Water-pipe smoking promotes epithelial–mesenchymal transition and invasion of human breast cancer cells via ERK1/ERK2 pathways
<p>With the increasing popularity of water-pipe smoking (WPS), it is critical to comprehend how WPS may affect women’s health. The main goal of this study is to identify the potential outcome of WPS on human breast cancer progression. Two breast cancer cell lines, MCF7 and BT20, were used in t...
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2018
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| _version_ | 1864513566598496256 |
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| author | Khaled W. Sadek (14153196) |
| author2 | Mahmoud Y. Haik (14153199) Anas A. Ashour (14153202) Tahira Baloch (6197423) Tahar Aboulkassim (3190059) Amber Yasmeen (5472377) Semir Vranic (3353012) Asad Zeidan (8879705) Ala-Eddin Al Moustafa (14153205) |
| author2_role | author author author author author author author author |
| author_facet | Khaled W. Sadek (14153196) Mahmoud Y. Haik (14153199) Anas A. Ashour (14153202) Tahira Baloch (6197423) Tahar Aboulkassim (3190059) Amber Yasmeen (5472377) Semir Vranic (3353012) Asad Zeidan (8879705) Ala-Eddin Al Moustafa (14153205) |
| author_role | author |
| dc.creator.none.fl_str_mv | Khaled W. Sadek (14153196) Mahmoud Y. Haik (14153199) Anas A. Ashour (14153202) Tahira Baloch (6197423) Tahar Aboulkassim (3190059) Amber Yasmeen (5472377) Semir Vranic (3353012) Asad Zeidan (8879705) Ala-Eddin Al Moustafa (14153205) |
| dc.date.none.fl_str_mv | 2018-11-13T00:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1186/s12935-018-0678-9 |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Water-pipe_smoking_promotes_epithelial_mesenchymal_transition_and_invasion_of_human_breast_cancer_cells_via_ERK1_ERK2_pathways/21598398 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Genetics Biomedical and clinical sciences Oncology and carcinogenesis Water-pipe smoking Breast cancer EMT Cell invasion Cell adhesion Erk1/Erk2 pathways |
| dc.title.none.fl_str_mv | Water-pipe smoking promotes epithelial–mesenchymal transition and invasion of human breast cancer cells via ERK1/ERK2 pathways |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p>With the increasing popularity of water-pipe smoking (WPS), it is critical to comprehend how WPS may affect women’s health. The main goal of this study is to identify the potential outcome of WPS on human breast cancer progression. Two breast cancer cell lines, MCF7 and BT20, were used in this investigation. We explored the outcome of WPS on cell morphology and cell invasion using inverted microscope and Biocoat Matrigel invasion chambers. On the other hand, Western blot was employed to study the expression patterns of key control genes of cell adhesion and invasion. Our data reveal that WPS induces epithelial–mesenchymal transition (EMT) of MCF7 and BT20 breast cancer cell lines; thus, WPS enhances cell invasion ability of both cell lines in comparison with their matched controls. More significantly, WPS provokes a down- and up-regulation of E-cadherin and focal adhesion kinase (FAK), respectively, which are important key regulators of cancer progression genes. Finally, our data point out that WPS incites the activation of Erk1/Erk2, which could be behind the stimulation of EMT and invasion as well as the deregulation of E-cadherin and FAK expression. Our data show, for the first time, that WPS initiates EMT and stimulates cell invasion of breast cancer cells, which could incite metastatic development in breast cancer patients. Thus, we believe that further studies, both in vitro and in vivo, are required to elucidate the pathogenic outcome of WPS on cancer progression of several human carcinomas including breast.</p><h2>Other Information</h2> <p> Published in: Cancer Cell International<br> License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="http://dx.doi.org/10.1186/s12935-018-0678-9" target="_blank">http://dx.doi.org/10.1186/s12935-018-0678-9</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_8ad91c30187be640e5bd80cd2d82965a |
| identifier_str_mv | 10.1186/s12935-018-0678-9 |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/21598398 |
