International external quality assurance of JAK2 V617F quantification

<p dir="ltr">External quality assurance (EQA) programs are vital to ensure high quality and standardized results in molecular diagnostics. It is important that EQA for quantitative analysis takes into account the variation in methodology. Results cannot be expected to be more accurat...

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Main Author:	Julia Asp (16817103) (author)
Other Authors:	Vibe Skov (92924) (author), Beatriz Bellosillo (223997) (author), Thomas Kristensen (3481958) (author), Eric Lippert (199849) (author), Frank Dicker (19684903) (author), Jiri Schwarz (19684906) (author), Marzena Wojtaszewska (6472307) (author), Lars Palmqvist (78127) (author), Susanna Akiki (19684909) (author), Anni Aggerholm (5755373) (author), Morten Tolstrup Andersen (19684912) (author), François Girodon (818265) (author), Lasse Kjær (180444) (author), Elisabeth Oppliger Leibundgut (13845645) (author), Alessandro Pancrazzi (13566727) (author), Marta Vorland (19684915) (author), Hajnalka Andrikovics (120123) (author), Robert Kralovics (180148) (author), Bruno Cassinat (367659) (author), Margarida Coucelo (4318057) (author), Aleksandar Eftimov (19684918) (author), Karl Haslam (19684921) (author), Rajko Kusec (19684924) (author), Dorota Link-Lenczowska (19684927) (author), Laurence Lodé (19684930) (author), Karolina Matiakowska (19684933) (author), Dina Naguib (19684936) (author), Filippo Navaglia (287050) (author), Guy Wayne Novotny (3857668) (author), Melanie J Percy (19684939) (author), Andrey Sudarikov (15006459) (author), Sylvie Hermouet (8895461) (author), Niels Pallisgaard (69307) (author)
Published:	2018
Subjects:	Biological sciences Genetics Chemical sciences Medicinal and biomolecular chemistry JAK2 V617F External quality assurance Myeloproliferative neoplasms Quantitative PCR
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author	Julia Asp (16817103)
author2	Vibe Skov (92924) Beatriz Bellosillo (223997) Thomas Kristensen (3481958) Eric Lippert (199849) Frank Dicker (19684903) Jiri Schwarz (19684906) Marzena Wojtaszewska (6472307) Lars Palmqvist (78127) Susanna Akiki (19684909) Anni Aggerholm (5755373) Morten Tolstrup Andersen (19684912) François Girodon (818265) Lasse Kjær (180444) Elisabeth Oppliger Leibundgut (13845645) Alessandro Pancrazzi (13566727) Marta Vorland (19684915) Hajnalka Andrikovics (120123) Robert Kralovics (180148) Bruno Cassinat (367659) Margarida Coucelo (4318057) Aleksandar Eftimov (19684918) Karl Haslam (19684921) Rajko Kusec (19684924) Dorota Link-Lenczowska (19684927) Laurence Lodé (19684930) Karolina Matiakowska (19684933) Dina Naguib (19684936) Filippo Navaglia (287050) Guy Wayne Novotny (3857668) Melanie J Percy (19684939) Andrey Sudarikov (15006459) Sylvie Hermouet (8895461) Niels Pallisgaard (69307)
author2_role	author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author
author_facet	Julia Asp (16817103) Vibe Skov (92924) Beatriz Bellosillo (223997) Thomas Kristensen (3481958) Eric Lippert (199849) Frank Dicker (19684903) Jiri Schwarz (19684906) Marzena Wojtaszewska (6472307) Lars Palmqvist (78127) Susanna Akiki (19684909) Anni Aggerholm (5755373) Morten Tolstrup Andersen (19684912) François Girodon (818265) Lasse Kjær (180444) Elisabeth Oppliger Leibundgut (13845645) Alessandro Pancrazzi (13566727) Marta Vorland (19684915) Hajnalka Andrikovics (120123) Robert Kralovics (180148) Bruno Cassinat (367659) Margarida Coucelo (4318057) Aleksandar Eftimov (19684918) Karl Haslam (19684921) Rajko Kusec (19684924) Dorota Link-Lenczowska (19684927) Laurence Lodé (19684930) Karolina Matiakowska (19684933) Dina Naguib (19684936) Filippo Navaglia (287050) Guy Wayne Novotny (3857668) Melanie J Percy (19684939) Andrey Sudarikov (15006459) Sylvie Hermouet (8895461) Niels Pallisgaard (69307)
