A PROTOCOL FOR THE DETECTION OF GENETIC MARKERS IN SALIVA BY POLYMERASE CHAIN REACTION WITHOUT A NUCLEIC ACID PURIFICATION STEP: EXAMPLES OF SARS-COV-2 AND GAPDH MARKERS

A PROTOCOL FOR THE DETECTION OF GENETIC MARKERS IN SALIVA BY POLYMERASE CHAIN REACTION WITHOUT A NUCLEIC ACID PURIFICATION STEP: EXAMPLES OF SARS-COV-2 AND GAPDH MARKERS

<h3>Introduction</h3><p dir="ltr">Polymerase chain reaction (PCR)-based diagnostic tests use purifi ed nucleic acids (NAs) from clinical samples. The NAs purifi cation step adds time, cost, and aff ects the quality of testing. The objective of this study was to develop a...

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Main Author:	Surya Kannan (18456708) (author)
Other Authors:	Johan Ericsson (49714) (author), Nazariy Souchelnytskyi (80319) (author), Serhiy Souchelnytskyi (167532) (author)
Published:	2021
Subjects:	Biological sciences Biochemistry and cell biology Genetics saliva sample collection direct detection RT-PCR LAMP
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