Aberrant DNA methylation of <i>PTPRG</i> as one possible mechanism of its under‐expression in CML patients in the State of Qatar
<h3>Background</h3><p dir="ltr">Several studies showed that aberrant DNA methylation is involved in leukemia and cancer pathogenesis. Protein tyrosine phosphatase receptor gamma (PTPRG) expression is a natural inhibitory mechanism that is downregulated in chronic myeloid...
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2020
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| Summary: | <h3>Background</h3><p dir="ltr">Several studies showed that aberrant DNA methylation is involved in leukemia and cancer pathogenesis. Protein tyrosine phosphatase receptor gamma (PTPRG) expression is a natural inhibitory mechanism that is downregulated in chronic myeloid leukemia (CML) disease. The mechanism behind its downregulation has not been fully elucidated yet.</p><h3>Aim</h3><p dir="ltr">This study aimed to investigate the CpG methylation status at the <i>PTPRG</i> locus in CML patients.</p><h3>Methods</h3><p dir="ltr">Peripheral blood samples from CML patients at time of diagnosis [no tyrosine kinase inhibitors (TKIs)] (<i>n</i> = 13), failure to (TKIs) treatment (<i>n</i> = 13) and healthy controls (<i>n</i> = 6) were collected. DNA was extracted and treated with bisulfite treatment, followed by PCR, sequencing of 25 CpG sites in the promoter region and 26 CpG sites in intron-1 region of <i>PTPRG</i>. The bisulfite sequencing technique was employed as a high-resolution method.</p><h3>Results</h3><p dir="ltr">CML groups (new diagnosed and failed treatment) showed significantly higher methylation levels in the promoter and intron-1 regions of <i>PTPRG</i> compared to the healthy group. There were also significant differences in methylation levels of CpG sites in the promoter and intron-1 regions amongst the groups.</p><h3>Conclusion</h3><p dir="ltr">Aberrant methylation of <i>PTPRG</i> is potentially one of the possible mechanisms of <i>PTPRG</i> downregulation detected in CML.</p><h2>Other Information</h2><p dir="ltr">Published in: Molecular Genetics & Genomic Medicine<br>License: <a href="http://creativecommons.org/licenses/by/4.0/" target="_blank">http://creativecommons.org/licenses/by/4.0/</a><br>See article on publisher's website: <a href="http://dx.doi.org/10.1002/mgg3.1319" target="_blank">http://dx.doi.org/10.1002/mgg3.1319</a></p><p dir="ltr">Additional institutions affiliated with: Qatar University, Qatar University Health - QU, College of Health Sciences - QU HEALTH, Biomedical Research Center - QU HEALTH, Qatar Medical Genetic Center - Hamad General Hospital.</p> |
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