Antioxidant Activity Mediates Pirfenidone Antifibrotic Effects in Human Pulmonary Vascular Smooth Muscle Cells Exposed to Sera of Idiopathic Pulmonary Fibrosis Patients
<p>Idiopathic pulmonary fibrosis (IPF) is a chronic lung disease characterized by an exacerbated fibrotic response. Although molecular and cellular determinants involved in the onset and progression of this devastating disease are largely unknown, an aberrant remodeling of the pulmonary vascul...
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| مؤلفون آخرون: | , , , , , , , , |
| منشور في: |
2018
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| _version_ | 1864513563226275840 |
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| author | Alessandro Giuseppe Fois (2901365) |
| author2 | Anna Maria Posadino (299594) Roberta Giordo (299595) Annalisa Cossu (299593) Abdelali Agouni (14152272) Nasser Moustafa Rizk (14603368) Pietro Pirina (411999) Ciriaco Carru (276952) Angelo Zinellu (276951) Gianfranco Pintus (14603369) |
| author2_role | author author author author author author author author author |
| author_facet | Alessandro Giuseppe Fois (2901365) Anna Maria Posadino (299594) Roberta Giordo (299595) Annalisa Cossu (299593) Abdelali Agouni (14152272) Nasser Moustafa Rizk (14603368) Pietro Pirina (411999) Ciriaco Carru (276952) Angelo Zinellu (276951) Gianfranco Pintus (14603369) |
| author_role | author |
| dc.creator.none.fl_str_mv | Alessandro Giuseppe Fois (2901365) Anna Maria Posadino (299594) Roberta Giordo (299595) Annalisa Cossu (299593) Abdelali Agouni (14152272) Nasser Moustafa Rizk (14603368) Pietro Pirina (411999) Ciriaco Carru (276952) Angelo Zinellu (276951) Gianfranco Pintus (14603369) |
| dc.date.none.fl_str_mv | 2018-10-21T03:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1155/2018/2639081 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Antioxidant_Activity_Mediates_Pirfenidone_Antifibrotic_Effects_in_Human_Pulmonary_Vascular_Smooth_Muscle_Cells_Exposed_to_Sera_of_Idiopathic_Pulmonary_Fibrosis_Patients/22082873 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Biochemistry and cell biology Cell Biology Aging General Medicine Biochemistry |
| dc.title.none.fl_str_mv | Antioxidant Activity Mediates Pirfenidone Antifibrotic Effects in Human Pulmonary Vascular Smooth Muscle Cells Exposed to Sera of Idiopathic Pulmonary Fibrosis Patients |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p>Idiopathic pulmonary fibrosis (IPF) is a chronic lung disease characterized by an exacerbated fibrotic response. Although molecular and cellular determinants involved in the onset and progression of this devastating disease are largely unknown, an aberrant remodeling of the pulmonary vasculature appears to have implications in IPF pathogenesis. Here, we demonstrated for the first time that an increase of reactive oxygen species (ROS) generation induced by sera from IPF patients drives both collagen type I deposition and proliferation of primary human pulmonary artery smooth muscle cells (HPASMCs). IPF sera-induced cellular effects were significantly blunted in cells exposed to the NADPH oxidase inhibitor diphenyleneiodonium (DPI) proving the causative role of ROS and suggesting their potential cellular source. Contrary to IPF naive patients, sera from Pirfenidone-treated IPF patients failed to significantly induce both ROS generation and collagen synthesis in HPASMCs, mechanistically implicating antioxidant properties as the basis for the in vivo effect of this drug. </p> <h2>Other information</h2> <p>Published in: Oxidative Medicine and Cellular Longevity<br> License: <a href="http://creativecommons.org/licenses/by/4.0" target="_blank">http://creativecommons.org/licenses/by/4.0</a><br> See article on publisher's website: <a href="http://dx.doi.org/10.1155/2018/2639081" target="_blank">http://dx.doi.org/10.1155/2018/2639081</a> </p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_859a86433bcff831d3a19673b3c67340 |
| identifier_str_mv | 10.1155/2018/2639081 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/22082873 |