author_role	author
dc.creator.none.fl_str_mv	Julia Asp (16817103) Vibe Skov (92924) Beatriz Bellosillo (223997) Thomas Kristensen (3481958) Eric Lippert (199849) Frank Dicker (19684903) Jiri Schwarz (19684906) Marzena Wojtaszewska (6472307) Lars Palmqvist (78127) Susanna Akiki (19684909) Anni Aggerholm (5755373) Morten Tolstrup Andersen (19684912) François Girodon (818265) Lasse Kjær (180444) Elisabeth Oppliger Leibundgut (13845645) Alessandro Pancrazzi (13566727) Marta Vorland (19684915) Hajnalka Andrikovics (120123) Robert Kralovics (180148) Bruno Cassinat (367659) Margarida Coucelo (4318057) Aleksandar Eftimov (19684918) Karl Haslam (19684921) Rajko Kusec (19684924) Dorota Link-Lenczowska (19684927) Laurence Lodé (19684930) Karolina Matiakowska (19684933) Dina Naguib (19684936) Filippo Navaglia (287050) Guy Wayne Novotny (3857668) Melanie J Percy (19684939) Andrey Sudarikov (15006459) Sylvie Hermouet (8895461) Niels Pallisgaard (69307)
dc.date.none.fl_str_mv	2018-12-08T03:00:00Z
dc.identifier.none.fl_str_mv	10.1007/s00277-018-3570-8
dc.relation.none.fl_str_mv	https://figshare.com/articles/journal_contribution/International_external_quality_assurance_of_JAK2_V617F_quantification/27043255
dc.rights.none.fl_str_mv	CC BY 4.0 info:eu-repo/semantics/openAccess
dc.subject.none.fl_str_mv	Biological sciences Genetics Chemical sciences Medicinal and biomolecular chemistry JAK2 V617F External quality assurance Myeloproliferative neoplasms Quantitative PCR
dc.title.none.fl_str_mv	International external quality assurance of JAK2 V617F quantification
dc.type.none.fl_str_mv	Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal
description	<p dir="ltr">External quality assurance (EQA) programs are vital to ensure high quality and standardized results in molecular diagnostics. It is important that EQA for quantitative analysis takes into account the variation in methodology. Results cannot be expected to be more accurate than limits of the technology used, and it is essential to recognize factors causing substantial outlier results. The present study aimed to identify parameters of specific importance for <i>JAK2</i> V617F quantification by quantitative PCR, using different starting materials, assays, and technical platforms. Sixteen samples were issued to participating laboratories in two EQA rounds. In the first round, 19 laboratories from 11 European countries analyzing <i>JAK2</i> V617F as part of their routine diagnostics returned results from in-house assays. In the second round, 25 laboratories from 17 countries participated. Despite variations in starting material, assay set-up and instrumentation the laboratories were generally well aligned in the EQA program. However, EQA based on a single technology appears to be a valuable tool to achieve standardization of the quantification of <i>JAK2</i> V617F allelic burden.</p><h2>Other Information</h2><p dir="ltr">Published in: Annals of Hematology<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1007/s00277-018-3570-8" target="_blank">https://dx.doi.org/10.1007/s00277-018-3570-8</a></p>
eu_rights_str_mv	openAccess
id	Manara2_8a5acfcddb0a4fd7f6cee00a138f0700
identifier_str_mv	10.1007/s00277-018-3570-8
network_acronym_str	Manara2
network_name_str	Manara2
oai_identifier_str	oai:figshare.com:article/27043255
publishDate	2018
repository.mail.fl_str_mv
repository.name.fl_str_mv
repository_id_str
rights_invalid_str_mv	CC BY 4.0
