribution
info:eu-repo/semantics/publishedVersion
text
contribution to journal
description <p dir="ltr">The field of Alzheimer’s disease (AD) has witnessed recent breakthroughs in the development of disease-modifying biologics and diagnostic markers. While immunotherapeutic interventions have provided much-awaited solutions, nucleic acid-based tools represent other avenues of intervention; however, these approaches are costly and invasive, and they have serious side effects. Previously, we have shown in AD animal models that tolfenamic acid (TA) can lower the expression of AD-related genes and their products and subsequently reduce pathological burden and improve cognition. Using TA as a scaffold and the zinc finger domain of SP1 as a pharmacophore, we developed safer and more potent brain-penetrating analogs that interfere with sequence-specific DNA binding at transcription start sites and predominantly modulate the expression of SP1 target genes. More importantly, the proteome of treated cells displayed ~75% of the downregulated products as SP1 targets. Specific levels of SP1-driven genes and AD biomarkers such as amyloid precursor protein (APP) and Tau proteins were also decreased as part of this targeted systemic response. These small molecules, therefore, offer a viable alternative to achieving desired therapeutic outcomes by interfering with both amyloid and Tau pathways with limited off-target systemic changes.</p><h2>Other Information</h2><p dir="ltr">Published in: International Journal of Molecular Sciences<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/ijms242015216" target="_blank">https://dx.doi.org/10.3390/ijms242015216</a></p>
eu_rights_str_mv openAccess
id Manara2_8323a9a39ed9146efa297cf68b43728e
identifier_str_mv 10.3390/ijms242015216
network_acronym_str Manara2
network_name_str Manara2
oai_identifier_str oai:figshare.com:article/26796232
publishDate 2023
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rights_invalid_str_mv CC BY 4.0
spelling Tolfenamic Acid Derivatives: A New Class of Transcriptional Modulators with Potential Therapeutic Applications for Alzheimer’s Disease and Related DisordersJaunetta Hill (18255631)Karim E. Shalaby (12267224)Syed W. Bihaqi (19457386)Bothaina H. Alansi (19457389)Benjamin Barlock (13119613)Keykavous Parang (281303)Richard Thompson (47003)Khalid Ouararhni (3145734)Nasser H. Zawia (18255646)Biomedical and clinical sciencesClinical sciencesNeurosciencesPharmacology and pharmaceutical sciencesAlzheimer’s diseasedrug designdrug developmentsmall moleculestolfenamic acidSP1<p dir="ltr">The field of Alzheimer’s disease (AD) has witnessed recent breakthroughs in the development of disease-modifying biologics and diagnostic markers. While immunotherapeutic interventions have provided much-awaited solutions, nucleic acid-based tools represent other avenues of intervention; however, these approaches are costly and invasive, and they have serious side effects. Previously, we have shown in AD animal models that tolfenamic acid (TA) can lower the expression of AD-related genes and their products and subsequently reduce pathological burden and improve cognition. Using TA as a scaffold and the zinc finger domain of SP1 as a pharmacophore, we developed safer and more potent brain-penetrating analogs that interfere with sequence-specific DNA binding at transcription start sites and predominantly modulate the expression of SP1 target genes. More importantly, the proteome of treated cells displayed ~75% of the downregulated products as SP1 targets. Specific levels of SP1-driven genes and AD biomarkers such as amyloid precursor protein (APP) and Tau proteins were also decreased as part of this targeted systemic response. These small molecules, therefore, offer a viable alternative to achieving desired therapeutic outcomes by interfering with both amyloid and Tau pathways with limited off-target systemic changes.</p><h2>Other Information</h2><p dir="ltr">Published in: International Journal of Molecular Sciences<br>License: <a href="https://creativecommons.org/licenses/by/4.0/" target="_blank">https://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.3390/ijms242015216" target="_blank">https://dx.doi.org/10.3390/ijms242015216</a></p>2023-10-16T09:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.3390/ijms242015216https://figshare.com/articles/journal_contribution/Tolfenamic_Acid_Derivatives_A_New_Class_of_Transcriptional_Modulators_with_Potential_Therapeutic_Applications_for_Alzheimer_s_Disease_and_Related_Disorders/26796232CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/267962322023-10-16T09:00:00Z
spellingShingle Tolfenamic Acid Derivatives: A New Class of Transcriptional Modulators with Potential Therapeutic Applications for Alzheimer’s Disease and Related Disorders
Jaunetta Hill (18255631)
Biomedical and clinical sciences
Clinical sciences
Neurosciences
Pharmacology and pharmaceutical sciences
Alzheimer’s disease
drug design
drug development
small molecules
tolfenamic acid
SP1
status_str publishedVersion
title Tolfenamic Acid Derivatives: A New Class of Transcriptional Modulators with Potential Therapeutic Applications for Alzheimer’s Disease and Related Disorders
title_full Tolfenamic Acid Derivatives: A New Class of Transcriptional Modulators with Potential Therapeutic Applications for Alzheimer’s Disease and Related Disorders
title_fullStr Tolfenamic Acid Derivatives: A New Class of Transcriptional Modulators with Potential Therapeutic Applications for Alzheimer’s Disease and Related Disorders
title_full_unstemmed Tolfenamic Acid Derivatives: A New Class of Transcriptional Modulators with Potential Therapeutic Applications for Alzheimer’s Disease and Related Disorders
title_short Tolfenamic Acid Derivatives: A New Class of Transcriptional Modulators with Potential Therapeutic Applications for Alzheimer’s Disease and Related Disorders
title_sort Tolfenamic Acid Derivatives: A New Class of Transcriptional Modulators with Potential Therapeutic Applications for Alzheimer’s Disease and Related Disorders
topic Biomedical and clinical sciences
Clinical sciences
Neurosciences
Pharmacology and pharmaceutical sciences
Alzheimer’s disease
drug design
drug development
small molecules
tolfenamic acid
SP1