| publishDate | 2018 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Water-pipe smoking promotes epithelial–mesenchymal transition and invasion of human breast cancer cells via ERK1/ERK2 pathwaysKhaled W. Sadek (14153196)Mahmoud Y. Haik (14153199)Anas A. Ashour (14153202)Tahira Baloch (6197423)Tahar Aboulkassim (3190059)Amber Yasmeen (5472377)Semir Vranic (3353012)Asad Zeidan (8879705)Ala-Eddin Al Moustafa (14153205)Biological sciencesGeneticsBiomedical and clinical sciencesOncology and carcinogenesisWater-pipe smokingBreast cancerEMTCell invasion Cell adhesionErk1/Erk2 pathways<p>With the increasing popularity of water-pipe smoking (WPS), it is critical to comprehend how WPS may affect women’s health. The main goal of this study is to identify the potential outcome of WPS on human breast cancer progression. Two breast cancer cell lines, MCF7 and BT20, were used in this investigation. We explored the outcome of WPS on cell morphology and cell invasion using inverted microscope and Biocoat Matrigel invasion chambers. On the other hand, Western blot was employed to study the expression patterns of key control genes of cell adhesion and invasion. Our data reveal that WPS induces epithelial–mesenchymal transition (EMT) of MCF7 and BT20 breast cancer cell lines; thus, WPS enhances cell invasion ability of both cell lines in comparison with their matched controls. More significantly, WPS provokes a down- and up-regulation of E-cadherin and focal adhesion kinase (FAK), respectively, which are important key regulators of cancer progression genes. Finally, our data point out that WPS incites the activation of Erk1/Erk2, which could be behind the stimulation of EMT and invasion as well as the deregulation of E-cadherin and FAK expression. Our data show, for the first time, that WPS initiates EMT and stimulates cell invasion of breast cancer cells, which could incite metastatic development in breast cancer patients. Thus, we believe that further studies, both in vitro and in vivo, are required to elucidate the pathogenic outcome of WPS on cancer progression of several human carcinomas including breast.</p><h2>Other Information</h2> <p> Published in: Cancer Cell International<br> License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="http://dx.doi.org/10.1186/s12935-018-0678-9" target="_blank">http://dx.doi.org/10.1186/s12935-018-0678-9</a></p>2018-11-13T00:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1186/s12935-018-0678-9https://figshare.com/articles/journal_contribution/Water-pipe_smoking_promotes_epithelial_mesenchymal_transition_and_invasion_of_human_breast_cancer_cells_via_ERK1_ERK2_pathways/21598398CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/215983982018-11-13T00:00:00Z |
| spellingShingle | Water-pipe smoking promotes epithelial–mesenchymal transition and invasion of human breast cancer cells via ERK1/ERK2 pathways Khaled W. Sadek (14153196) Biological sciences Genetics Biomedical and clinical sciences Oncology and carcinogenesis Water-pipe smoking Breast cancer EMT Cell invasion Cell adhesion Erk1/Erk2 pathways |
| status_str | publishedVersion |
| title | Water-pipe smoking promotes epithelial–mesenchymal transition and invasion of human breast cancer cells via ERK1/ERK2 pathways |
| title_full | Water-pipe smoking promotes epithelial–mesenchymal transition and invasion of human breast cancer cells via ERK1/ERK2 pathways |
| title_fullStr | Water-pipe smoking promotes epithelial–mesenchymal transition and invasion of human breast cancer cells via ERK1/ERK2 pathways |
| title_full_unstemmed | Water-pipe smoking promotes epithelial–mesenchymal transition and invasion of human breast cancer cells via ERK1/ERK2 pathways |
| title_short | Water-pipe smoking promotes epithelial–mesenchymal transition and invasion of human breast cancer cells via ERK1/ERK2 pathways |
| title_sort | Water-pipe smoking promotes epithelial–mesenchymal transition and invasion of human breast cancer cells via ERK1/ERK2 pathways |
| topic | Biological sciences Genetics Biomedical and clinical sciences Oncology and carcinogenesis Water-pipe smoking Breast cancer EMT Cell invasion Cell adhesion Erk1/Erk2 pathways |