spelling	International external quality assurance of JAK2 V617F quantificationJulia Asp (16817103)Vibe Skov (92924)Beatriz Bellosillo (223997)Thomas Kristensen (3481958)Eric Lippert (199849)Frank Dicker (19684903)Jiri Schwarz (19684906)Marzena Wojtaszewska (6472307)Lars Palmqvist (78127)Susanna Akiki (19684909)Anni Aggerholm (5755373)Morten Tolstrup Andersen (19684912)François Girodon (818265)Lasse Kjær (180444)Elisabeth Oppliger Leibundgut (13845645)Alessandro Pancrazzi (13566727)Marta Vorland (19684915)Hajnalka Andrikovics (120123)Robert Kralovics (180148)Bruno Cassinat (367659)Margarida Coucelo (4318057)Aleksandar Eftimov (19684918)Karl Haslam (19684921)Rajko Kusec (19684924)Dorota Link-Lenczowska (19684927)Laurence Lodé (19684930)Karolina Matiakowska (19684933)Dina Naguib (19684936)Filippo Navaglia (287050)Guy Wayne Novotny (3857668)Melanie J Percy (19684939)Andrey Sudarikov (15006459)Sylvie Hermouet (8895461)Niels Pallisgaard (69307)Biological sciencesGeneticsChemical sciencesMedicinal and biomolecular chemistryJAK2 V617FExternal quality assuranceMyeloproliferative neoplasmsQuantitative PCR<p dir="ltr">External quality assurance (EQA) programs are vital to ensure high quality and standardized results in molecular diagnostics. It is important that EQA for quantitative analysis takes into account the variation in methodology. Results cannot be expected to be more accurate than limits of the technology used, and it is essential to recognize factors causing substantial outlier results. The present study aimed to identify parameters of specific importance for <i>JAK2</i> V617F quantification by quantitative PCR, using different starting materials, assays, and technical platforms. Sixteen samples were issued to participating laboratories in two EQA rounds. In the first round, 19 laboratories from 11 European countries analyzing <i>JAK2</i> V617F as part of their routine diagnostics returned results from in-house assays. In the second round, 25 laboratories from 17 countries participated. Despite variations in starting material, assay set-up and instrumentation the laboratories were generally well aligned in the EQA program. However, EQA based on a single technology appears to be a valuable tool to achieve standardization of the quantification of <i>JAK2</i> V617F allelic burden.</p><h2>Other Information</h2><p dir="ltr">Published in: Annals of Hematology<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1007/s00277-018-3570-8" target="_blank">https://dx.doi.org/10.1007/s00277-018-3570-8</a></p>2018-12-08T03:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1007/s00277-018-3570-8https://figshare.com/articles/journal_contribution/International_external_quality_assurance_of_JAK2_V617F_quantification/27043255CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/270432552018-12-08T03:00:00Z
spellingShingle	International external quality assurance of JAK2 V617F quantification Julia Asp (16817103) Biological sciences Genetics Chemical sciences Medicinal and biomolecular chemistry JAK2 V617F External quality assurance Myeloproliferative neoplasms Quantitative PCR
status_str	publishedVersion
title	International external quality assurance of JAK2 V617F quantification
title_full	International external quality assurance of JAK2 V617F quantification
title_fullStr	International external quality assurance of JAK2 V617F quantification
title_full_unstemmed	International external quality assurance of JAK2 V617F quantification
title_short	International external quality assurance of JAK2 V617F quantification
title_sort	International external quality assurance of JAK2 V617F quantification
topic	Biological sciences Genetics Chemical sciences Medicinal and biomolecular chemistry JAK2 V617F External quality assurance Myeloproliferative neoplasms Quantitative PCR

International external quality assurance of JAK2 V617F quantification

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