| publishDate | 2018 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Antioxidant Activity Mediates Pirfenidone Antifibrotic Effects in Human Pulmonary Vascular Smooth Muscle Cells Exposed to Sera of Idiopathic Pulmonary Fibrosis PatientsAlessandro Giuseppe Fois (2901365)Anna Maria Posadino (299594)Roberta Giordo (299595)Annalisa Cossu (299593)Abdelali Agouni (14152272)Nasser Moustafa Rizk (14603368)Pietro Pirina (411999)Ciriaco Carru (276952)Angelo Zinellu (276951)Gianfranco Pintus (14603369)Biological sciencesBiochemistry and cell biologyCell BiologyAgingGeneral MedicineBiochemistry<p>Idiopathic pulmonary fibrosis (IPF) is a chronic lung disease characterized by an exacerbated fibrotic response. Although molecular and cellular determinants involved in the onset and progression of this devastating disease are largely unknown, an aberrant remodeling of the pulmonary vasculature appears to have implications in IPF pathogenesis. Here, we demonstrated for the first time that an increase of reactive oxygen species (ROS) generation induced by sera from IPF patients drives both collagen type I deposition and proliferation of primary human pulmonary artery smooth muscle cells (HPASMCs). IPF sera-induced cellular effects were significantly blunted in cells exposed to the NADPH oxidase inhibitor diphenyleneiodonium (DPI) proving the causative role of ROS and suggesting their potential cellular source. Contrary to IPF naive patients, sera from Pirfenidone-treated IPF patients failed to significantly induce both ROS generation and collagen synthesis in HPASMCs, mechanistically implicating antioxidant properties as the basis for the in vivo effect of this drug. </p> <h2>Other information</h2> <p>Published in: Oxidative Medicine and Cellular Longevity<br> License: <a href="http://creativecommons.org/licenses/by/4.0" target="_blank">http://creativecommons.org/licenses/by/4.0</a><br> See article on publisher's website: <a href="http://dx.doi.org/10.1155/2018/2639081" target="_blank">http://dx.doi.org/10.1155/2018/2639081</a> </p>2018-10-21T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1155/2018/2639081https://figshare.com/articles/journal_contribution/Antioxidant_Activity_Mediates_Pirfenidone_Antifibrotic_Effects_in_Human_Pulmonary_Vascular_Smooth_Muscle_Cells_Exposed_to_Sera_of_Idiopathic_Pulmonary_Fibrosis_Patients/22082873CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/220828732018-10-21T03:00:00Z |
| spellingShingle | Antioxidant Activity Mediates Pirfenidone Antifibrotic Effects in Human Pulmonary Vascular Smooth Muscle Cells Exposed to Sera of Idiopathic Pulmonary Fibrosis Patients Alessandro Giuseppe Fois (2901365) Biological sciences Biochemistry and cell biology Cell Biology Aging General Medicine Biochemistry |
| status_str | publishedVersion |
| title | Antioxidant Activity Mediates Pirfenidone Antifibrotic Effects in Human Pulmonary Vascular Smooth Muscle Cells Exposed to Sera of Idiopathic Pulmonary Fibrosis Patients |
| title_full | Antioxidant Activity Mediates Pirfenidone Antifibrotic Effects in Human Pulmonary Vascular Smooth Muscle Cells Exposed to Sera of Idiopathic Pulmonary Fibrosis Patients |
| title_fullStr | Antioxidant Activity Mediates Pirfenidone Antifibrotic Effects in Human Pulmonary Vascular Smooth Muscle Cells Exposed to Sera of Idiopathic Pulmonary Fibrosis Patients |
| title_full_unstemmed | Antioxidant Activity Mediates Pirfenidone Antifibrotic Effects in Human Pulmonary Vascular Smooth Muscle Cells Exposed to Sera of Idiopathic Pulmonary Fibrosis Patients |
| title_short | Antioxidant Activity Mediates Pirfenidone Antifibrotic Effects in Human Pulmonary Vascular Smooth Muscle Cells Exposed to Sera of Idiopathic Pulmonary Fibrosis Patients |
| title_sort | Antioxidant Activity Mediates Pirfenidone Antifibrotic Effects in Human Pulmonary Vascular Smooth Muscle Cells Exposed to Sera of Idiopathic Pulmonary Fibrosis Patients |
| topic | Biological sciences Biochemistry and cell biology Cell Biology Aging General Medicine Biochemistry |