Gene expression analysis reveals genes related to heavy metals and produced water exposure in Synechococcus elongatus
<p dir="ltr">Produced water (PW), a major by-product of the petrochemical industry, contains a complex mixture of contaminants that limit its reuse and pose environmental risks if discharged untreated. Numerous treatment technologies have been developed to remediate this water, with...
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2025
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| _version_ | 1864513524504461312 |
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| author | Alaa Hassanien (19420603) |
| author2 | Nisar Ahmed (584617) Borbala Misfud (22997953) Hareb M. Al-Jabri (19420609) Sara Al-Marri (17075137) Tasneem Dalgamouni (19420606) Maryam Al-Merekhi (22997956) Kira Schipper (17316883) Imen Saadaoui (14159025) Suhur Saeed (17632212) Mustapha Aouida (417652) |
| author2_role | author author author author author author author author author author |
| author_facet | Alaa Hassanien (19420603) Nisar Ahmed (584617) Borbala Misfud (22997953) Hareb M. Al-Jabri (19420609) Sara Al-Marri (17075137) Tasneem Dalgamouni (19420606) Maryam Al-Merekhi (22997956) Kira Schipper (17316883) Imen Saadaoui (14159025) Suhur Saeed (17632212) Mustapha Aouida (417652) |
| author_role | author |
| dc.creator.none.fl_str_mv | Alaa Hassanien (19420603) Nisar Ahmed (584617) Borbala Misfud (22997953) Hareb M. Al-Jabri (19420609) Sara Al-Marri (17075137) Tasneem Dalgamouni (19420606) Maryam Al-Merekhi (22997956) Kira Schipper (17316883) Imen Saadaoui (14159025) Suhur Saeed (17632212) Mustapha Aouida (417652) |
| dc.date.none.fl_str_mv | 2025-09-22T09:00:00Z |
| dc.identifier.none.fl_str_mv | 10.1007/s10123-025-00715-x |
| dc.relation.none.fl_str_mv | https://figshare.com/articles/journal_contribution/Gene_expression_analysis_reveals_genes_related_to_heavy_metals_and_produced_water_exposure_in_Synechococcus_elongatus/31057117 |
| dc.rights.none.fl_str_mv | CC BY 4.0 info:eu-repo/semantics/openAccess |
| dc.subject.none.fl_str_mv | Biological sciences Genetics Microbiology Environmental sciences Environmental biotechnology Produced water Bioremediation Transcriptomics Wastewater Cyanobacteria |
| dc.title.none.fl_str_mv | Gene expression analysis reveals genes related to heavy metals and produced water exposure in Synechococcus elongatus |
| dc.type.none.fl_str_mv | Text Journal contribution info:eu-repo/semantics/publishedVersion text contribution to journal |
| description | <p dir="ltr">Produced water (PW), a major by-product of the petrochemical industry, contains a complex mixture of contaminants that limit its reuse and pose environmental risks if discharged untreated. Numerous treatment technologies have been developed to remediate this water, with bioremediation standing out as one of the most promising novel approaches. One such bioremediation method is through the application of cyanobacteria, which are able to remove pollutants such as heavy metals from produced water, although the mechanism by which the pollutants are removed is still unknown. In this study, a well-characterized cyanobacterium, S<i>ynechococcus elongatus</i>, was used as a model organism to establish a proof of concept for identifying genes responsive to PW exposure and heavy metal stress. RNA sequencing was performed to analyze transcriptomic changes in S. elongatus grown in BG-11 (control) and exposed to 3 mg/mL of iron (heavy metal (HM)) or 25% v/v PW in BG-11. Differential expression analysis revealed that 11 and 67 genes were ≥ fivefold upregulated, and 337 and 27 genes were ≥ fivefold downregulated under HM and PW exposure, respectively, compared to the control. Among the over-expressed genes, the plasma membrane transporter, nitrate ABC transporter permease, was identified, suggesting its important role in the bioremediation process of heavy metals from wastewater. These findings provide foundational insights into stress-responsive gene networks in cyanobacteria and inform future bioengineering strategies for enhancing bioremediation capabilities in <i>S. elongatus</i> and related strains.</p><h2 dir="ltr">Other Information</h2><p dir="ltr">Published in: International Microbiology<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1007/s10123-025-00715-x" target="_blank">https://dx.doi.org/10.1007/s10123-025-00715-x</a></p> |
| eu_rights_str_mv | openAccess |
| id | Manara2_8c479b5b86a9df3b36b2483c8e4f6b9d |
| identifier_str_mv | 10.1007/s10123-025-00715-x |
| network_acronym_str | Manara2 |
| network_name_str | Manara2 |
| oai_identifier_str | oai:figshare.com:article/31057117 |
| publishDate | 2025 |
| repository.mail.fl_str_mv | |
| repository.name.fl_str_mv | |
| repository_id_str | |
| rights_invalid_str_mv | CC BY 4.0 |
| spelling | Gene expression analysis reveals genes related to heavy metals and produced water exposure in Synechococcus elongatusAlaa Hassanien (19420603)Nisar Ahmed (584617)Borbala Misfud (22997953)Hareb M. Al-Jabri (19420609)Sara Al-Marri (17075137)Tasneem Dalgamouni (19420606)Maryam Al-Merekhi (22997956)Kira Schipper (17316883)Imen Saadaoui (14159025)Suhur Saeed (17632212)Mustapha Aouida (417652)Biological sciencesGeneticsMicrobiologyEnvironmental sciencesEnvironmental biotechnologyProduced waterBioremediationTranscriptomicsWastewaterCyanobacteria<p dir="ltr">Produced water (PW), a major by-product of the petrochemical industry, contains a complex mixture of contaminants that limit its reuse and pose environmental risks if discharged untreated. Numerous treatment technologies have been developed to remediate this water, with bioremediation standing out as one of the most promising novel approaches. One such bioremediation method is through the application of cyanobacteria, which are able to remove pollutants such as heavy metals from produced water, although the mechanism by which the pollutants are removed is still unknown. In this study, a well-characterized cyanobacterium, S<i>ynechococcus elongatus</i>, was used as a model organism to establish a proof of concept for identifying genes responsive to PW exposure and heavy metal stress. RNA sequencing was performed to analyze transcriptomic changes in S. elongatus grown in BG-11 (control) and exposed to 3 mg/mL of iron (heavy metal (HM)) or 25% v/v PW in BG-11. Differential expression analysis revealed that 11 and 67 genes were ≥ fivefold upregulated, and 337 and 27 genes were ≥ fivefold downregulated under HM and PW exposure, respectively, compared to the control. Among the over-expressed genes, the plasma membrane transporter, nitrate ABC transporter permease, was identified, suggesting its important role in the bioremediation process of heavy metals from wastewater. These findings provide foundational insights into stress-responsive gene networks in cyanobacteria and inform future bioengineering strategies for enhancing bioremediation capabilities in <i>S. elongatus</i> and related strains.</p><h2 dir="ltr">Other Information</h2><p dir="ltr">Published in: International Microbiology<br>License: <a href="https://creativecommons.org/licenses/by/4.0" target="_blank">https://creativecommons.org/licenses/by/4.0</a><br>See article on publisher's website: <a href="https://dx.doi.org/10.1007/s10123-025-00715-x" target="_blank">https://dx.doi.org/10.1007/s10123-025-00715-x</a></p>2025-09-22T09:00:00ZTextJournal contributioninfo:eu-repo/semantics/publishedVersiontextcontribution to journal10.1007/s10123-025-00715-xhttps://figshare.com/articles/journal_contribution/Gene_expression_analysis_reveals_genes_related_to_heavy_metals_and_produced_water_exposure_in_Synechococcus_elongatus/31057117CC BY 4.0info:eu-repo/semantics/openAccessoai:figshare.com:article/310571172025-09-22T09:00:00Z |
| spellingShingle | Gene expression analysis reveals genes related to heavy metals and produced water exposure in Synechococcus elongatus Alaa Hassanien (19420603) Biological sciences Genetics Microbiology Environmental sciences Environmental biotechnology Produced water Bioremediation Transcriptomics Wastewater Cyanobacteria |
| status_str | publishedVersion |
| title | Gene expression analysis reveals genes related to heavy metals and produced water exposure in Synechococcus elongatus |
| title_full | Gene expression analysis reveals genes related to heavy metals and produced water exposure in Synechococcus elongatus |
| title_fullStr | Gene expression analysis reveals genes related to heavy metals and produced water exposure in Synechococcus elongatus |
| title_full_unstemmed | Gene expression analysis reveals genes related to heavy metals and produced water exposure in Synechococcus elongatus |
| title_short | Gene expression analysis reveals genes related to heavy metals and produced water exposure in Synechococcus elongatus |
| title_sort | Gene expression analysis reveals genes related to heavy metals and produced water exposure in Synechococcus elongatus |
| topic | Biological sciences Genetics Microbiology Environmental sciences Environmental biotechnology Produced water Bioremediation Transcriptomics Wastewater Cyanobacteria |